Cardiac action potential and Ca2+ ion simultaneous dual measurement and temporal and spatial high resolution optical mapping system
| Project/Area Number |
20500387
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAKUMA Ichiro The University of Tokyo, 大学院・工学系研究科, 教授 (50178597)
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| Co-Investigator(Renkei-kenkyūsha) |
KODAMA Itsuo 名古屋大学, 環境医学研究所, 教授 (30124720)
HONJO Haruo 名古屋大学, 環境医学研究所, 准教授 (70262912)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 生体情報 / 計測 / 生体医工学 / 不整脈 / 膜電位感受性色素 / 細胞内カルシウム濃度 / 蛍光計測 / マッピング / 生物・生体工学 / 循環器・高血圧 / 生体生命情報学 / 可視化 |
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| Research Abstract |
We developed optical mapping system which records two signals simultaneously using single camera by taking each frame synchronized with filter switching. We designed the optical lens system to concentrate fluorescent light at filter position and form image on the CMOS sensor, additionally a custom filter holder for long-pass filter and band-pass filter which is rotated by a motor. Using this system, we observed Vm and Ca2+ dynamics in isolated rabbit heart. Firstly in the first step of basic study, there ware many difficult about the optical design of measuring system. Then we developed another solution for exquisite Vm and Ca dual mapping system that was constructed by 2 camera, dichroic mirror and Mutual Information based registration software both.
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Report
(4 results)
Research Products
(33 results)
