Regulation of the maturation of cerebellar synapses by PKN

• Project/Area Number: 20500354
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Gunma University
• Principal Investigator: YASUDA Hiroki Gunma University, 大学院・医学系研究科, 准教授 (60294071)
• Co-Investigator(Renkei-kenkyūsha): MUKAI Hideyuki 神戸大学, 大学院・医学研究科, 准教授 (80252758)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: KN1 / シナプス可塑性 / 長期抑圧 / 海馬 / 小脳 / 電気生理 / PKN1 / ヘテロシナプス性長期抑圧 / サイレントシナプス / ノックアウトマウス / PKN / シナプス長期抑圧

Research Abstract

I investigated the role of PKN1 in synaptic transmission and synaptic plasticity in the central nervous system electrophysiologically using PKN1a knockout (KO) mice. I found LTD was enhanced and heterosynaptic LTD was induced in the hippocampus of developing PKN1a KO mice and synaptic transmission was more immature. I concluded that PKN1 is promoting synaptic maturation by inhibiting LTD during development.

Research Products

• [Journal Article] Hypotonic Swelling-Induced Activation of Pkn1 Mediates Cell Survival in Cardiac Myocytes (2010)
  • Author(s): 向井秀幸、小野功貴, 他
  • Journal Title: Am J Physiol Heart Circ Physiol. Vol.300、No.1 Pages: 191-200
  • Peer Reviewed
• [Journal Article] Accumulation of tumor-suppressor PTEN in Alzheimer neurofibrillary tangles (2010)
  • Author(s): 向井秀幸、小野功貴, 他
  • Journal Title: Neurosci Lett. Vol.471、No.1 Pages: 20-24
  • NAID: 120003290064
  • Peer Reviewed
• [Journal Article] Development of an intracellularly acting inhibitory peptide selective for PKN. (2009)
  • Author(s): 志賀一博、小野功貴、向井秀幸, 他
  • Journal Title: Biochem J. Vol.425、No.2 Pages: 445-543
  • Peer Reviewed
• [Journal Article] A peptidyl-prolyl isomerase, FKBP12, accumulates in Alzheimer neurofibrillary tangles (2009)
  • Author(s): 小野功貴、向井秀幸, 他
  • Journal Title: Neurosci.Lett. Vol.459、No.2 Pages: 96-99
  • Peer Reviewed
• [Journal Article] Roles of endocannabinoids in heterosynaptic long-term depression of excitatory synaptic transmission in visual cortex of young mice (2008)
  • Author(s): 黄艶,安田浩樹,アブドルラハン・サリヒ、津本忠治、
  • Journal Title: Journal of Neuroscience Vol.28、No.28 Pages: 7074-7083
  • Peer Reviewed
• [Journal Article] Regulation of excitability and plasticity by endocannabinoids and PKA in developing hippocampus (2008)
  • Author(s): 安田浩樹、黄艶、津本忠治
  • Journal Title: Proceeding of the National Academy of Science of the United States of America Vol.105、No.8 Pages: 3106-3111
  • Peer Reviewed
• [Journal Article] Calcineurin-mediated dephosphorylation of c-Jun Ser-243 is required for c-Jun protein stability and cell transformation (2008)
  • Author(s): 吉川潮、向井秀幸, 他
  • Journal Title: Oncogene Vol.27、No.17 Pages: 2422-2429
  • Peer Reviewed
• [Journal Article] Roles of endocannabinoids in heterosynaptic long-term depression of excitatory synaptic transmission in visual cortex of young mice. (2008)
  • Author(s): 黄艶
  • Journal Title: Journal of Neuroscience 28 Pages: 7074-7083
  • Peer Reviewed
• [Presentation] ドレブリンのアイソフォーム変換は海馬依存的学習とシナプス可塑性に必要である (2011)
  • Author(s): 児島伸彦、安田浩樹, 他
  • Organizer: 第84回日本薬理学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-03-23
• [Presentation] Neuron-specific drebrin isoform is necessary for hippocampus dependent learning in adulthood but not in young age of mice (2010)
  • Author(s): 児島伸彦、安田浩樹, 他
  • Organizer: Neuroscience 2010 Society for Neuroscience 40th Annual Meeting
  • Place of Presentation: サンディエゴコンベンションセンター(サンディエゴ、米国)
  • Year and Date: 2010-11-16
• [Presentation] Neuron-specific drebrin isoform is necessary for hippocampus-dependent learning in adulthood but not in young age of mice (2010)
  • Author(s): N.KOJIMA, H.YASUDA, et al
  • Organizer: Neuroscience 2010 Society for Neuroscience 40^<th> Annual Meeting
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] ドレブリンアイソフォーム変換不全マウスにおける海馬シナプス可塑性と海馬依存的学習の障害 (2010)
  • Author(s): 児島伸彦、安田浩樹, 他
  • Organizer: Neuro2010 第33回日本神経科学学会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-09-04
• [Presentation] ドレブリンアイソフォーム変換不全マウスにおける海馬シナプス可塑性と海馬依存的学習の障害 (2010)
  • Author(s): 児島伸彦、安田浩樹, 等
  • Organizer: Neuro2010第33回日本神経科学学会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-09-04
• [Presentation] Lrfn2/SALM1, a synaptic leucine-rich repeat transmembrane molecule is required for proper synapse maturation and brain function (2009)
  • Author(s): 守村直子、安田浩樹, 他
  • Organizer: 第32回日本神経科学学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-17
• [Presentation] Synaptic maturation by PKN during development (2009)
  • Author(s): 安田浩樹, 他
  • Organizer: 第32回日本神経科学学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-17
• [Presentation] Lrfn2/SALM1, a synaptic leucine-rich repeat transmembrane molecule is required for proper synapse maturation and brain function (2009)
  • Author(s): 守村直子、太田摩耶、安田浩樹, 他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Synaptic maturation by PKN during development (2009)
  • Author(s): 安田浩樹
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] SPECIFIC ROLE OF NEURONAL ISOFORM OF DREBRIN IN HIPPOCAMPAL SYNAPTIC PLASTICITY AND FEAR MEMORY (2009)
  • Author(s): 児島伸彦、安田浩樹, 他
  • Organizer: The XXXVI International Congress of Physiological Sciences (IUPS2009)
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2009-07-28
• [Presentation] SPECIFIC ROLE OF NEURONAL ISOFORM OF DREBRIN IN HIP POCAMPAL SYNAPTIC PLASTICITY AND FEAR MEMORY (2009)
  • Author(s): Kojima, Nobuhiko, Hiroki Yasuda, et al.
  • Organizer: The XXXVI International Congress of Physiological Sciences (IUPS2009)
  • Place of Presentation: 京都
  • Year and Date: 2009-07-28
• [Presentation] LRR膜貫通分子Lrfn2/SALM1は海馬興奮性シナプスの形態や機能調節および可塑性に関与する (2008)
  • Author(s): 守村直子、安田浩樹, 他
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2008-12-10
• [Presentation] LRR膜貫通分子Lrfn2/SALM1は海馬興奮性シナプスの形態や機能調節および可塑性に関与する (2008)
  • Author(s): 守村直子
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Book] Neuromethods : Protein Kinase Technologies (2011)
  • Author(s): H.Yasuda, H.Mukai
  • Publisher: Springer
• [Book] Neuromethods : Protein Kinase Technologies
  • Author(s): 安田浩樹、向井秀幸
  • Publisher: Springer(in press)