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Development of the analysis system for the function of glycosylation-related genes involved in embryonic lethality

Research Project

Project/Area Number 20500376
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionNagoya City University

Principal Investigator

MIYOSHI Ichiro  Nagoya City University, 大学院・医学研究科, 教授 (10183972)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords糖鎖 / 遺伝子 / 発生分化 / 実験動物学 / セラミド細胞内選択輸送タンパク質
Research Abstract

We attempted to establish the analysis system for the function of Ugcg(UDP-Glucose Ceramide Glucosyltransferase) gene on the basis of the quantitative difference, from complete deficiency to overexpression, in the expression of homologue gene introduced into Ugcg-deficient ES cell on both alleles. However, stably transfected clone was note obtained. We generated Ceramide Transfer Protein (Cert) conditional knockout mice deficient in exon5 by using the Cre-LoxP system. As Cert proved to be an essential gene for mouse development and embryonic survival, this mice is a new model useful for the study of the function of glycosylation-related genes involved in embryonic lethality.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (20 results)

All 2011 2010 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (3 results) Book (3 results) Remarks (2 results)

  • [Journal Article] Impairment of neuropsychological behaviors in ganglioside GM3-knockout mice.2011

    • Author(s)
      Niimi K, Miyoshi I, ら
    • Journal Title

      Biochem Biophys Res Commun 406

      Pages: 524-528

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impairment of neuropsychological behaviors in ganglioside GM3-knockout mice.2011

    • Author(s)
      Niimi K, ら
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 406 Pages: 524-8

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cooperative functions of Chk1 and Chk2 reduce tumour susceptibility in vivo.2010

    • Author(s)
      Niida H, Miyoshi I, ら
    • Journal Title

      EMBO J 29

      Pages: 3558-3570

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subventricular zone-derived neural progenitor cells migrate along a blood vessel scaffold toward the post-stroke striatum.2010

    • Author(s)
      Kojima T, Miyoshi I, ら
    • Journal Title

      Stem Cells 28

      Pages: 545-554

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] モデル動物の開発とその応用2010

    • Author(s)
      三好一郎
    • Journal Title

      Nagoya Med J 51(1)

      Pages: 55-61

    • Related Report
      2010 Final Research Report
  • [Journal Article] Subventricular zone-derived neural progenitor cells migrate along a blood vessel scaffold toward the post-stroke striatum.2010

    • Author(s)
      Kojima T, ら
    • Journal Title

      Stem Cells

      Volume: 28 Pages: 545-45

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cooperative functions of Chk1 and Chk2 reduce tumour susceptibility in vivo.2010

    • Author(s)
      Niida H, ら
    • Journal Title

      EMBO J

      Volume: 29 Pages: 3558-70

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Subventricular zone-derived neural progenitor cells migrate along a blood vessel scaffold toward the post-stroke striatum2010

    • Author(s)
      Kojima T, ら
    • Journal Title

      Stem Cells 28

      Pages: 545-54

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rap signaling is crucial for the competence of IL-7 response and the development of B-lineage cells.2009

    • Author(s)
      Katayama Y, Miyoshi I, ら
    • Journal Title

      Blood 114

      Pages: 1768-1775

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rap signaling is crucial for the competence of IL-7 response and the development of B-lineage cells2009

    • Author(s)
      Katayama Y, ら
    • Journal Title

      Blood 114

      Pages: 1768-75

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zebrafish with antisense-knockdown of cardiac troponin C as a model of hereditary dilated cardiomyopathy2009

    • Author(s)
      Ohte N, ら
    • Journal Title

      Circ J 73

      Pages: 1595-6

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modified sympathetic nerve system activity with over-expression of the voltage-dependent calcium channel β3 subunit.2008

    • Author(s)
      Murakami M
    • Journal Title

      J Biol Chem 367

      Pages: 24554-60

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 動物実験に関わるカルタヘナ法について2010

    • Author(s)
      三好一郎
    • Organizer
      公私立大学実験動物施設協議会第16回シンポジウム
    • Place of Presentation
      ルビノ京都堀川(特別講演)
    • Year and Date
      2010-06-04
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] セラミド細胞内選別輸送タンパク質(Ceramide transfer protein ; Cert)欠損マウスの作製2009

    • Author(s)
      久野雅広, 三好一郎, ら
    • Organizer
      東海実験動物研究会
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2009-09-12
    • Related Report
      2010 Final Research Report
  • [Presentation] セラミド細胞内選別輸送タンパク質(Ceramide transfer protein ; Cert)欠損マウスの作製2009

    • Author(s)
      久野雅広, ら
    • Organizer
      東海実験動物研究会
    • Place of Presentation
      名古屋市立大学(名古屋市)
    • Year and Date
      2009-09-12
    • Related Report
      2009 Annual Research Report
  • [Book] 発生工学(現代実験動物学)(笠井憲雪,吉川泰弘,安居院高志(編))2009

    • Author(s)
      三好一郎
    • Related Report
      2010 Final Research Report
  • [Book] 発生工学. 現代実験動物学. 168-181(笠井憲雪, 吉川泰弘, 安居院高志(編))2009

    • Author(s)
      三好一郎
    • Total Pages
      219
    • Publisher
      朝倉書店
    • Related Report
      2009 Annual Research Report
  • [Book] 9. 発生工学. 笠井憲雪, 吉川泰弘, 安居院高志(編)現代実験動物学168-181.2009

    • Author(s)
      三好一郎
    • Total Pages
      219
    • Publisher
      朝倉書店
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.med.nagoya-cu.ac.jp/animal.dir/animalweb/dcem/index_ncu.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.med.nagoya-cu.ac.jp/animal.dir/animalweb/dcem/index_ncu.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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