Functional analysis of muscle regeneration using gene-targeting and transgenic technologies

• Project/Area Number: 20500367
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kitasato University
• Principal Investigator: HANAOKA Kazunori Kitasato University, 理学部, 教授 (40189577)
• Co-Investigator(Kenkyū-buntansha): WATANABE Daisuke 北里大学, 理学部, 講師 (00260175) ; KITAMOTO Takero 北里大学, 理学部, 助教 (20453516)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 筋再生 / サテライト細胞 / Lbx1 / Notch3 / マウス / Dp71 / 筋肉 / 幹細胞 / 筋ジストロフィー

Research Abstract

The results of present study demonstrate Notch3 as an important regulator for muscle repair. The data indicate Notch3 negatively regulate both proliferation and self-renewal of activated satellite cells after muscle injury. These results provide insight into the mechanisms of self-renewal and homeostatic regulation of satellite cells during muscle regeneration.

Research Products

• [Journal Article] Spatially and temporally regulated expression of specific heparan sulfate epitopes the developing mouse olfactory system (2010)
  • Author(s): Takatoh J, Hanaoka K
  • Journal Title: Dev Growth Diff 52 Pages: 169-80
  • NAID: 10027563694
• [Journal Article] Notch3 null mutation in mice causes muscle hyperplasia repetitive muscle regeneration (2010)
  • Author(s): Kitamoto T., Hanaoka K.
  • Journal Title: SYStem Cells 28 Pages: 2205-2216
• [Journal Article] Spatially and temporally regulated expression of specific heparan sulfate epitopes in the developing mouse olfactory system (2010)
  • Author(s): Takatoh J, Hanaoka K
  • Journal Title: Dev Growth Diff Volume: 52 Pages: 169-180
  • NAID: 10027563694
  • Peer Reviewed
• [Journal Article] Notch3 null mutation in mice causes muscle hyperplasia by repetitive muscle regeneration (2010)
  • Author(s): Kitamoto T, Hanaoka K.
  • Journal Title: Stem Cells Volume: 28 Pages: 2205-2216
  • Peer Reviewed
• [Journal Article] Generation of a conditional null allele of Lbx1 (2010)
  • Author(s): Watanabe S, Matsushita S, Hayasaka M., Hanaoka K
  • Journal Title: Genesis Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Spatially and temporally regulated expression of specific heparinsulfate epitopes in the developing mouse olfactory system (2010)
  • Author(s): Takatoh J, Hanaoka K
  • Journal Title: Dev Growth Differ 52 Pages: 169-180
  • Peer Reviewed
• [Journal Article] K.Hanaoka Y. Targeted deletion of the tybe IIb Na+-dependent Pi-co-transporter NaPi-IIb results in early embryonic lethality Biochem. (2009)
  • Author(s): Y. Shibasaki,N. Etoh, M. Hayasaka, M.Takahashi,M.Kakitani,T.Yamashita, Tomizuka
  • Journal Title: Biophys.ResCommuni 381 Pages: 482-486
• [Journal Article] Targeted Deletion of the Na+-dependent Pi-co-transporter, NaPi-IIb, Results in Early Embryonic Lethality (2009)
  • Author(s): Y Shibasaki, N Etoh, M Hayasaka, M Takahashi, M Kakitani, TYamashita, K Tomizuka, K Hanaoka
  • Journal Title: Biochem. Biophys. Res. Communi. 381 Pages: 482-486
  • Peer Reviewed
• [Journal Article] Involvement of Short Dystrophin Isoform Dp71 in Organization of Mouse Vomeronasal Nerve (2008)
  • Author(s): Takatoh J, Kudo H,Kondo S, Hanaoka K.
  • Journal Title: Exp.Neurol 213(1) Pages: 36-47
• [Journal Article] Skeletogenesis in Xenopus tropicalis Characteristic bone development in an anuran amphibian (2008)
  • Author(s): S..Miura, K.Hanaoka, S.Togashi
  • Journal Title: Bone. 43,(5) Pages: 901-909
• [Journal Article] Involvement of Short Dystrophin Isoform Dp71 in Organization of Mouse Vomeronasal Nerve. (2008)
  • Author(s): Takatoh J, Kudo H, Kondo S, Hanaoka K.
  • Journal Title: Exp. Neurol 213 Pages: 36-47
  • Peer Reviewed
• [Journal Article] Skeletogenesis in Xenopus tropicalis : Characteristic bone development in an anuran amphibian (2008)
  • Author(s): S. . Miura, K. Hanaoka, S. Togashi
  • Journal Title: Bone. 43 Pages: 901-909
  • Peer Reviewed
• [Journal Article] Generation of a conditional null allele of Lbx1
  • Author(s): Watanabe S, Matsushita S, Hayasaka M., Hanaoka K.
  • Journal Title: Genesis In press
• [Presentation] Generation of mice with conditional null allele of Lbx1 gene (2010)
  • Author(s): 松下祥子, ら4名
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 機能的なinv遺伝子に対するRNAiノックダウンマウスの作成とその表現型解析 (2010)
  • Author(s): 塩崎弥生, ら5名
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Notch3 null mutation in mice causes muscle hyperplasia by repetitive muscle regeneration (2010)
  • Author(s): 北本武郎, ら2名
  • Organizer: 日本分子生物学会
  • Place of Presentation: 紳戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] Notch3 nullmutation in mice causes muscle hyperplasia by repetitive muscleregeneration (2010)
  • Author(s): 北本武郎, 花岡和則
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 渡邉機能的なinv遺伝子に対するRNAiノックダウンマウスの作成とその表現型解析 (2010)
  • Author(s): 塩崎、伏見、来栖、花岡
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Generation of mice withconditional null allele of Lbx1 gene (2010)
  • Author(s): Watanabe S, Matsushita S, Hayasaka M, Hanaoka K
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Notch3 regulates satellite cell proliferation in adult skeletal muscle (2009)
  • Author(s): 北本武郎, 花岡和則
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Notch3regulates satellite cell proliferation in adult skeletal muscle (2009)
  • Author(s): 北本武郎, 花岡和則
  • Organizer: 第32回日本分子生
  • Place of Presentation: 横浜
• [Presentation] Specific expressions of mouse denovo methyltransferases during stem cell differentiation (2009)
  • Author(s): 梅原芳惠, 坂本祐基, 花岡和則, 渡邉大介
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Expression dynamics of the imprinted transgenes during mouse germcell development (2009)
  • Author(s): 内山孝司、渡邉大介、早坂美智子、花岡和則
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Remarks] ホームページ