Basic study on follicular oocyte maturation by in vitro culture and production of offspring from embryos derived from IVM-maturation oocytes
| Project/Area Number |
20500379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Nihon University |
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Principal Investigator |
SATO Kahei Nihon University, 生物資源科学部, 教授 (20059778)
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Naoto 日本大学, 生物資源科学部, 講師 (70297795)
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Nao 聖マリアンナ医科大学, 医学部, 講師 (90246356)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 卵胞卵子成熟 / 共培養 / リコンビナントFSH / IVM / vitrification / recombinant FSH / 凍結卵巣由来卵胞培養 / 体外成熟卵子 / COCs / ICSI / 凍結卵巣 / 卵丘細胞 / ガラス化法 / 卵胞の体外培養 / 卵胞卵子の体外成熟 |
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| Research Abstract |
In this study, using recombinant FSH (recFSH) the in vitro maturation (IVM) of mouse follicular oocytes was investigated. Using recFSH IVM showed high maturation rate of the oocytes obtained from fresh or frozen/thawed ovarian tissues. Fertilization of the mature oocytes obtained showed high rate and its embryos developed into offspring by transfer into foster mothers. Co-culture of follicular oocytes with mouse fibroblasts also resulted in constant maturation rates compared with control (in vivo maturation oocytes).
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Report
(4 results)
Research Products
(9 results)
