| Project/Area Number |
20500346
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Himeji Dokkyo University |
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Principal Investigator |
YAGAMI Tatsurou Himeji Dokkyo University, 薬学部, 教授 (00363812)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASE Kenkichi 姫路獨協大学, 薬学部, 講師 (80381474)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 神経科学 / 薬理学 / 脳 / 脳神経疾患 / 脂質 / 15d-Δ^<12,14>-PGJ_2 / Neuron / Plasma membrane / Reactive Oxygen species / apoptosis / glycolytic enzyme / molecular chaperone / Cytoskeleton |
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| Research Abstract |
We found that specific binding sites of 15-deoxy-Δ^<12,14>-prostaglandin J_2 (15d-PGJ_2) was involved in the neurodegeneration. A proteomic approach was used to identify protein targets for 15d-PGJ_2 in the plasma membrane. By using biotinylated 15d-PGJ_2, eleven proteins were identified as biotin-positive spots and classified into three different functional proteins : glycolytic enzymes (Enolase2, pyruvate kinase M1 (PKM1) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH)), molecular chaperones (heat shock protein 8 and T-complex protein 1 subunit α), cytoskeletal proteins (Actin β, F-actin-capping protein, Tubulin β and Internexin α). GAPDH, PKM1 and Tubulin β are Aβ-interacting proteins.
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