Mechanism of muscle atrophy in microgravity environment

• Project/Area Number: 20500447
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Hiroshima University
• Principal Investigator: KAWAHARA Yumi Hiroshima University, 病院, 研究員 (20457279)
• Co-Investigator(Kenkyū-buntansha): YUGE Rui 広島大学, 大学院・保健学研究科, 教授 (20263676)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 微小重力環境 / 筋芽細胞 / 筋分化抑制 / 重力 / 筋萎縮 / 筋委縮

Research Abstract

L6 rat myoblast cells were induced differentiation in 1G environment (group 1G) or 10^<-3>G environment (group CL). After 5 days induced differentiation, cells were divided into two more groups and cultured for another 5 days. Subject was continued to culture in same environment (group CL/CL, group 1G/1G), the other subject was exchanged culture environment of the cells (group 1G/CL, group CL/1G). Microgravity inhibited myoblast differentiation by morphological observation and molecular biological analysis. The cells in group 1G/CL suppressed differentiation when cells in 10^<-3>G environment, while the cells in group CL/1G began differentiation when cells in 1G environment.

Research Products

• [Journal Article] Simulated microgravity maintains the undifferentiated state and enhances the neural repair potential of bone marrow stromal cells. (2011)
  • Author(s): Yuge L., Sasaki A,, Kawahara Y., Wu SL., Matsumoto M., Manabe T., Kajiume T., Takeda M., Magaki T., Takahashi T., Kurisu K., Matsumoto M.
  • Journal Title: Stem Cells Dev in press
  • Peer Reviewed
• [Journal Article] Simulated microgravity maintains the undifferentiated state and enhances the neural repair potential of bone marrow stromal cells. (2011)
  • Author(s): Yuge L., Sasaki A, Kawahara Y., Wu SL., Matsumoto M., Manabe T., Kajiume T., Takeda M., Magaki T., Takahashi T., Kurisu K., Matsumoto M.
  • Journal Title: Stem Cells Dev Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] LIF-free embryonic stem cell culture in simulated microgravity. (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M., Kajiume T., Matsumoto M., Yuge L.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] LIF-free embryonic stem cell culture in simulated microgravity (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M., Kajiume T., Matsumoto M., Yuge L.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Preventive effect of isometric contraction exercise on disuse muscle atrophy using tail suspension mice. (2008)
  • Author(s): Kawahara Y., Nikawa T., Hirasaka K., Miyashita T., Kataoka K., Yuge L.
  • Journal Title: Journal of Physical Therapy Science 20 Pages: 39-44
  • NAID: 10024158950
  • Peer Reviewed
• [Journal Article] Preventive effect of isometric contraction exercise on disuse muscle atrophy using tail suspension mice. (2008)
  • Author(s): Kawahara Y, (他5名)
  • Journal Title: Journal of Physical Therapy Science 20 Pages: 39-44
  • NAID: 10024158950
  • Peer Reviewed
• [Presentation] 重力による細胞分化の促進と抑制 (2011)
  • Author(s): 森川久美, 松本昌也, 深澤賢宏, 猪村剛史, 孫亜楠, 河原裕美, 弓削類
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] A specific-gravity medium : novel approach for mouse hematopoietic stem cell culture. (2010)
  • Author(s): Kawahara Y., Kajiume T., Matsumoto M. Imura T., Matsumoto M., Yuge L.
  • Organizer: 8^<th> International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2010-06-18
• [Presentation] Aggregates formation of mouse ES cells using simulated microgravity and neural differentiation. (2010)
  • Author(s): 猪村剛史, 松本昌也, 河原裕美, 弓削類
  • Organizer: 8^<th> International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2010-06-17
• [Presentation] 模擬微小重力環境下での細胞塊形成と神経分化能 (2010)
  • Author(s): 猪村剛史, 松本昌也, 河原裕美, 弓削類
  • Organizer: 第45回日本理学療法学学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2010-05-29
• [Presentation] 微小重力環境と幹細胞の未分化維持-マウスES細胞での検討- (2010)
  • Author(s): 河原裕美, 松本昌也, 猪村剛史, 松本昌泰, 弓削類
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-18
• [Presentation] 模擬微小重力環境下での細胞塊形成と神経分化能 (2010)
  • Author(s): 猪村剛史, 松本昌也, 河原裕美, 弓削類
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-18
• [Presentation] Simulated microgravity makes mouse embryonic stem cells aggregate to maintain pluripotency in LIF-free culture. (2010)
  • Author(s): Kawahara Y., Kajiume T., Matsumoto M., Fukazawa T., Imura T., Morikawa K., Matsumoto M., Yuge L
  • Organizer: BIT's 3^<rd> Annual World Congress of Regenerative Medicine & Stem Cells (RMSC) Everbright Convention & Exhibition Center
  • Place of Presentation: Shanghai, China
• [Presentation] Gravity affects myoblast differentiation and undifferentiation -Myotub developmen study-. (2010)
  • Author(s): Morikawa K., Matsumoto M., Fukazawa T., Imura T., Sun YN., Khalesi E., Kawahara Y., Yuge L
  • Organizer: BIT's 3^<rd> Annual World Congress of Regenerative Medicine & Stem Cells (RMSC) 2010 Everbright Convention & Exhibition Center
  • Place of Presentation: Shanghai, China
• [Presentation] Gravity affects myoblast differentiation and undifferentiation-Myotub developmen study-. (2010)
  • Author(s): Morikawa K., Matsumoto M., Fukazawa T., Imura T., Sun YN., Khalesi E., Kawahara Y, Yuge L.
  • Organizer: BIT's 3^<rd> Annual World Congress of Regenerative Medicine & Stem Cells (RMSC) 2010
  • Place of Presentation: Shanghai, China
• [Presentation] Simulated microgravity makes mouse embryonic stem cells aggregate to maintain pluripotency in LIF-free culture. (2010)
  • Author(s): Kawahara Y., Kajiume T., Matsumoto M., Fukazawa T., Imura T., Morikawa K., Matsumoto M., Yuge L.
  • Organizer: BIT's 3^<rd> Annual World Congress of Regenerative Medicine & Stem Cells (RMSC) 2010
  • Place of Presentation: Shanghai, China
• [Presentation] Simulated microgravity : a novel approach to embryonic stem cell culture. (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M, Imura T., Kajiume T., Takeda M., Matsumoto M., Yuge L.
  • Organizer: American Society for Gravitational and Space Biology (ASGSB 25th Annual Meeting NASA)
  • Place of Presentation: Raleigh, NC, USA
  • Year and Date: 2009-11-07
• [Presentation] Simulated microgravity : a novel approach to embryonic stem cell culture (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M, Imura T., Kajiume T., Takeda M., Matsumoto M., Yuge L.
  • Organizer: American Society for Gravitational and Space Biology (ASGSB 25th Annual Meeting NASA)
  • Place of Presentation: Raleigh, NC, USA
  • Year and Date: 2009-11-07
• [Presentation] Transplantation of bone marrow stromal cells cultured under simulated microgravity into a spinal cord injury rat. (2009)
  • Author(s): Takeda M., Magaki T., Sasaki A., Manabe T., Matsumoto M., Kawahara Y., Yuge L., Kurisu K.
  • Organizer: American Society for Gravitational and Space Biology (ASGSB 25^<th> Annual Meeting NASA)
  • Place of Presentation: Raleigh, NC, USA
  • Year and Date: 2009-11-06
• [Presentation] Transplantation of bone marrow stromal cells cultured under simulated microgravity into a spinal cord injury rat (2009)
  • Author(s): Takeda M., Magaki T., Sasaki A., Manabe T., Matsumoto M., Kawahara Y., Yuge L., Kurisu K.
  • Organizer: American Society for Gravitational and Space Biology (ASGSB 25th Annual Meeting NASA)
  • Place of Presentation: Raleigh, NC, USA
  • Year and Date: 2009-11-06
• [Presentation] Simulated microgravity enables LIF-free culture in mouse embryonic stem cells. (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M., Ogawa K., Kajiume T., Takeda M., Magaki T., Yamashita H., Takahashi T., Aoki S., Sueda Y., Matsumoto M., Yuge L.
  • Organizer: 7^<th> International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2009-07-09
• [Presentation] Simulated microgravity enables LIF-free culture in mouse embryonic stem cells (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M., Ogawa K., Kajiume T., Takeda M., Magaki T., Yamashita H., Takahashi T., Aoki S., Sueda Y., Matsumoto M., Yuge L.
  • Organizer: 7th International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2009-07-09
• [Presentation] ラットにおける脊髄損傷後の微小重力培養骨髄由来細胞移植 (2009)
  • Author(s): 真鍋朋誉, 武田正明, 松本昌也, 小川和幸, 河原裕美, 弓削類
  • Organizer: 第44回日本理学療法学学術大会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-30
• [Presentation] Gravity affects to myoblast differentiation. (2008)
  • Author(s): Kawahara Y., Manabe T., Ogawa K., Matsumoto M., Yamashita H., Takahashi T., Aoki S., Sueda Y., Matsumoto M., Yuge L.
  • Organizer: The ESA ISGP ASGSB ELGRA Meeting "Life in Space for Life on Earth"e for Life on Earth" (29^<th> Annual ISGP Meeting, 10^<th> ESA Life Sciences Symposium, 24^<th> Annual ASGSB Meeting, ELGRA Symposium)
  • Place of Presentation: Anger, France
  • Year and Date: 2008-06-25
• [Presentation] Gravity affects to myoblast differentiation. (2008)
  • Author(s): Kawahara Y.
  • Organizer: The ESA ISQP ASGSB ELGRA Meeting "Life in Space for Life on Earth"e for Life on Earth"
  • Place of Presentation: Anger, France
  • Year and Date: 2008-06-25
• [Presentation] Microgravity environment is a novel control approach for myoblast differentiation. (2008)
  • Author(s): Kawahara Y., Manabe T., Ogawa K., Matsumoto M., Yamashita H., Takahashi T., Aoki S., Sueda Y., Matsumoto M., Yuge L.
  • Organizer: BIT's 1^<st> Annual World Congress of Regenerative Medicine & Stem Cell 2008, Sanshui Garden Hotel (FSGH)
  • Place of Presentation: Foshan, China
• [Presentation] Microgravity environment is a novel control approach for myoblast differentiation. (2008)
  • Author(s): Kawahara Y.
  • Organizer: BIT's 1st Annual World Congress of Regenerative Medicine & Stem Cell 2008
  • Place of Presentation: Foshan, China