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Patho-mechanism of transmission of low back pain

Research Project

Project/Area Number 20500439
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Rehabilitation science/Welfare engineering
Research InstitutionChiba University

Principal Investigator

OHTORI Seiji  Chiba University, 大学院・医学研究院, 助教 (40361430)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Kazuhisa  千葉大学, 大学院・医学研究院, 教授 (20179477)
TAKASO Masashi  北里大学, 医学部附属病院, 教授 (90439117)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords椎間板 / 腰痛 / 細胞内シグナル / サイトカイン / グリア / 細胞 / シグナル / 神経 / 疼痛 / 脊椎 / 伝達 / 後根神経節
Research Abstract

Study Design : Immunohistological analysis in an injured intervertebral disk (IVD) model.Objective : To elucidate and compare in rats the behavior of the sensory nervous system and inflammatory mediators in experimentally-injured IVDs.Summary of Background Data : Multiple human and animal studies have verified the presence of sensory nerve fibers in intervertebral disks or investigated the behavior of inflammatory mediators in injured IVDs, but no in vivo study to date has examined the relationship between the two.Methods : Eight-week-old female rats were used. In the disk-injured group, L5/6 disks were injured with a 24-gauge needle ; simultaneously, the neurotracer Fluoro-gold (FG) was injected into the L5/6 IVD. The L5/6 IVD, dorsal root ganglia (DRGs) from the L1 to L6 levels, and the spinal cord were resected at several time points after surgery. Nerve growth factor (NGF), tumor necrosis factor (TNF)-alpha and interrleukin (IL)-6 production in the IVDs were quantified using enzyme … More -linked immunosorbent assay (ELISA). DRGs were immunostained for calcitonin gene-related peptide (CGRP), and spinal cord sections were immunostained for ionized calcium-binding adaptor molecule-1 (iba1) and glial fibrillary acidic protein (GFAP).Results : NGF, and TNF-alpha levels (through 1 week) and IL-6 levels (through 4 days) were significantly higher in the disk-injured group than in the non-injured group (p<0.05). However, starting at 2 weeks (NGF and TNF-alpha) or 1 week (IL-6), the differences in inflammatory mediator levels between the two groups no longer were significant. In contrast, the percentage of CGRP-immunoreactive (IR) neurons among FG-labeled DRG neurons, and the numbers of iba1-IR microglia and GFAP-IR astrocytes in the spinal dorsal horn remained significantly higher in the injured group than in the non-injured group at all time points (p<0.05).Conclusion : Disk injury in rats produces persistent increases in neuropeptides in DRGs and glia in the spinal cord, but only transient increases in inflammatory mediators in IVDs. Less

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (19 results)

All 2011 2010 2009 2008

All Journal Article (17 results) (of which Peer Reviewed: 3 results) Presentation (2 results)

  • [Journal Article] Less invasive and less technically demanding decompressive procedure for lumbar spinal stenosis-appropriate for general orthopaedic surgeons?2011

    • Author(s)
      Takaso M, Nakazawa T, Imura T, Okada T, Fukushima K, Ueno M, Saito W, Shintani R, Sakagami H, Takahashi K, Yamazaki M, Ohtori S, Kotani T
    • Journal Title

      Int Orthop. 35

      Pages: 67-73

    • Related Report
      2010 Final Research Report
  • [Journal Article] Effect of applying p75NTR saporin to a punctured intervertebral disc on calcitonin gene-related peptide expression in rat dorsal root ganglion neurons.2010

    • Author(s)
      Sugiura A, Ohtori S, Yamashita M, Yamauchi K, Inoue G, Suzuki M, Norimoto M, Orita S, Eguchi Y, Kuniyoshi K, Ochiai N, Kishida S, Takaso M, Aoki Y, Ishikawa T, Arai G, Miyagi M, Kamoda H, Nakamura J, Takahashi K
    • Journal Title

      J Orthop Sci. 15

      Pages: 407-13

    • NAID

      10027404233

    • Related Report
      2010 Final Research Report
  • [Journal Article] Primary sensory neurons with dichotomizing axons projecting to the facet joint and the low back muscle in rats.2010

    • Author(s)
      Wakai K, Ohtori S, Yamashita M, Yamauchi K, Inoue G, Suzuki M, Orita S, Eguchi Y, Ochiai N, Kishida S, Takaso M, Fukui Y, Hayashi Y, Aoki Y, Kuniyoshi K, Nakamura J, Ishikawa T, Arai G, Miyagi M, Kamoda H, Takahashi K
    • Journal Title

      J Orthop Sci. 15

      Pages: 402-6

    • NAID

      10027404211

    • Related Report
      2010 Final Research Report
  • [Journal Article] Comparison of early and late surgical intervention for lumbar disc herniation : is earlier better?2010

    • Author(s)
      Akagi R, Aoki Y, Ikeda Y, Nakajima F, Ohtori S, Takahashi K, Yamagata M
    • Journal Title

      J Orthop Sci. 15

      Pages: 294-8

    • NAID

      10027403878

    • Related Report
      2010 Final Research Report
  • [Journal Article] Evaluation of behavior and neuropeptide markers of pain in a simple, sciatic nerve-pinch pain model in rats.2010

    • Author(s)
      Hirose K, Iwakura N, Orita S, Yamashita M, Inoue G, Yamauchi K, Eguchi Y, Ochiai N, Kishida S, Nakamura J, Takaso M, Ishikawa T, Arai G, Miyagi M, Kamoda H, Aoki Y, Hiwatari R, Kakizaki J, Kunishi T, Kono M, Suzuki T, Toyone T, Takahashi K, Kuniyoshi K, Ohtori S.
    • Journal Title

      Eur Spine J. 19

      Pages: 1746-52

    • Related Report
      2010 Final Research Report
  • [Journal Article] Low back pain after lumbar discectomy in patients showing endplate modic type 1 change.2010

    • Author(s)
      Ohtori S, Yamashita M, Yamauchi K, Inoue G, Koshi T, Suzuki M, Orita S, Eguchi Y, Ochiai N, Kishida S, Takaso M, Aoki Y, Ishikawa T, Arai G, Miyagi M, Kamoda H, Nakamura J, Takahashi K
    • Journal Title

      Spine. 35

    • Related Report
      2010 Final Research Report
  • [Journal Article] Two-year results for scoliosis secondary to Duchenne muscular dystrophy fused to lumbar 5 with segmental pedicle screw instrumentation.2010

    • Author(s)
      Takaso M, Nakazawa T, Imura T, Okada T, Toyama M, Ueno M, Fukushima K, Saito W, Minatani A, Miyajima G, Fukuda M, Takahira N, Takahashi K, Yamazaki M, Ohtori S, Okamoto H, Okutomi T, Okamoto M, Masaki T
    • Journal Title

      J Orthop Sci. 15

      Pages: 171-7

    • NAID

      10027403332

    • Related Report
      2010 Final Research Report
  • [Journal Article] Direct evidence for sensory innervation of the dorsal portion of the Co5/6 coccygeal intervertebral disc in rats.2010

    • Author(s)
      Orita S, Ohtori S, Taniguchi A, Yamashita M, Yamauchi K, Inoue G, Suzuki M, Eguchi Y, Ochiai N, Kishida S, Takaso M, Aoki Y, Takahashi K, Kawakami M
    • Journal Title

      Spine. 35

      Pages: 1346-52

    • NAID

      10028065427

    • Related Report
      2010 Final Research Report
  • [Journal Article] Development of new osteonecrosis in systemic lupus erythematosus patients in association with long-term corticosteroid therapy after disease recurrence.2010

    • Author(s)
      Nakamura J, Ohtori S, Sakamoto M, Chuma A, Abe I, Shimizu K
    • Journal Title

      Clin Exp Rheumatol. 28

      Pages: 13-8

    • Related Report
      2010 Final Research Report
  • [Journal Article] Sclerotomes in the Thoracic and Lumbar Spine, Pelvis, and Hindlimb Bones of Rats.2010

    • Author(s)
      Takahashi Y, Ohtori S, Takahashi K
    • Journal Title

      J Pain. 11

      Pages: 652-662

    • Related Report
      2010 Final Research Report
  • [Journal Article] Can the caudal extent of fusion in the surgical treatment of scoliosis in Duchenne muscular dystrophy be stopped at lumbar 5?2010

    • Author(s)
      Takaso M, Nakazawa T, Imura T, Ueno M, Saito W, Shintani R, Takahashi K, Yamazaki M, Ohtori S, Okamoto M, Masaki T, Okamoto H, Okutomi T, Ishii K, Ueda Y
    • Journal Title

      Eur Spine J. 19

      Pages: 787-96

    • Related Report
      2010 Final Research Report
  • [Journal Article] Existence of pyogenic spondylitis in Modic type 1 change without other signs of infection : 2-year follow-up.2010

    • Author(s)
      Ohtori S, Koshi T, Yamashita M, Yamauchi K, Inoue G, Suzuki M, Takaso M, Orita S, Eguchi Y, Ochiai N, Kishida S, Kuniyoshi K, Nakamura J, Aoki Y, Ishikawa T, Arai G, Miyagi M, Kamoda H, Takahashi K
    • Journal Title

      Eur Spine J. 19

      Pages: 1200-5

    • Related Report
      2010 Final Research Report
  • [Journal Article] Change in Modic Type 1 and 2 signals after posterolateral fusion surgery.2010

    • Author(s)
      Ohtori S, Yamashita M, Yamauchi K, Inoue G, Koshi T, Suzuki M, Orita S, Eguchi Y, Ochiai N, Kishida S, Takaso M, Kuniyoshi K, Ishikawa T, Arai G, Miyagi M, Kamoda H, Nakamura J, Aoki Y, Takahashi K
    • Journal Title

      Spine. 35

      Pages: 1231-5

    • NAID

      10028064843

    • Related Report
      2010 Final Research Report
  • [Journal Article] Surgical management of severe scoliosis with high risk pulmonary dysfunction in Duchenne muscular dystrophy : patient function, quality of life and satisfaction.2010

    • Author(s)
      Takaso M, Nakazawa T, Imura T, Okada T, Fukushima K, Ueno M, Takahira N, Takahashi K, Yamazaki M, Ohtori S, Okamoto H, Okutomi T, Okamoto M, Masaki T, Uchinuma E, Sakagami H
    • Journal Title

      Int Orthop. 34

      Pages: 695-702

    • Related Report
      2010 Final Research Report
  • [Journal Article] Effectiveness of L2 spinal nerve infiltration for selective discogenic low back pain patients.2010

    • Author(s)
      Ohtori S, et al.
    • Journal Title

      J Orthop Sci.

      Volume: 15 Pages: 731-736

    • NAID

      10027872438

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Results of surgery for discogenic low back pain : a randomized study using discography versus discoblock for diagnosis.2009

    • Author(s)
      Ohtori S, et al.
    • Journal Title

      SPINE 34

      Pages: 1435-8

    • NAID

      10027243675

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The p75 receptor is associated with inflammatory thermal hypersensitivity2008

    • Author(s)
      Watanabe T, Ito T, Inoue G, Ohtori S, Kitajo K, Doya H, Takahashi K, Yamashita T.
    • Journal Title

      J Neurosci Res. 86

      Pages: 3566-3574

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Takahashi K Disk injury produces transient increase of inflammatory cytokines in disks, whereas long-lasting increase painful neuropeptide in sensory nerve innervating disks in rats. Patho-mechanism of chronic diskogenic low back pain.2010

    • Author(s)
      Miyagi M, Ishikawa T, Orita S, Eguchi Y, Kamoda H, Arai G, Suzuki M, Ohtori S
    • Organizer
      Oral Presentation WFSR (World Forum for Spine Research)
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2010-07-10
    • Related Report
      2010 Final Research Report
  • [Presentation] Takahashi K Disk injury produces transient increase of inflammatory cytokines in disks, whereas long-lasting increase painful neuropeptide in sensory nerve innervating disks in rats.2010

    • Author(s)
      Miyagi M, Ishikawa T, Orita S, Eguchi Y, Kamoda H, Arai G, Suzuki M, Inoue G, Ohtori S
    • Organizer
      Special Poster ISSLS (International Society for the Study of the Lumbar Spine)
    • Place of Presentation
      Auckland, NZ
    • Year and Date
      2010-04-10
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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