A high-speed congenic strategy using first-wave male germ cells

• Project/Area Number: 20500383
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: OGONUKI Narumi The Institute of Physical and Chemical Research, 遺伝工学基盤技術室, 専任技師 (40373287)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: マウス / 実験動物 / 発生工学 / 顕微授精

Research Abstract

To establish high-speed congenic system, microinsemination with round spermatid which retrieved from immature mouse selected by marker-assisted screening system was tried. Nine congenic strains were produced by this method for 106 and 190 days of N3 to N5 generation. The generation turnover time in mice can be shorted to about 40 days by using the first wave round spermatids as male gametes. This method can establish congenic strain the period below about half of conventional method.

Research Products

• [Journal Article] In vitro production of functional sperm in cultured neonatal mouse testes. (2011)
  • Author(s): Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T
  • Journal Title: Nature 471 Pages: 504-507
  • Peer Reviewed
• [Journal Article] Production of mouse embryonic stem cell lines from maturing oocytes by direct conversion of meiosis into mitosis. (2011)
  • Author(s): Fulka H, Hirose M, Inoue K, Ogonuki N, Wakisaka N, Matoba S, Ogura A, Mosko T, Kott T, Fulka J.Jr.
  • Journal Title: Stem Cells 29(3) Pages: 517-27
  • Peer Reviewed
• [Journal Article] Serum- and Feeder-Free Culture of Mouse Germline Stem Cells. (2011)
  • Author(s): Matoba S, Ogura A, Kanatsu-Shinohara M, Inoue K, Ogonuki N, Morimoto H, Ogura A, Shinohara T
  • Journal Title: Biol.Reprod. 84 Pages: 97-105
  • Peer Reviewed
• [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer. (2011)
  • Author(s): Inoue K, et.al.
  • Journal Title: Science Volume: 330 Pages: 496-499
  • Peer Reviewed
• [Journal Article] Serum- and Feeder-Free Culture of Mouse Germline Stem Cells. (2011)
  • Author(s): Kanatsu-Shinohara M, et.al.
  • Journal Title: Biol.Reprod. Volume: 84 Pages: 97-105
  • Peer Reviewed
• [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer. (2010)
  • Author(s): Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A
  • Journal Title: Science 330 Pages: 496-499
  • Peer Reviewed
• [Journal Article] The effect on intracytoplasmic sperm injection outcome of genotype, male germ cell stage and freeze-thawing in mice. (2010)
  • Author(s): Ogonuki N, Mori M, Shinmen A, Inoue K, Mochida K, Ohta A, Ogura A
  • Journal Title: PLoS ONE 5(6)
  • Peer Reviewed
• [Journal Article] Production of mouse embryonic stem cell lines from maturing oocytes by direct conversion of meiosis into mitosis. (2010)
  • Author(s): Fulka H, et.al.
  • Journal Title: Stem Cells Volume: (In press)
  • Peer Reviewed
• [Journal Article] The effect on intracytoplasmic sperm injection outcome of genotype, male germ cell stage and freeze-thawing in mice. (2010)
  • Author(s): Ogonuki N, et.al.
  • Journal Title: PLoS ONE Volume: 5
  • Peer Reviewed
• [Journal Article] Genetic influences in mouse spermatogonial stem cell self-renewal. (2009)
  • Author(s): Kanatsu-Shinohara M, Ogonuki N, Miki H, Inoue K, Morimoto H, Ogura A, Shinohara T
  • Journal Title: J Reprod.Dev 56 Pages: 145-153
  • NAID: 10026323942
  • Peer Reviewed
• [Journal Article] Sex-reversed somatic cell cloning in the mouse. (2009)
  • Author(s): Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, Ogura A
  • Journal Title: J Reprod Dev 55 Pages: 566-569
  • NAID: 10025616710
  • Peer Reviewed
• [Journal Article] Embryonic Rather than Extraembryonic Tissues Have More Impact on the Development of Placental Hyperplasia in Cloned Mice. (2009)
  • Author(s): Miki H, Wakisaka N, Inoue K, Ogonuki N, Mori M, Kim JM, Ohta A, Ogura A
  • Journal Title: Placenta 30 Pages: 543-546
  • Peer Reviewed
• [Journal Article] A high-speed congenic strategy using first-wave male germ cells. (2009)
  • Author(s): Ogonuki N, Inoue K, Hirose M, Miura I, Mochida K, Sato T, Mise N, Mekada K, Yoshiki A, Abe K, Kurihara H, Wakana S, Ogura A
  • Journal Title: PLoS One 4(3)
  • Peer Reviewed
• [Journal Article] Heritable Imprinting Defect Caused by Epigenetic Abnormalities in Mouse Spermatogonial Stem Cells. (2009)
  • Author(s): Lee J, Kanatsu-Shinohara M, Ogonuki N, Miki H, Inoue K, Morimoto T, Morimoto H, Ogura A, Shinohara T
  • Journal Title: Biol Reprod 80 Pages: 518-527
  • Peer Reviewed
• [Journal Article] Efficient production of androgenetic embryos by round spermatid injection. (2009)
  • Author(s): Miki H, Hirose M, Ogonuki N, Inoue K, Kezuka F, Honda A, Mekada K, Hanaki K, Iwafune H, Yoshiki A, Ishino F, Ogura A
  • Journal Title: Genesis 47 Pages: 155-60
  • Peer Reviewed
• [Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries. (2009)
  • Author(s): Honda A, Hirose M, Inoue K, Hiura H, Miki H, Ogonuki N, Sugimoto M, Abe K, Kanatsu-Shinohara M, Kono T, Shinohara T, Ogura A
  • Journal Title: Int J Dev Biol 53 Pages: 605-613
  • Peer Reviewed
• [Journal Article] Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes. (2009)
  • Author(s): Tachibana M, Terada Y, Ogonuki N, Ugajin T, Ogura A, Murakami T, Yaegashi N, Okamura K
  • Journal Title: Mol Reprod Dev 76 Pages: 270-277
  • Peer Reviewed
• [Journal Article] Sex-reversed somatic cell cloning in the mouse (2009)
  • Author(s): Inoue, K, et.al.
  • Journal Title: J Reprod Dev 55 Pages: 566-569
  • Peer Reviewed
• [Journal Article] Genetic Influences in Mouse Spermatogonial Stem Cell Self-Renewal (2009)
  • Author(s): Kanatsu-Shinohara, M, et.al
  • Journal Title: J Reprod Dev Nov 19
  • Peer Reviewed
• [Journal Article] Heritable imprinting defect caused by epigenetic abnormalities in mouse spermatogonial stem cells (2009)
  • Author(s): Lee, J, et.al.
  • Journal Title: Biol Reprod 80 Pages: 518-527
  • Peer Reviewed
• [Journal Article] Embryonic rather than extraembryonic tissues have more impact on the development of placental hyperplasia in cloned mice (2009)
  • Author(s): Miki, H, et.al.
  • Journal Title: Placenta 30 Pages: 543-546
  • Peer Reviewed
• [Journal Article] The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass (2009)
  • Author(s): Nishioka, N, et.al.
  • Journal Title: Dev Cell 16 Pages: 398-410
  • Peer Reviewed
• [Journal Article] A high-speed congenic strategy using first-wave male germ cells (2009)
  • Author(s): Ogonuki, N, et.al.
  • Journal Title: PLoS One 4
  • Peer Reviewed
• [Journal Article] Stable ES cell lines in rabbits-potential small animal models for human ES cell research. (2008)
  • Author(s): Honda A, Hirose M, Inoue K, Ogonuki N, Miki H, Shimozawa N, Hatori M, Shimizu N, Murata T, Hirose H, Katayama K, Wakisaka N, Miyoshi H, Yokoyama KK, Sankai T, Ogura A
  • Journal Title: Reprod Biomed Online 17 Pages: 706-715
  • Peer Reviewed
• [Journal Article] Ultrastructure of placental hyperplasia in mice : Comparison of placental phenotypes with three different etiologies. (2008)
  • Author(s): Wakisaka N, Inoue K, Ogonuki N, Miki H, Sekita Y, Hanaki K, Akatsuka K, Kaneko-Ishino T, Ishino F, Ogura A
  • Journal Title: Placenta 29 Pages: 753-759
  • NAID: 130007022881
  • Peer Reviewed
• [Journal Article] Differential effects of Akt signaling on two types of nuclear reprogramming. (2008)
  • Author(s): Nakamura T, Inoue K, Ogawa S, Umehara H, Ogonuki N, Miki H, Kimura T, Ogura A, Nakano T
  • Journal Title: Genes Cells 13 Pages: 1269-1277
  • Peer Reviewed
• [Journal Article] Birth of normal offspring from mouse eggs activated by a phospholipase Cζ protein lacking three EF-hand domains. (2008)
  • Author(s): Nakanishi T, Ishibashi N, Kubota H, Inoue K, Ogonuki N, Ogura A, Kashiwabara S, Baba T
  • Journal Title: J Reprod Dev 54 Pages: 244-249
  • NAID: 10024289241
  • Peer Reviewed
• [Journal Article] Pluripotency of a single spermatogonial stem cell in mice. (2008)
  • Author(s): Kanatsu-Shinohara M, Lee J, Inoue K, Ogonuki N, Miki H, Toyokuni S, Ikawa M, Nakamura T, Ogura A, Shinohara T
  • Journal Title: Biol Reprod 78 Pages: 681-687
  • Peer Reviewed
• [Journal Article] Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes (2008)
  • Author(s): Tachibana M, et. al.
  • Journal Title: Molecular reproduction and development 76 Pages: 270-277
  • Peer Reviewed
• [Journal Article] Ultrastructure of placental hyperplasia in mice : Comparison of placental phenotypes with three different etiologies (2008)
  • Author(s): Wakisaka N, et. al.
  • Journal Title: Placenta 29 Pages: 753-759
  • Peer Reviewed
• [Journal Article] Birth of normal offspring from mouse eggs activated by a phospholipase Czeta protein lacking three EF-hand domains (2008)
  • Author(s): Nakanishi T, et. al.
  • Journal Title: The Journal of reproduction and development 54 Pages: 244-249
  • Peer Reviewed
• [Journal Article] Pluripotency of a single spermatogonial stem cell in mice (2008)
  • Author(s): Kanatsu-Shinohara M, et. al.
  • Journal Title: Biology of Reproduction 78 Pages: 681-687
  • Peer Reviewed
• [Journal Article] Stable ES cell lines in rabbits-potential small animal models for human ES cell research (2008)
  • Author(s): Honda A, et. al.
  • Journal Title: Reproductive Biomedicine Online 17 Pages: 706-715
  • Peer Reviewed
• [Journal Article] Birth of normal mice following round spermatid injection without artificial oocyte activation.
  • Author(s): Ogonuki N, Inoue K, Ogura A
  • Journal Title: J Reprod Dev (in press)
  • NAID: 10029593452
  • Peer Reviewed
• [Journal Article] Efficient production of androgenetic embryos by round spermatid injection
  • Author(s): Miki H, et. al.
  • Journal Title: Genesis
  • Peer Reviewed
• [Journal Article] Differential effects of Akt signaling on two types of nuclear reprogramming
  • Author(s): Nakamura T, et. al.
  • Journal Title: Genes Cells
  • Peer Reviewed
• [Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries
  • Author(s): Honda A, et. al.
  • Journal Title: The International journal of developmental biology
  • Peer Reviewed
• [Presentation] マウス顕微授精における系統、精子成熟度、凍結保存の影響に関する統計学的解析 (2010)
  • Author(s): Ogonuki N, et.al.
  • Organizer: The 4th AFLAS Congress Meeting
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-11-09
• [Presentation] Factors affecting the outcome of mouse ICSI : Factorial analysis by genotypes stage of male germ cells and their freezing treatment (2010)
  • Author(s): Ogonuki N, et.al.
  • Organizer: 11th International Symposium on Spermatology
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 2010-06-24
• [Presentation] マウス顕微授精における系統、精子成熟度、凍結保存の影響に関する統計学的解析 (2010)
  • Author(s): 越後貫成美, ほか
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-12
• [Presentation] Factors affecting the outcome of mouse ICSI : Factorial analysis by genotypes, stage of male germ cells, and freeze-thawing treatment. (2010)
  • Author(s): 越後貫成美, et al
  • Organizer: The 4^<th> AFLAS Congress Meeting.
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Factors affecting the outcome of mouse ICSI : Factorial analysis by genotypes, stage of male germ cells, and their freezing treatment. (2010)
  • Author(s): 越後貫成美, et al
  • Organizer: 11^<th> International Symposium on Spermatology.
  • Place of Presentation: Okinawa, Japan
• [Presentation] マウス顕微授精における系統、精子成熟度、凍結保存の影響に関する統計学的解析 (2010)
  • Author(s): 越後貫成美, ほか
  • Organizer: 第57回日本実験動物学会
  • Place of Presentation: 京都
• [Presentation] 実験動物における顕微授精の応用 (2009)
  • Author(s): 越後貫成美
  • Organizer: 第2回疾患モデルシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-11-17
• [Presentation] 実験動物における顕微授精の応用 (2009)
  • Author(s): 越後貫成美
  • Organizer: 第2疾患モデルシンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2009-11-17
• [Presentation] 人為的卵子活性化処理なしでの凍結円形精子細胞の顕微授精によるマウス産仔の作出 (2009)
  • Author(s): 越後貫成美, ほか
  • Organizer: 第102回日本繁殖生物学会
  • Place of Presentation: 奈良
• [Presentation] マウス顕微授精効率への系統、精子成熟度、および精子(細胞)凍結の影響に関する統計学的解析 (2009)
  • Author(s): 森真菜実、越後貫成美, ほか
  • Organizer: 第102回日本繁殖生物学会
  • Place of Presentation: 奈良
• [Presentation] 顕微授精技術を利用したマウス超迅速コンジェニック化技術の確立 (2009)
  • Author(s): 越後貫成美, ほか
  • Organizer: 第56回日本実験動物学会
  • Place of Presentation: 大宮
• [Presentation] 理研BRC日本マウスクリニックIV:安定的・効率的な検査個体の供給システムの開発 (2009)
  • Author(s): 三浦郁生, ほか
  • Organizer: 第56回日本実験動物学会
  • Place of Presentation: (大宮ソニックシティ)さいたま市
• [Presentation] 顕微授精技術を利用したマウス超迅速コンジェニック化技術の確立 (2009)
  • Author(s): 越後貫成美, ほか
  • Organizer: 第56回日本実験動物学会
  • Place of Presentation: (大宮ソニックシティ)さいたま市
• [Presentation] 実験動物を用いた顕微授精技術の応用 (2009)
  • Author(s): 越後貫成美
  • Organizer: 第56回日本実験動物学会
  • Place of Presentation: (大宮ソニックシティ)さいたま市
• [Presentation] 人為的活性化処理なしでの凍結円形精子細胞の顕微注入によるマウス産仔の作出 (2009)
  • Author(s): 越後貫成美, ほか
  • Organizer: 第102日本繁殖生物学会
  • Place of Presentation: (近畿大学)奈良
• [Presentation] マウス顕微授精効率への系統、精子成熟度、および精子(細胞)凍結影響に関する統計学的解析 (2009)
  • Author(s): 森真菜実
  • Organizer: 第102日本繁殖生物学会
  • Place of Presentation: (近畿大学)奈良
• [Presentation] 実験動物の未熟精子を用いた受精について (2008)
  • Author(s): 越後貫成美
  • Organizer: 第4回東京受精・胚移植研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-27
• [Presentation] 顕微授精技術を利用した超迅速コンジェニック化技術の確立 (2008)
  • Author(s): 越後貫成美, ほか
  • Organizer: 第101回日本繁殖生物学会
  • Place of Presentation: 福岡
• [Presentation] 顕微授精技術を利用した超迅速コンジェニック化技術の確立 (2008)
  • Author(s): 越後貫成美, ほか
  • Organizer: 第22回モロシヌス研究会
  • Place of Presentation: 東京
• [Presentation] High-speed congenic using immature male germ cells (2008)
  • Author(s): Ogonuki N, et. al.
  • Organizer: The 5^<th> Annual Conference of the Asian Reproductive Biotechnology Society
  • Place of Presentation: Kunming, Unnan, China
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