Identification of membrane targets for 15-deoxy Δ^<12,14>-prostaglandin J_2

• Project/Area Number: 20500346
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Himeji Dokkyo University
• Principal Investigator: YAGAMI Tatsurou Himeji Dokkyo University, 薬学部, 教授 (00363812)
• Co-Investigator(Kenkyū-buntansha): TAKASE Kenkichi 姫路獨協大学, 薬学部, 講師 (80381474)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経科学 / 薬理学 / 脳 / 脳神経疾患 / 脂質 / 15d-Δ^<12,14>-PGJ_2 / Neuron / Plasma membrane / Reactive Oxygen species / apoptosis / glycolytic enzyme / molecular chaperone / Cytoskeleton

Research Abstract

We found that specific binding sites of 15-deoxy-Δ^<12,14>-prostaglandin J_2 (15d-PGJ_2) was involved in the neurodegeneration. A proteomic approach was used to identify protein targets for 15d-PGJ_2 in the plasma membrane. By using biotinylated 15d-PGJ_2, eleven proteins were identified as biotin-positive spots and classified into three different functional proteins : glycolytic enzymes (Enolase2, pyruvate kinase M1 (PKM1) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH)), molecular chaperones (heat shock protein 8 and T-complex protein 1 subunit α), cytoskeletal proteins (Actin β, F-actin-capping protein, Tubulin β and Internexin α). GAPDH, PKM1 and Tubulin β are Aβ-interacting proteins.

Research Products

• [Journal Article] 15-deoxy-Δ12,14- prostaglandin J2 enhanced the anti-tumor activity of camptotecin against renal cell carcinoma independently of topoisomerase-II and PPARγ pathways. (2011)
  • Author(s): Yamamoto Y, Fujita M, Kouhma H, Yamamori M, Nakamura T, Okamura N, Yagami T
  • Journal Title: Biochim Biophys Res Commun (in press)
• [Journal Article] Identification of novel protein targets for modification by 15-deoxy-Δ12,14- prostaglandin J2 in neuronal plasma membrane. (2011)
  • Author(s): Yamamoto Y, Takase K, Kishino J, Fujita M, Okamura N, Sakaeda T, Fujimoto M, Yagami T
  • Journal Title: PLoS ONE 6(3)
• [Journal Article] Identification of novel protein targets for modification by 15-deoxy-Δ^<12,14>-prostaglandin J_2 in neuronal plasma membrane. (2011)
  • Author(s): Yamamoto Y, Yagami T, et al., (8名,8番、責任著者)
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 2 induces apoptosis in the early primary culture of rat cortical neurons. (2010)
  • Author(s): Yagami T, Takase K, Yamamoto Y, Ueda K, Takasu N, Okamura N, Sakaeda T, Fujimoto M
  • Journal Title: Exp Cell Res. 316(14) Pages: 2278-2290
• [Journal Article] Fibroblast growth factor 2 induces apoptosis in the early primary culture of rat cortical neurons. (2010)
  • Author(s): Yagami T, et al., (8名,1番、責任著者)
  • Journal Title: Experimental Cell Research Volume: 316 Pages: 2278-2290
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 2 induces apoptosis in the early primary culture of rat cortical neurons. (2010)
  • Author(s): Yagami T, et al., (8名,1番)
  • Journal Title: Experimental Cell Research (In press)
  • Peer Reviewed
• [Journal Article] Sex-specific 24-h acetylcholine release profile in the medial prefrontal cortex : Simultaneous measurement of spontaneous locomotor activity in behaving rats. (2009)
  • Author(s): Takase K, Yagami T, et al., (4名,2番)
  • Journal Title: Neuroscience 159 Pages: 7-15
  • Peer Reviewed
• [Journal Article] DOPA cyclohexyl ester, a DOPA antagonist, blocks the depressor responses elicited by microinjections of nicotine into the nucleus tractus solitarii of rats (2008)
  • Author(s): Murota Y, Yagami T, et al., (7名, 5番)
  • Journal Title: Neurosci Lett. 442 Pages: 114-117
  • Peer Reviewed
• [Journal Article] Quantitative proteomic analysis to discover potential diagnostic markers and therapeutic targets in human renal cell carcinoma (2008)
  • Author(s): Okamura N, Yagami T, et al., (18名, 14番)
  • Journal Title: Proteomics 8 Pages: 3194-3203
  • NAID: 130006996715
  • Peer Reviewed
• [Journal Article] 線虫C.elegans における新規7回膜貫通型受容体は揮発性ベンズアルデヒドに対する忌避応答に関与する (2008)
  • Author(s): 青木令奈, 矢上達郎, et al., (18名, 14番)
  • Journal Title: 横浜医学 59 Pages: 117-123
  • Peer Reviewed
• [Presentation] 腎臓がん細胞における15-deoxy-Δ12,14-prostaglandin J2の抗腫瘍効果 (2011)
  • Author(s): 田路千明、藤田恵、澤田京子、山森元博、山本泰弘、高瀬堅吉、矢上達郎、岡村昇
  • Organizer: 日本薬学会第131年会 29P-0780
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-29
• [Presentation] 腎臓がん細胞におけるトログリタゾンの抗腫瘍効果に対するp38 MAPK経路の役割 (2011)
  • Author(s): 藤尾弥希、藤田恵、田路千明、澤田京子、山森元博、山本泰弘、高瀬堅吉、矢上達郎、岡村昇
  • Organizer: 日本薬学会第131年会 29P-0781
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-29
• [Presentation] 腎臓がん細胞におけるトログリタゾンの抗腫瘍効果に対するp38 MAPK経路の役割 (2011)
  • Author(s): Yagami T, et al., (9名,8番)
  • Organizer: 日本薬学会第131年会(29P-0781)
  • Place of Presentation: 静岡県コンベンションアーツセンター(静岡)
  • Year and Date: 2011-03-29
• [Presentation] 臓がん細胞における15-deoxy-Δ12,14-prostaglandin J2の抗腫瘍効果 (2011)
  • Author(s): Yagami T, et al., (8名,7番)
  • Organizer: 日本薬学会第131年会(29P-0780)
  • Place of Presentation: 静岡県コンベンションアーツセンター(静岡)
  • Year and Date: 2011-03-29
• [Presentation] Camptothecin enhanced anti-tumor activity of 15-deoxy-Δ12,14-prostaglandin J2 in renal cell carcinoma. (2011)
  • Author(s): Yamamoto Y, Takase T, Fujita M, Okamura N, Sakaeda T, Yagami T
  • Organizer: 第84回日本薬理学会年会 P3J19-2(J Pharmacol Sci. 2011 ; 115(Sup 1) : 278P)
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-24
• [Presentation] Camptothecin enhanced anti-tumor activity of 15-deoxy-Δ12,14-prostaglandin J2 in renal cell carcinoma. (2011)
  • Author(s): Yamamoto Y, Yagami T, et al., (6名,6番、責任著者)
  • Organizer: 第84回日本薬理学会年会(P3J19-2)
  • Place of Presentation: 横浜パシフィコ(神奈川)
  • Year and Date: 2011-03-24
• [Presentation] Biological Effects of Blood Glucose Level Control (2010)
  • Author(s): Yagami T(1名,1番)
  • Organizer: Pharmaceutical Sciences World Congress 2010
  • Place of Presentation: Ernest N.Memorial Convention Center (New Orleans)(招待講演)
  • Year and Date: 2010-11-16
• [Presentation] Maternal deprivation from the later infant stage to the early juvenile stage causes multiple abnormal behaviors related to autistic disorder only in mal e rats. (2010)
  • Author(s): Takase K, Yagami T, et al., (3名,3番、責任著者)
  • Organizer: 第33回日本神経科学大会(O2-7-3-2)
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 2010-09-03
• [Presentation] Identification of novel protein targets for 15-deoxy-Δ12,14-prostaglandin J2 in neuronal plasma membranes. (2010)
  • Author(s): Yagami T, Yamamoto Y, Takase K, Okamura N, Sakaeda T
  • Organizer: 第33回日本神経科学大会 O1-7-3-2
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] Identification of novel protein targets for 15-deoxy-Δ^<12,14>-prostaglandin J_2 in neuronal plasma membranes. (2010)
  • Author(s): Yagami T, et al., (5名,1番、責任著者)
  • Organizer: 第33回日本神経科学大会(O1-7-3-2)
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 2010-09-02
• [Presentation] 腎臓がん細胞におけるトログリタゾンの抗腫瘍活性の検討 (2010)
  • Author(s): 藤田恵、田路千明、澤田京子、山本泰弘、高瀬堅吉、矢上達郎、岡村昇
  • Organizer: 日本薬学会第130年会 28P-pm362Q
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] 腎臟がん細胞におけるトログリタゾンの抗腫瘍活性の検討 (2010)
  • Author(s): Yagami T, et al., (7名,6番)
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山コンベンションセンター(岡山)
  • Year and Date: 2010-03-28
• [Presentation] Hypoxia induced upregulation of VEGF in renal cell carcinoma via VHL-independent pathways. (2010)
  • Author(s): Yamamoto Y, Takase K, Matsumoto J, Yoshida T, Nakamura T, Okamura N, Sakaeda T, Okumura K, Yagami T
  • Organizer: 第83回日本薬理学会年会 P3J-21-3(J Pharmacol Sci. 2010 ; 112(Suppl) : 260P)
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-18
• [Presentation] 腎臓癌細胞における低酸素誘導VEGF発現増強はVHL非依存的経路を介する (2010)
  • Author(s): Yagami T, et al., (9名,9番)
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-03-18
• [Presentation] Identification of novel protein targets for modification by 15-deoxy-△12,14-prostaglandin J2 in neuronal plasma membranes. (2009)
  • Author(s): Yagami T, Takase K, Yamamoto Y
  • Organizer: 第32回日本神経科学大会 P3-l12(Neurosci Res. 2009 ; 65 (Suppl) : S245)
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] 神経細胞膜における15-deoxy-△12,14-prostaglandin J2標的タンパク質の同定 (2009)
  • Author(s): Yagami T, et al., (3名,1番)
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2009-09-18
• [Presentation] Early maternal deprivation enhances anxiety and stereotypy, but reduces sociality in adult male rats. (2009)
  • Author(s): Yagami T, et al., (3名,2番)
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2009-09-18
• [Presentation] A novel seven-transmembrane domain receptor DCAR-1 mediates avoidance response to a water-soluble repellent in C.elegans (2009)
  • Author(s): Aoki R, Yagami T, et al., (10名, 2番)
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-03-17
• [Presentation] A possible involvement of 15-deoxy-Δ12,14-prostaglandin J2 in amyloidosis (2008)
  • Author(s): Yagami T, Takase K
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 2008-07-11
• [Presentation] Some DOPS derivatives mimic DOPAergic cardiovascular response in rats (2008)
  • Author(s): Fujii M, Yagami T, et al., (7名, 3番)
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 2008-07-10
• [Presentation] A possible involvement of 15-deoxy-Δ12,14-prostaglandin J2 in amyloidosis. (2008)
  • Author(s): Yagami T, Takase K
  • Organizer: 第31回日本神経科学大会 P3-q13(Neurosci Res. 2008 ; 61(Suppl) : S146)
  • Place of Presentation: 東京
• [Presentation]
  • Author(s): Yagami T
  • Organizer: Biological Effects of Blood Glucose Level Control Pharmaceutical Sciences World Congress 20101114-20101118
  • Place of Presentation: New Orleans, Louisiana, USA(invited)
• [Remarks] ホームページ
  • URL: http://www.himeji-du.ac.jp/faculty/dp_pharm/pharm/ph3/
• [Remarks]
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• [Remarks]
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• [Remarks]
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• [Patent(Industrial Property Rights)] 細胞変性調節活性の測定方法 (2009)
  • Inventor(s): 矢上達郎, 他2名
  • Industrial Property Rights Holder: 矢上達郎
  • Industrial Property Number: 2009-239184
  • Filing Date: 2009-10-16