| Project/Area Number |
20500320
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
EBIHARA Tatsuhiko National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門, 研究員 (00344119)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | シナプス / シナプス後肥厚部(PSD) / 大気圧走査電子顕微鏡(ASEM) / トランスジェニックマウス / PSD(シナプス後肥厚部) / IUEP(子宮内エレクトロポレーショング / IUEP(子宮内エレクトロポレーション) / 分子神経生物学 |
|---|
| Research Abstract |
For the purpose of stable visualizing the neuron and neural synapse, I made some transgenic mouse introduced by (fusion-) fluorescent protein's gene. And the completion of live brain neuron analysis using cre-loxP fluorescent mice and 2-photon microscope is in sight.
Using synapse specific fluorescent mice (made before this grant), I examined a possibility of the atmospheric scanning electron microscope (ASEM). This microscope realized the observation of cultured neuron in dH2O solution by EM. I examined staining condition, and analyzed fine structure of dendrite, spine and growth cone, and continue analysis for dynamic fine structure modification.
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