Role of Phosphatidylinositol Signaling in the Activation of Volume-Regulated Anion Channels

• Project/Area Number: 20500366
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Saga University
• Principal Investigator: YAMAMOTO Shintaro Saga University, 医学部, 准教授 (40336110)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 受容体 / 細胞内シグナル伝達 / イオンチャネル / 膜電流 / 細胞内情報伝達 / インスリン / イノシトールリン脂質 / 糖尿病 / 細胞容積調 / 心室筋

Research Abstract

Since it is fully unknown the mechanism in the activation of Volume-regulated anion channel (VRAC) that is an important regulator of the cell-size, I studied the relationships between VRAC activation and phosphatidylinositol signaling in mouse ventricular cells. I found the attenuation of VRAC current by PIP3 depletion in type 1 diabetic mice, the inhibition of VRAC current by a 1- adrenoceptor-mediated PIP2 depletion. In addition, preliminary data suggested that the modulation of VRAC by PIP3-PDK1-Akt signaling. These results provide novel evidences in the activation of cardiac VRAC current.

Research Products

• [Journal Article] OSU-03012, a novel celecoxib derivative, induces cell swelling and shortens action potential duration in mouse ventricular cells. (2010)
  • Author(s): Yamamoto S, Iyoda T, Kita S, Yamada T, Iwamoto T.
  • Journal Title: Biomed Res 31(6) Pages: 413-417
  • NAID: 130004470768
  • Peer Reviewed
• [Journal Article] α1-Adrenoceptor PIP2 depletion inhibits activation of volume-regulated anion channels in mouse ventricular myocytes. (2010)
  • Author(s): Ichishma K, Yamamoto S, Ehara T.
  • Journal Title: Bri J Pharmcol 61(1) Pages: 193-206
  • Peer Reviewed
• [Journal Article] 心筋細胞を中心とした細胞の容積調節に関する評価法 (2010)
  • Author(s): 山本信太郎,塩谷孝夫,頴原嗣尚,岩本隆宏
  • Journal Title: 日本薬理学雑誌 135(6) Pages: 245-249
  • NAID: 10026544275
• [Journal Article] OSU-03012, a novel celecoxib derivative, induces cell swelling and shortens_action potential duration in mouse ventricular cells (2010)
  • Author(s): Shintaro Yamamoto
  • Journal Title: Biomedical Research Volume: 31 Pages: 413-417
  • Peer Reviewed
• [Journal Article] α-Adrenoceptor-mediated PIP2 depletion inhibits activation of volume regulated anion channels in mouse ventricular myocytes. (2010)
  • Author(s): Kunihiko Ichishima
  • Journal Title: British Journal of Pharmacology Volume: 61 Pages: 193-206
  • Peer Reviewed
• [Journal Article] 心筋細胞を中心とした細胞の容積調節に関する評価法 (2010)
  • Author(s): 山本信太郎
  • Journal Title: 日本薬理学雑誌 Volume: 135 Pages: 245-249
  • NAID: 10026544275
  • Peer Reviewed
• [Journal Article] Role of Cl^- Transporters in the Cardiac Cell Volume Homeostasis. (2009)
  • Author(s): Yamamoto S, Yamada T, Iyoda T, Kita S, Iwamoto T.
  • Journal Title: Med Bull Fukuoka Univ 36 Pages: 243-255
• [Journal Article] Reduced volume-regulated outwardly rectifying anion channel activity in ventricular myocyte of type 1 diabetic mice. (2009)
  • Author(s): Yamamoto S, Ichishima K, Ehara T.
  • Journal Title: J Physiol Sci 59 Pages: 87-96
  • NAID: 10025218305
  • Peer Reviewed
• [Journal Article] Reduced Volume-Regulated Outwardly Rectifying Anion Channel Activity in Ventricular Myocyte of Type 1 Diabetic Mice (2009)
  • Author(s): Shintaro Yamamoto
  • Journal Title: The Journal of Physiological Sciences 59 Pages: 87-96
  • NAID: 10025218305
  • Peer Reviewed
• [Journal Article] Role of Cl- Channels and Transporters in the Cardiac Cell Volume Homeostasis (2009)
  • Author(s): Shintaro Yamamoto
  • Journal Title: Medical bulletin of Fukuoka University 36 Pages: 243-255
  • NAID: 110007376605
  • Peer Reviewed
• [Journal Article] Reduced volume-regulated outwardly rectifying anion channel activity in ventricular myocyte of type 1 diabetic mice. (2009)
  • Author(s): Yamamoto S, Ichishima K, Ehara T.
  • Journal Title: The Journal of Physiological Sciences 59 Pages: 313-320
  • NAID: 10025218305
  • Peer Reviewed
• [Journal Article] Regulation of volume-outwardly rectifying anion channel by phosphatidylinositol 3,4,5 triphosphate in mouse ventricular cells. (2008)
  • Author(s): Yamamoto S, Ichishima K, Ehara T.
  • Journal Title: Biomed Res 29(6) Pages: 307-307
  • Peer Reviewed
• [Journal Article] Regulation of volume-regulated outwardly rectifying anion channels by phosphatidylinositol 3, 4, 5-trisphosphate in mouse vent ricular cells. (2008)
  • Author(s): Yamamoto S, Ichishima K, Ehara T.
  • Journal Title: Biomedical Research 29 Pages: 307-315
  • NAID: 130004470730
  • Peer Reviewed
• [Presentation] Effect of OSU-03012 on the activation of volume regulated anion channels in mouse ventricular cells (2011)
  • Author(s): 山本信太郎
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-03-28
• [Presentation] OSU-03012, a novel celecoxib derivative, suppresses volume regulated a nion channels in mouse ventricular cells (2011)
  • Author(s): 山本信太郎
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-03-24
• [Presentation] Regulation of volume regulated anion channels by phosphatidylinositol signaling in heart. (2011)
  • Author(s): 市島久仁彦
  • Organizer: 第75回日本循環器学会総会・学術集会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-03-18
• [Presentation] Caveolin-3 regulates the volume-regulated anion channel in mouse ventricular cells. (2011)
  • Author(s): Yamamoto S, Kita S, Iyoda T, Yamada T, Iwamoto T.
  • Organizer: 55th Annual Meeting of Biophysical Society.
  • Place of Presentation: Baltimore MD USA
  • Year and Date: 2011-03-07
• [Presentation] Caveolin-3 regulates the volume-regulated anion channel in mouse ventricular cells (2011)
  • Author(s): 山本信太郎
  • Organizer: 55th Annual Meeting of Biophysical Society
  • Place of Presentation: Boltimore Convention Center (Baltimore, USA)
  • Year and Date: 2011-03-07
• [Presentation] Properties of volume-regulated anion channel in hypertrophic ventricular cells from caveolin-3 deficient mice. (2010)
  • Author(s): 山本信太郎, 喜多紗斗美, 伊豫田拓也, 山田敏樹, 岩本隆宏
  • Organizer: 第63回日本薬理学会西南部会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2010-11-26
• [Presentation] Properties of volume regulated anion channel in hypertrophic ventricular cells from caveolin-3 deficient mice (2010)
  • Author(s): 山本信太郎
  • Organizer: 第63回日本薬理学会西南部会
  • Place of Presentation: かごしま県民交流センター(鹿児島市)
  • Year and Date: 2010-11-26
• [Presentation] カベオリン-3による細胞容積調節性アニンチャネルの制御 (2010)
  • Author(s): 山本信太郎, 喜多紗斗美, 伊豫田拓也, 山田敏樹, 岩本隆宏
  • Organizer: 第61回西日本生理学会
  • Place of Presentation: 長崎市
  • Year and Date: 2010-10-15
• [Presentation] カベオリン-3による細胞容積調節性アニオンチャネルの制御 (2010)
  • Author(s): 山本信太郎
  • Organizer: 第61回西日本生理学会
  • Place of Presentation: 長崎大学良順会館(長崎市)
  • Year and Date: 2010-10-15
• [Presentation] 肥大心筋細胞における細胞容積調節性アニオンチャネルの電気生理学的特性 (2010)
  • Author(s): 山本信太郎, 喜多紗斗美, 伊豫田拓也, 山田敏樹, 頴原嗣尚, 岩本隆宏
  • Organizer: トラスポーター研究会第4回九州部会
  • Place of Presentation: 佐世保市
  • Year and Date: 2010-09-11
• [Presentation] 肥大心筋細胞における細胞容積調節性アニオンチャネルの電気生理学的特性 (2010)
  • Author(s): 山本信太郎
  • Organizer: 第4回トランスポーター研究会九州部会
  • Place of Presentation: ハウステンボスユトレヒト会議場(ハウステンボス町)
  • Year and Date: 2010-09-11
• [Presentation] The properties of the cardiac volume regulated outwardly rectifying anion channel in caveolin-3 deficient mice (2010)
  • Author(s): 山本信太郎
  • Organizer: 第33回心筋代謝研究会
  • Place of Presentation: キャノンマーケティングジャパン株式会社2階ホール(東京都港区)
  • Year and Date: 2010-07-04
• [Presentation] The properties of the cardiac volume-regulated outwardly rectifying anion channel in caveolin-3 deficient mice. (2010)
  • Author(s): 山本信太郎, 喜多紗斗美, 岩本隆宏
  • Organizer: 第33回心筋代謝研究会
  • Place of Presentation: 東京都港区
  • Year and Date: 2010-07-03
• [Presentation] Caveolin-3 modulates the activity of the volume regulated chloride channel in mouse ventricular cells (2010)
  • Author(s): 山本信太郎
  • Organizer: 第87回日本生理学会年会
  • Place of Presentation: 盛岡市民文化ホール(盛岡市)
  • Year and Date: 2010-05-20
• [Presentation] Caveolin-3 modulates the activity of the volume-regulated chloride channel in mouse ventricular cells. (2010)
  • Author(s): 山本信太郎, 喜多紗斗美, 伊豫田拓也, 山田敏樹, 頴原嗣尚, 岩本隆宏
  • Organizer: 第87回日本生理学会総会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-05-19
• [Presentation] カベオリン3欠損により心筋細胞の容積調節性アニオンチャネルは減弱する (2010)
  • Author(s): 山本信太郎
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-03-21
• [Presentation] 心肥大と心筋細胞容積調節 (2010)
  • Author(s): 山本信太郎
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-03-19
• [Presentation] Caveoline-3 deficiency restrains the cardiac volume-regulated outwardly rectifying anion channel. (2010)
  • Author(s): 山本信太郎, 喜多紗斗美, 伊豫田拓也, 岩本隆宏
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-03-16
• [Presentation] Cardiac hypertrophy and cell-volume regulation. (2010)
  • Author(s): 山本信太郎, 喜多紗斗美, 岩本隆宏
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-03-16
• [Presentation] PI3Kは細胞容積感受性アニオンチャネルを介して心筋細胞容積を制御する (2010)
  • Author(s): 山本信太郎, 喜多紗斗美, 伊豫田拓也, 市島久仁彦, 頴原嗣尚, 岩本隆宏
  • Organizer: 第39回日本脈管作働物質学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2010-02-05
• [Presentation] PI3Kは細胞容積感受性アニオンチャネルを介して心筋細胞容積を制御する (2010)
  • Author(s): 山本信太郎
  • Organizer: 第39回日本心脈管作働物質学会
  • Place of Presentation: 愛知県産業労働センター(名古屋市)
  • Year and Date: 2010-02-05
• [Presentation] 心筋細胞の容積調節はイノシトールリン脂質によって制御される (2009)
  • Author(s): 山本信太郎, 喜多紗斗美, 市島久仁彦, 頴原嗣尚, 岩本隆宏
  • Organizer: 第19回日本循環薬理学会
  • Place of Presentation: 京都市
  • Year and Date: 2009-11-27
• [Presentation] 心筋細胞の容積調節はイノシトールリン脂質によって制御される (2009)
  • Author(s): 山本信太郎
  • Organizer: 第19回日本循環薬理学会
  • Place of Presentation: 京都大学百周年時計台記念館(京都市)
  • Year and Date: 2009-11-27
• [Presentation] イノシトールリン脂質シグナル伝達による心筋細胞の容積調節 (2009)
  • Author(s): 山本信太郎, 喜多紗斗美, 岩本隆宏
  • Organizer: トランスポーター研究会第3回九州部会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2009-11-21
• [Presentation] イノシトールリン脂質シグナル伝達による心筋細胞の容積調節 (2009)
  • Author(s): 山本信太郎
  • Organizer: トランスポーター研究会第3回九州部会
  • Place of Presentation: 鹿児島大学鶴陵会館(鹿児島市)
  • Year and Date: 2009-11-21
• [Presentation] 細胞容積調節性アニオンチャネルのPI3Kによる機構 (2009)
  • Author(s): 山本信太郎, 市島久仁彦, 岩本隆宏, 頴原嗣尚
  • Organizer: 第60回西日本生理学会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-11-06
• [Presentation] 細胞容積調節性アニオンチャネルのPI3Kによる調節機構 (2009)
  • Author(s): 山本信太郎
  • Organizer: 第60回西日本生理学会
  • Place of Presentation: 福岡県歯科医師会館(福岡市)
  • Year and Date: 2009-11-06
• [Presentation] 細胞容積調節におけるイノシトールリン脂質による制御. (2009)
  • Author(s): 山本信太郎, 岩本隆宏
  • Organizer: 「生体膜ランスポートソームの分子構築と機能」平成21年度第1回班会議
  • Place of Presentation: 阿蘇市
  • Year and Date: 2009-08-26
• [Presentation] Reduced volume-regulated outwardly rectifying anion channel activity in ventricular myocyte of type 1 diabetic mice. (2009)
  • Author(s): 山本信太郎
  • Organizer: International Joint Symposium: Physiology of Anion Transport and Cell Volume Regulation
  • Place of Presentation: 岡崎カンファレンスセンター(岡崎市)
  • Year and Date: 2009-08-06
• [Presentation] Reduced volume-regulated outwardly rectifying anion channel activity in ventricular myocyte of type 1 diabetic mice. (2009)
  • Author(s): 山本信太郎
  • Organizer: International Joint Symposium : Physiology of Anion Transport and Cell Volume Regulation (PAT- CVR 2009).
  • Place of Presentation: Okazaki
  • Year and Date: 2009-08-03
• [Presentation] Cell-volume regulation in mammalian heart. (2009)
  • Author(s): Yamamoto S, Ehara T.
  • Organizer: International Joint Symposium : Physiology of Anion Transport and Cell Volume Regulation (PAT- CVR 2009).
  • Place of Presentation: Okazaki
  • Year and Date: 2009-08-03
• [Presentation] Cell-volume regulation in mammalian heart (2009)
  • Author(s): 山本信太郎
  • Organizer: International Joint Symposium : Physiology of Anion Transport and Cell Volume Regulation
  • Place of Presentation: 岡崎カンファレンスセンター(岡崎市)
  • Year and Date: 2009-08-03
• [Presentation] Regulation of volume-regulated outwardly rectifying anion channels by phosphatidylinositol 3. 4. 5-trisphosphate in mouse ventricular cells. (2009)
  • Author(s): Yamamoto S, Ichishima K, Ehara T.
  • Organizer: XXXVI International Congress of Physiological Sciences (IUPS 2009).
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-27
• [Presentation] Regulation of volume-regulated outwardly rectifying anion channels by phosphatidylinositol 3,4,5-trisphosphate in mouse ventricular cells (2009)
  • Author(s): 山本信太郎
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2009-07-27
• [Presentation] The Regulation of Volume-Regulated Outwardly Rectifying Anion Channels by Membrane Phosphatidylinositides in Mouse Ventricular Cells. (2009)
  • Author(s): Yamamoto S, Ichishima K, Ehara T.
  • Organizer: 53th Annual Meeting of Biophysical Society.
  • Place of Presentation: Boston MA USA
  • Year and Date: 2009-02-28
• [Presentation] The regulation of volume-regulated outwardly rectifying anion channels by membrane phosphatidylinositides in mouse ventricular cells. (2009)
  • Author(s): Yamamoto S, Ichishima K, Ehara T
  • Organizer: 第53回国際生物物理学会
  • Place of Presentation: 米国ボストン市
• [Presentation] マウス心室筋における細胞容積調節性アニオンチャネルのイノシトールリン脂質による調節 (2008)
  • Author(s): 山本信太郎
  • Organizer: トランスポターワークシップIN福岡
  • Place of Presentation: 福岡市
  • Year and Date: 2008-11-02
• [Presentation] α受容体刺激によるマウス心筋型I_<Cl.swell>の抑制PIP3減少が関与する (2008)
  • Author(s): 市島久仁彦, 山本信太郎, 頴原嗣尚
  • Organizer: 第59回西日本生理学会
  • Place of Presentation: 熊本市
  • Year and Date: 2008-10-03
• [Presentation] マウス心室筋における細胞容積調節性アニオンチャネルのイノシトールリン脂質による調節 (2008)
  • Author(s): 山本信太郎
  • Organizer: 「生体膜ランスポートソームの分子構築と体機能」平成20年度第1回班会議
  • Place of Presentation: 淡路市
  • Year and Date: 2008-09-24
• [Presentation] α受容体刺激によるマウス心筋型IC1. swellの抑制にPIP3減少が関与する. (2008)
  • Author(s): 市島 久仁彦, 山本信太郎, 頴原嗣尚
  • Organizer: 第59回西日本生理学会
  • Place of Presentation: 熊本県熊本市
• [Presentation] Effect of OSU activation of volume-channels in mouse ventricular cells
  • Author(s): Yamamoto S, Iyoda T, Kita S Iwamoto T.
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: (東北地方太平洋沖地震のため中止。抄録集上での開催)
• [Presentation] OSU-03012, a novel celecoxib derivative, suppresses volume-regulated anion channels in mouse ventricular cells.
  • Author(s): Yamamoto S, Iyoda T, Kita S Iwamoto T.
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: (東北地方太平洋沖地震のため中止。抄録集上での開催)
• [Presentation] Regulation of volume-regulated anion channels by phosphatidylinositol signaling in heart.
  • Author(s): Ichishima K, Yamamoto S, Ehara T.
  • Organizer: 第75回日本循環器学会学術集会
  • Place of Presentation: (東北地方太平洋沖地震のため中止。抄録集上での開催)
• [Book] 新しい医学教育の流れ'10春 (2010)
  • Author(s): 山本信太郎
  • Total Pages: 5
  • Publisher: 岐阜大学医学教育開発センター
• [Remarks]
  • URL: http://resweb2.jhk.adm.fukuoka-u.ac.jp/FukuokaUnivHtml/info/5169/R107J.html
• [Remarks]
  • URL: http://resweb2.jhk.adm.fukuoka-u.ac.jp/FukuokaUnivHtml/info/5169/R110J.html