PEG-grafted linear cationic polymer for tumor accumulation and cell penetration

• Project/Area Number: 20500410
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyushu University
• Principal Investigator: KANO Arihiro Kyushu University, 先導物質化学研究所, 准教授 (30403950)
• Co-Investigator(Renkei-kenkyūsha): MARUYAMA Atsushi 九州大学, 先導物質化学研究所, 教授 (40190566)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 薬物伝達システム / 核酸医薬 / カチオン性高分子 / 腫瘍集積性 / 高分子構造・物性 / 生体材料 / 癌 / 光線力学療法 / PEG / PLL / 共重合体

Research Abstract

We previously showed that polyethyleneglycol-grafted poly-L-lysine (PLL-g-PEG) exerts long lifetime of nucleic acids medicine, such as siRNA, in blood circulation. It is conceivable that PLL-g-PEG is useful as a drug delivery carrier for tumor treatment. In this study, we examined the effects of PEG grafting ratio and the length of the PEG chain and PLL backbone on the stability of siRNA/PLL-g-PEG complex and the tumor accumulation. As the results, it was revealed that the higher PEG-grafted PLL, more than 30%, is efficiently accumulated in tumor tissues and even the affinity to the siRNA is augmented.

Research Products

• [Journal Article] Grafting of poly (ethylene glycol) to poly-lysine augments its lifetime in blood circulation and accumulation in tumors without loss of the ability to associate with siRNA. (2011)
  • Author(s): Kano, A., Moriyama, K., Yamano, T., Nakamura, I., Shimada, N., Maruyama, A.
  • Journal Title: J Control Release 149(1) Pages: 2-7
  • NAID: 120004951559
  • Peer Reviewed
• [Journal Article] Grafting of poly(ethylene glycol) to poly-lysine augments its lifetime in blood circulation and accumulation in tumors without loss of the ability to associate with siRNA (2011)
  • Author(s): Kano, A., Moriyama, K., Yamano, T., Nakamura, I., Shimada, N., Maruyama, A.
  • Journal Title: Journal of Control Release Volume: 149 Pages: 2-7
  • NAID: 120004951559
  • Peer Reviewed
• [Journal Article] Grafting of poly(ethylene glycol)to poly-lysine augments its lifetime in blood circulation and accumulation in tumors without loss of the ability to associate with siRNA (2010)
  • Author(s): Kano, A., Moriyama, K., Yamano, T., Nakamura, I., Shimada, N., Maruyama, A.
  • Journal Title: J Control Release (in press)
  • NAID: 120004951559
  • Peer Reviewed
• [Journal Article] Polymer brush-Stabilized polyplex for a siRNA carrier with long blood circulatory half-life. Adv. Mater. Res. (2008)
  • Author(s): Kano, A., Yamano, T., Choi, S.W., Maruyama, A.
  • Journal Title: Adv. Mater. Res. (Zuerich, Switz.) FIELD Full Journal Title : Advanced Materials Research (Zuerich, Switzerland)(Pt.1, Multi-Functional Materials and Structures)
  • Peer Reviewed
• [Presentation] PLL-g-PEG共重合体と光線力学療法剤の複合化と腫瘍集積性 (2010)
  • Author(s): 谷脇勇輝、森山健司、中村いずみ、嶋田直彦、狩野有宏、丸山厚
  • Organizer: 第32回日本バイオマテリアル学会大会
  • Place of Presentation: 広島
  • Year and Date: 2010-11-30
• [Presentation] Complex Formation and Tumor Accumulation of Porfimer Sodium with PLL-g-PEG Copolymer for Photodynamic Therapy (2010)
  • Author(s): Arihiro Kano, Yuuki Taniwaki, Takeshi Yamano, Izumi Nakamura, Naohiko Shimada, Atsushi Maruyama
  • Organizer: 2010 International Symposium of Materials on Regenerative Medicine
  • Place of Presentation: Zhunan, Taiwan
  • Year and Date: 2010-11-05
• [Presentation] Complex Formation and Tumor Accumulation of Porfimer Sodium With PLL-G-PEG Copolymer for Photodynamic Therapy (2010)
  • Author(s): Complex Formation and Tumor Accumulation of Porfimer Sodium With PLL-G-PEG Copolymer for Photodynamic Therapy
  • Organizer: 2010 International Symposium of Materials on Regenerative Medicine
  • Place of Presentation: Taiwan
  • Year and Date: 2010-11-05
• [Presentation] ポリ(L-リシン)-グラフト-ポリエチレングリコール共重合体(PLL-g-PEG)の光線力学療法剤ポルフィマーナトリウムとの相互作用と腫瘍デリバリー (2010)
  • Author(s): 狩野有宏、中村いずみ、森山健司、嶋田直彦、丸山厚
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-15
• [Presentation] 水溶性グラフト高分子の腫瘍集積性とドラッグデリバリー (2010)
  • Author(s): 狩野有宏
  • Organizer: 九州地区高分子若手研究会・夏の講演会
  • Place of Presentation: 北九州
  • Year and Date: 2010-07-09
• [Presentation] ドラッグデリバリー担体としてのポリエチレングリコールグラフト共重合体の評価 (2009)
  • Author(s): 森山健司, 中村いずみ, 狩野有宏, 丸山厚
  • Organizer: 第24回生体機能関連化学シンポジウム若手フォーラム
  • Place of Presentation: 福岡
  • Year and Date: 2009-09-16
• [Presentation] siRNAとの相互作用に及ぼすポリリシン側鎖にグラフトしたPEG鎖の影響 (2009)
  • Author(s): 狩野有宏, 中村いずみ, 山野剛, 嶋田直彦, 丸山厚
  • Organizer: 遺伝子・デリバリー研究会 第9回シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-11
• [Presentation] PLL-g-PEG共重合体の腫瘍集積性の解析とsiRNAデリバリーへの応用 (2008)
  • Author(s): 狩野有宏、山野剛、丸山厚
  • Organizer: 第24回日本DDS学会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-29
• [Remarks] ホームページ等
  • URL: http://www.cm.kyushu-u.ac.jp/wmaruyama/