| Project/Area Number |
20500402
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki University of Medical Welfare |
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Principal Investigator |
KATAOKA Noriyuki Kawasaki University of Medical Welfare, 医療技術学部, 准教授 (20250681)
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| Co-Investigator(Kenkyū-buntansha) |
TACHIBANA Hiroyuki 川崎医療福祉大学, 医療技術学部, 講師 (00241216)
KAJIYA Fumihiko 川崎医療福祉大学, 医療技術学部, 教授 (70029114)
HIRAMATSU Osamu 川崎医療福祉大学, 医療技術学部, 准教授 (50208849)
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| Co-Investigator(Renkei-kenkyūsha) |
HASHIMOTO Ken 川崎医科大学, 医学部, 講師 (80341080)
TSUJIOKA Katsuhiko 川崎医科大学, 医学部, 元教授 (30163801)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 白血球 / 内皮細胞 / 情報伝達 / ナノバイオロジー・ナノメディスン / 動脈硬化 / アクチンフィラメト / ナノバイオロジー / ナノメディスン |
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| Research Abstract |
It is well known that one of the critical events in early atherosclerosis is the recruitment of blood monocytes to proatherogenic vascular regions and subsequent transmigration across vascular endothelial cells. It is still unknown how monocytes infiltrate into between and underneath EC monolayer. To elucidate this phenomenon, direct observation of cytoskeletal structure and/or signaling molecules in migrating monocytes under the controlled culture condition is necessary. In this study, we have established the observation system of migration of monocytic cell line induced by chemoattractant from micropipette. We have estimated the migration of HL-60 and THP-1 seeded on gelatin or fibronectin, to fMLP or MCP-1. In our experiments, THP-1 cells seeded on fibronectin actively migrated to the tip of micropipette. fMLP was more effective than MCP-1. In addition, we have found that PECAM-1 in EC is one of the key molecules that controls the leukocytes trans-endothelial migration and SHP-2 exists in the upstream of signaling transduction.
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