Physiological functions of nitric-oxide induced calcium-release in central nervous systems

• Project/Area Number: 20500333
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Nagasaki University
• Principal Investigator: KAKIZAWA Sho Nagasaki University, 薬学研究科, 准教授 (40291059)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 一酸化窒素 / カルシウム / 小脳 / プルキンエ細胞 / シナプス可塑性 / S-ニトロシル化 / プルキン工細胞 / 酸化シグナル / 老化 / 酸化ストレス / イオンチャネル

Research Abstract

The aim of this project is to examine the mechanism and functional role of nitric oxide-induce Ca2+ elevation in central neurons. In mouse cerebellar Purkinje cells, application of nitric oxide (NO) induced intracellular Ca^<2+> levels, and the elevation was revealed to be dependent on Ca^<2+> release from intracellular store. NO-induced Ca^<2+> increase was induced also by physiological patterns of neuronal activity which induces synaptic plasticity. Furthermore, the condition which blocks NO-induced Ca^<2+> increase also impaired the synaptic plasticity. There results indicate that NO-induced Ca^<2+> release is essential for the induction of synaptic plasticity in the cerebellum.

Research Products

• [Journal Article] TRIC-A Channels in Vascular Smooth Muscle Contribute to Blood Pressure Maintenance. (2011)
  • Author(s): Yamazaki D., Tabara Y., Kita S., Hanada H., Komazaki S., Naitou D., Mishima A., Nishi M., Yamamura H., Yamamoto S., Kakizawa S., Miyachi H., Yamamoto S., Miyata T., Kawano Y., Kamide K., Ogihara T., Hata A., Umemura S., Soma M., Takahashi N., Imaizumi Y., Miki, T., Iwamoto T., Takeshima H.
  • Journal Title: Cell Metabolism in press
  • Peer Reviewed
• [Journal Article] Mitsugumin 23 forms amassive bowl-shaped assembly and cation-conducting channel. (2011)
  • Author(s): Venturi E., Mio K., Nishi M., Ogura T., Moriya T., Pitt S., Okuda K., Kakizawa S., Sitsapesan R., Sato C., Takeshima H.
  • Journal Title: Biochemistry 50 Pages: 2623-2632
  • Peer Reviewed
• [Journal Article] Protein oxidation inhibits NO-mediated signaling pathway for synaptic plasticity (2011)
  • Author(s): Kakizawa S, Shibazaki M, Mori N.
  • Journal Title: Neurobiology of Aging Volume: (印刷中)
  • NAID: 120006984470
  • Peer Reviewed
• [Journal Article] Mitsugumin 23 forms a massive bowl-shaped assembly and cation-conducting channel (2011)
  • Author(s): Venturi E, Mio K, Nishi M, Ogura T, Moriya T, Pitt S, Okuda K, Kakizawa S, Sitsapesan R, Sato C, Takeshima H
  • Journal Title: Biochemistry Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Shibazaki, M. & Mori, N.Protein oxidation inhibits NO-mediated signaling pathway for synaptic plasticity. (2010)
  • Author(s): 柿澤昌
  • Journal Title: Neurobiology of Aging (in press; accepted on2010/04/20)
  • Peer Reviewed
• [Journal Article] I型インシュリン様成長因子受容体 (2010)
  • Author(s): 柿澤昌
  • Journal Title: 生体の科学 61巻5号 Pages: 386-387
  • Peer Reviewed
• [Journal Article] I型インシュリン様成長因子受容体 (2010)
  • Author(s): 柿澤昌
  • Journal Title: 生体の科学 Volume: 61 Pages: 386-387
• [Journal Article] Functional crosstalk between cell-surface and intracellular channels mediated by junctophilins essential for neuronal functions. (2008)
  • Author(s): Kakizawa S., Moriguchi, S., Ikeda, A., Iino, M., Takeshima, H.
  • Journal Title: Cerebellum 7 Pages: 385-391
  • Peer Reviewed
• [Journal Article] Functional crosstalk between cell-surface and intracellular channels mediated by junctophilins essential for neuronal functions (2008)
  • Author(s): Kakizawa S, Moriguchi S, Ike da A, Iino M & Takeshima H
  • Journal Title: Cerebellum 7 Pages: 385-391
  • Peer Reviewed
• [Presentation] Protein oxidation inhibits nitric oxide-mediated signaling pathway essential for synaptic plasticity. (2011)
  • Author(s): Kakizawa S., Shibazaki, M., Mori, N., Takeshima, H.
  • Organizer: Biophysical Society 55th Annual Meeting.
  • Place of Presentation: Baltimore MD, U.S.A.
  • Year and Date: 2011-04-06
• [Presentation] Protein Oxidation inhibits Nitric Oxide-Dependent Signaling Pathway Essential for Synaptic Plasticity in the CNS. (2011)
  • Author(s): 柿澤昌
  • Organizer: The 6th International Symposium on Receptor Mechanisms, Signal Transduction and Drug Effects. Signal Transduction and Drug Effects.
  • Place of Presentation: Kyoto
  • Year and Date: 2011-04-02
• [Presentation] 一酸化窒素シグナル依存的シナプス可塑性と活性酸素種による制御 (2011)
  • Author(s): 柿澤昌
  • Organizer: 第84回日本薬理学会年会シンポジウム(オーガナイザー:西田基宏、松本明)
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-24
• [Presentation] 一酸化窒素シグナル依存的シナプス可塑性と活性酸素種による制御 (2011)
  • Author(s): 柿澤昌
  • Organizer: 第84回日本薬理学会年会シンポジウム
  • Place of Presentation: 震災のため誌上開催(招待講演)
  • Year and Date: 2011-03-24
• [Presentation] リアノジン受容体を介した一酸化窒素によるカルシウム放出機構 (2011)
  • Author(s): 山澤徳志子、柿澤昌、小山田英人、村山尚、櫻井隆、小口勝司、飯野正光
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-23
• [Presentation] PROTEIN OXIDATION INHIBITS NITRIC OXIDE-MEDIATED SIGNALING PATHWAY ESSENTIAL FOR SYNAPTIC PLASTICITY (2011)
  • Author(s): Kakizawa S, Shibazaki M, Mori N, Takeshima H
  • Organizer: Biophysical Society 55th Annual Meeting
  • Place of Presentation: Baltimore Convention Center (Baltimore, MD, U.S.A.)
  • Year and Date: 2011-03-06
• [Presentation] Protein Oxidation inhibits Nitric Oxide-Induced Signaling Pathway Essential for Synaptic Plasticity in the CNS. (2011)
  • Author(s): 柿澤昌
  • Organizer: The 1st AACL Meeting.
  • Place of Presentation: Fukuoka.
  • Year and Date: 2011-02-18
• [Presentation] Protein Oxidation inhibits Nitric Oxide-Induced Signaling Pathway Essential for Synaptic Plasticity in the CNS (2011)
  • Author(s): Kakizawa S
  • Organizer: The 1st AACL Meeting
  • Place of Presentation: ホテル福岡ガーデンパレス(福岡市)(招待講演)
  • Year and Date: 2011-02-18
• [Presentation] 受容体アダプター分子欠損マウスの小脳プルキンエ細胞における細胞内Ca放出系の機能阻害 (2010)
  • Author(s): 柿澤昌
  • Organizer: 第4回トランスポーター研究会九州部会
  • Place of Presentation: 佐世保
  • Year and Date: 2010-09-11
• [Presentation] 受容体アダプター分子欠損マウスの小脳プルキンエ細胞における細胞内Ca放出系の機能阻害 (2010)
  • Author(s): 柿澤昌
  • Organizer: 第4回トランスポーター研究会九州部会
  • Place of Presentation: ハウステンボス(佐世保市)
  • Year and Date: 2010-09-11
• [Presentation] 森 望.老齢マウス小脳プルキンエ細胞における酸化ストレスによる神経可塑性の低下 (2010)
  • Author(s): 柿澤昌、柴崎晶彦、大神和子
  • Organizer: 日本基礎老化学会第33回大会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-06-18
• [Presentation] 老齢マウス小脳プルキンエ細胞における酸化ストレスによる神経可塑性の低下 (2010)
  • Author(s): 柿澤昌、柴崎晶彦、大神和子、森望
  • Organizer: 日本基礎老化学会第33回大会
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2010-06-18
• [Presentation] 中枢ニューロンにおける受容体アダプター分子によるイオン輸送体機能制御と生理学的意義 (2009)
  • Author(s): 柿澤昌
  • Organizer: トランスポーター研究会第3回九州部会シンポジウム(オーガナイザー:古川龍彦)
  • Place of Presentation: 鹿児島
  • Year and Date: 2009-11-21
• [Presentation] 中枢ニューロンにおける受容体アダプター分子によるイオン輸送体機能制御と生理学的意義 (2009)
  • Author(s): 柿澤昌
  • Organizer: トランスポーター研究会第3回九州部会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2009-11-21
• [Presentation] リアノジン受容体を介した一酸化窒素によるカルシウム放出機構 (2009)
  • Author(s): 山澤徳志子、柿澤昌、小山田英人、村山尚、櫻井隆、小口勝司、飯野正光
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] 脳は使わないと衰える?-小脳興奮性シナプスの活動依存的維持機構 (2008)
  • Author(s): 柿澤昌
  • Organizer: 第4回長崎ニューロサイエンス研究会
  • Place of Presentation: 長崎
  • Year and Date: 2008-10-04
• [Presentation] 脳は使わないと衰える?-小脳興奮性シナプスの活動依存的維持機構 (2008)
  • Author(s): 柿澤 昌
  • Organizer: 第4回長崎ニューロサイエンス研究会
  • Place of Presentation: 長崎市
  • Year and Date: 2008-10-04
• [Presentation] Junctophilin-mediated functional crosstalk between ryanodine receptors and SK channels essential for long-term depression in the cerebellum. (2008)
  • Author(s): Kakizawa S., Kishimoto Y., Hashimoto K., Miyazaki T., Furutani K., Shimizu H., Fukaya M., Nishi M., Sakagami H., Ikeda A., Kondo K., Kano M., Iino M., Takeshima H.
  • Organizer: The 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry.
  • Place of Presentation: Shanghai, China.
  • Year and Date: 2008-06-25
• [Presentation] リアノジン受容体を介した一酸化窒素によるカルシウム放出機構 (2008)
  • Author(s): 山澤徳志子、柿澤昌、小山田英人、村山尚、櫻井隆、小口勝司、飯野正光
  • Organizer: 平成20年筋生理の集い
  • Place of Presentation: 東京
• [Presentation] 一酸化窒素(NO)による細胞内Ca2+動員機構 (2008)
  • Author(s): 山澤徳志子、柿澤昌、小山田英人、村山尚、櫻井隆、小口勝司、飯野正光
  • Organizer: 第119回日本薬理学会関東部会
  • Place of Presentation: 東京
• [Presentation] Regulation of climbing fiber territory by the carboxyl terminal of GluR?2 at parallel fiber synapses incerebellar Purkinje cells (2008)
  • Author(s): 植村健、柿澤昌、山崎美和子、崎村建司、渡辺雅彦、飯野正光、三品昌美
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] Junctophilin-mediated functional crosstalk between ryanodine receptors and SK channels essential for long-term depression in the cerebellum (2008)
  • Author(s): Kakizawa, S., 他14名
  • Organizer: The 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry
  • Place of Presentation: 中国、上海
• [Book] 8章恒常性(分担執筆)新医歯薬学系学生のための基礎生命科学(竹島浩編) (2011)
  • Author(s): 柿澤昌
  • Publisher: 京都廣川書店(印刷中)
• [Book] Ca2+シグナルとイオンチャネル総論(トランスポートソームの世界).(金井好克、竹島浩、森泰生、久保義弘編、) (2011)
  • Author(s): 竹島浩、柿澤昌
  • Publisher: 京都廣川書店(印刷中)
• [Book] 小胞体Ca2+放出チャネルファミリー(トランスポートソームの世界)(金井好克、竹島浩、森泰生、久保義弘編、) (2011)
  • Author(s): 竹島浩、柿澤昌
  • Publisher: 京都廣川書店(印刷中)
• [Book] 結合膜構造とチャネルミクロアセンブリ(トランスポートソームの世界)(金井好克、竹島浩、森泰生、久保義弘編、) (2011)
  • Author(s): 竹島浩、柿澤昌
  • Publisher: 京都廣川書店(印刷中)
• [Book] 新医歯薬系学生のための基礎生命科学(第二版) (2011)
  • Author(s): 竹島浩、柿澤昌
  • Publisher: 京都廣川書店(印刷中)
• [Remarks] ホームページ等
• [Remarks] 京都大学学術情報リポジトリ
  • URL: http://repository.kulib.kyoto-u.ac.jp
• [Remarks] 長崎大学学術研究成果リポジトリ
  • URL: http://naosite.lb.nagasaki-u.ac.jp/ds