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Studies on the spatiotemporal regulation of vesicle recruitment for exocytosis

Research Project

Project/Area Number 20500342
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionSophia University

Principal Investigator

KUMAKURA Konosuke  Sophia University, 理工学部, 教授 (70129790)

Co-Investigator(Kenkyū-buntansha) SASAKAWA Nobuyuki  上智大学, 理工学部, 教授 (20187107)
Research Collaborator RIVA Alessandro  University of Cagliari School of medicine, Professor
HAYASHI Mitunori  上智大学, 理工学部, 特別研究員
田村 和季  上智大学, 大学院・理工学研究科, 院生
姜 裕貴  上智大学, 大学・院理工学研究科, 院生
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords開口分泌 / 顆粒供給 / 細胞骨格系蛋白質 / 空間的顆粒分布 / クロマフィン細胞 / 細胞骨格蛋白質 / 細胞骨格系タンパク質 / ミオシンATPアーゼ
Research Abstract

Present studies in chromaffin cells revealed that vesicle recruitment for exocytosis from the reserve pool to the releasable pool is a process with the intracellular translocation of the vesicles which affect the spatial localization of vesicles. Roles of Myosin V as a motor protein, and of actin filaments as rails for this recruitment were also shown.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (20 results)

All 2011 2010 2009 2008

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (16 results)

  • [Journal Article] Dissociation of inositol polyphosphates from the C2B domain of synaptotagmin facilitates spontaneous release of catecholamines in adrenal chromaffin cells. A suggestive evidence of a fusion clamp by synaptotagmin.2011

    • Author(s)
      Nobuyuki Sasakawa, Mica Ohara-Imaizumi, Mitsunori Fuuda, Hiroyuki Kabayama, Katsuhiko Mikoshiba, Konosuke Kumakura
    • Journal Title

      Neuropharmacology 60

      Pages: 1364-1370

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dissociation of inositol polyphosphates from the C2B domain of synaptotagmin facilitates spontaneous release of catecholamines in adrenal chromaffin cells. A suggestive evidence of a fusion clamp by synaptotagmin2011

    • Author(s)
      Nobuyuki Sasakawa
    • Journal Title

      Neuropharmacology

      Volume: 60 Pages: 1364-1370

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Sodium Channel-Targeted Conotoxins on Catecholamine Release in Adrenal Chromaffin Cells.2008

    • Author(s)
      Elsie C.Jimenez, Nobuyuki Sasakawa, Konosuke Kumakura
    • Journal Title

      Phillipine Journal of Science 137

      Pages: 127-132

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Sodium Channel-Targeted Conotoxins on Catecholamine Release in Adrenal Chromaffin Cells.2008

    • Author(s)
      Elsie C. Jimenez
    • Journal Title

      Phillipine Journal of Science 137

      Pages: 127-132

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Vesicle movement for exocytosis in adrenal chromaffin cells : Function and Structure2010

    • Author(s)
      Konosuke Kumakura
    • Organizer
      10^<th> Italy-Japan Symposium in Miyazaki on New Frontiers of the Research on Human Kealth, Food Sciences and Energy Production
    • Place of Presentation
      宮崎
    • Year and Date
      2010-11-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウスクロマフィン細胞からの開口分泌におけるシンタキシンIA-CaMKII相互作用の役割2010

    • Author(s)
      姜裕貴
    • Organizer
      第58回日本薬理学会年会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] ウシ副腎髄質クロマフィン細胞における分泌顆粒の細胞内局在、及び動態の4次元解析2010

    • Author(s)
      林光紀
    • Organizer
      第58回日本薬理学会年会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] ウシ副腎髄質クロマフィン細胞における分泌顆粒の細胞内局在、及び動態の4次元解析2010

    • Author(s)
      林光紀
    • Organizer
      第58回 日本薬理学会年会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウスクロマフィン細胞からの開口分泌におけるシンタキシンIA-CaMKII相互作用の役割2010

    • Author(s)
      姜裕貴
    • Organizer
      第58回 日本薬理学会年会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] Vesiscle Mobility and Transmitter Release2009

    • Author(s)
      Konosuke Kumakura
    • Organizer
      From Neurotransmitters to Neuroepigenomics
    • Place of Presentation
      Chicago, USA (Invited lecture)
    • Year and Date
      2009-10-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Vesiscle Mobility and Transmitter Release(Invited lecture)2009

    • Author(s)
      Konosuke Kumakura
    • Organizer
      From Neurotransmitters to Neuroepigenomics
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2009-10-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] 4 dimensional characteristics of the movement and location of the secretory granules in bovine adrenal chromaffin cells.2009

    • Author(s)
      林光紀
    • Organizer
      第52回日本神経化学会大会
    • Place of Presentation
      渋川市伊香保
    • Related Report
      2010 Final Research Report
  • [Presentation] 牛副腎クロマフィン細胞のドーパミン受容体を介した生理的制御作用2009

    • Author(s)
      田村和季
    • Organizer
      第82回日本薬理学会年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Involvement of myosin in the vesicle recruitment for exocytosis : Studies with amperometry and HRSEM.Gordon Research Conferences.2009

    • Author(s)
      Konosuke Kumakura
    • Organizer
      Salivary Glands & Exocrine Secretion.
    • Place of Presentation
      Galveston, TX, USA (Invited Lecture)
    • Related Report
      2010 Final Research Report
  • [Presentation] 4 dimensional characteristics of the movement and location of the secretory granules in bovine adrenal chromaffin cells2009

    • Author(s)
      林光紀
    • Organizer
      第52回 日本神経化学会大会
    • Place of Presentation
      群馬県渋川市伊香保町
    • Related Report
      2009 Annual Research Report
  • [Presentation] Involvement of myosin in the vesicle recruitment for exocytosis : Studies with amperometry and HRSEM2009

    • Author(s)
      Konosuke Kumakura(Invited Lecture)
    • Organizer
      Gordon Research Conferences. Salivary Glands & Exocrine Secretion
    • Place of Presentation
      Galveston, TX, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] ウシ副腎髄質クロマフィン細胞における分泌顆粒動態の4次元解析2008

    • Author(s)
      林光紀
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京
    • Year and Date
      2008-07-10
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Possible involvement of myosin-ATPase in the vesicle recruitment for exocytosis in adrenal chromaffin cells : Studies with scanning electron microscopy2008

    • Author(s)
      Konosuke Kumakura
    • Organizer
      第51回日本神経化学会大会
    • Place of Presentation
      富山
    • Related Report
      2010 Final Research Report
  • [Presentation] Characterization of exocytotic events and the dynamics of secretory vesicles in single mouse chromaffin cells.2008

    • Author(s)
      Nobuyuki Sasakawa
    • Organizer
      第51回日本神経化学会大会
    • Place of Presentation
      富山
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Possible involvement of myosin-ATPase in the vesicle recruitment for exocytosis in adrenal chromaffin cells : Studies with scanning electron microscopy.2008

    • Author(s)
      Konosuke Kumakura
    • Organizer
      第51回日本神経化学会大会
    • Place of Presentation
      富山
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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