Effects of exercise-induced interleukin-6 secretion on protein synthesis in skeletal muscle

• Project/Area Number: 20500577
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: Osaka University
• Principal Investigator: NAKAI Naoya Osaka University, 大学院・医学系研究科, 准教授 (90324508)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 機械的ストレッチ / タンパク質合成 / チロシンキナーゼ / 伸展刺激 / チロシンリン酸化 / p70 S6 kinase / phospholipase C / eukaryotic elongation factor 2 / インターロイキン-6 / 骨格筋

Research Abstract

Murine myoblast cell line was mechanically stretched as a model for exercise-induced muscle contraction. Mechanical stretch increased the gene expression of interleukin-6 and activated the protein translation initiation. Stretch-induced activation of the protein translation initiation was completely blocked by tyrosin kinase inhibitor, genistein. Among the cellular protein which is activated by tyrosine kinase, phospholipase C was important for the signal transduction of mechanical stretch-induced activation of protein translation initiation.

Research Products

• [Journal Article] Effects of creatine and its analog, β-guanidinopropionic acid, on the differentiation of and nucleoli in myoblasts. (2011)
  • Author(s): Ohira, Y., Matsuoka, Y., Kawano, F., Ogura, A., Higo, Y., Ohira, T., Terada, M., Oke, Y., Nakai, N.
  • Journal Title: Biosci.Biotechnol.Biochem 印刷中
  • NAID: 10029327954
  • Peer Reviewed
• [Journal Article] Mechanical stretch activates signaling events for protein translation initiation and elongation in C2C12 myoblasts. (2010)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Ohira, T., Fujita, R., Ohira, Y.
  • Journal Title: Mol.Cells. 30 Pages: 513-518
  • Peer Reviewed
• [Journal Article] Role(s) of gravitational loading during developing period on the growth of rat soleus muscle fibers. (2010)
  • Author(s): Kawano, F., Goto, K., Wang, XD., Terada,M., Ohira, T., Nakai, N., Yoshioka, T., Ohira, Y.
  • Journal Title: J.Appl.Physiol. 108(3) Pages: 676-685
  • Peer Reviewed
• [Journal Article] Myonucleus-related properties in soleus muscle fibers of mdx mice. (2010)
  • Author(s): Terada, M., Lan, Y.B., Kawano, F., Ohira, T., Higo, Y., Nakai, N., Imaizumi, K., Ogura, A., Nishimoto, N., Adachi, Y., Ohira, Y.
  • Journal Title: Cells Tissues Organs. 191 Pages: 248-259
  • Peer Reviewed
• [Journal Article] Mechanical stretch activates signaling events for protein translation initiation and elongation in C2C12 myoblasts. (2010)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Ohira, T., Fujita, R, Ohira, Y.
  • Journal Title: Molecules and Cells Volume: 30 Pages: 513-518
  • Peer Reviewed
• [Journal Article] Role(s) of gravitational loading during developing period on the growth of rat soleus muscle fibers. (2010)
  • Author(s): Kawano, R, Goto, K., Wang, XD., Terada, M., Ohira, T., Nakai, N., Yoshioka, T., Ohira, Y.
  • Journal Title: Journal of Appllied Physiology Volume: 108(3) Pages: 676-685
  • Peer Reviewed
• [Journal Article] Myonucleus-related properties in soleus muscle fibers of mdx mice (2009)
  • Author(s): Terada, M., Lan, Y.B., Kawano, F., Ohira, T., Higo, Y., Nakai, N., Imaizumi, K, Ogura, A., Nishimoto, N., Adachi, Y., Ohira, Y.
  • Journal Title: Cells Tissues Organs 191 Pages: 248-259
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Naoya Nakai, Yoshinobu Ohira
  • Journal Title: Muscle Cell Physiology(Osaka University Press) Pages: 3-14
• [Journal Article] Effects of peroxisome proliferator-activated receptor α (PPARα) agonists on leucine-induced phosphorylation of translational targets in C2C12 cells. (2008)
  • Author(s): Nakai, N., Kawano, F., Terada, M., Oke, Y., Ohira, T., Ohira, Y.
  • Journal Title: Biochim.Biophys.Acta. 1780(10) Pages: 1101-1105
  • Peer Reviewed
• [Journal Article] Essential role of satellite cells in the growth of rat soleus muscle fibers. (2008)
  • Author(s): Kawano, F., Takeno, Y., Nakai, N., Higo, Y., Terada, M., Ohira, T., Nonaka, I., Ohira, Y.
  • Journal Title: Am.J.Physiol.Cell Physiol. 295(2)
  • Peer Reviewed
• [Journal Article] Essential role of satellite cells in the growth of rat soleus muscle fibers. (2008)
  • Author(s): Kawano, F., Takeno, Y., Nakai, N., Higo, Y., Terada, M., Ohira, T., Nonaka, I., Ohira, Y.
  • Journal Title: Am. J. Physiol. Cell Physiol. 295
  • Peer Reviewed
• [Journal Article] Effects of peroxisome proliferator-activated receptor α (PPARα)agonists on leucine-induced phosphorylation of translational targets in C2Cl2 cells. (2008)
  • Author(s): Nakai, N., Kawano, F., Terada, M., Oke, Y., Ohira, T., Ohira, Y.
  • Journal Title: Biochim. Biophys. Acta. 1780 Pages: 1101-1105
  • Peer Reviewed
• [Presentation] Tyrosine phosphorylation regulates mechanical stretch-induced activation of protein translation initiation in C2C12 myoblast (2010)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Ohira, T., Fujita, R., Ohira, Y.
  • Organizer: Experimental Biology2010
  • Place of Presentation: Anaheim, California(USA)
  • Year and Date: 2010-04-27
• [Presentation] Tyrosine phosphorylation regulates mechanical stretch-induced activation of protein translation initiation in C2C12 myoblast. (2010)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Ohira, T., Fujita, R., Ohira, Y.
  • Organizer: Experimental Biology 2010.
  • Place of Presentation: Anaheim, California, USA
• [Presentation] Mechanical stretch induces activation of eukaryotic elongation factor 2 in C2C12 myoblasts (2009)
  • Author(s): N. Nakai, F. Kawano, Y. Oke, S. Nomura, M. Terada, T. Ohira, and Y, Ohira
  • Organizer: Experimental Biology 09
  • Place of Presentation: New Orleans, Louisiana, USA
  • Year and Date: 2009-04-19
• [Presentation] Mechanical stretch induces activation of eukaryotic elongation factor 2 in C2C12 myoblast. (2009)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Terada, M., Ohira, T., Ohira, Y.
  • Organizer: Experimental Biology 2009.
  • Place of Presentation: New Orleans, Louisiana, USA
• [Presentation] Protein translation initiation is activated by mechanical stretch in C2C12 myoblast. (2009)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Terada, M., Ohira, T., Ohira, Y.
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
• [Presentation] Mechanical stretch induces activation of eukaryotic elongation factor 2 in C2Cl2 myoblast (2009)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Terada, M., Ohira, T., Ohira, Y.
  • Organizer: Experimental Biology 2009
  • Place of Presentation: New Orleans, Louisiana, USA
• [Presentation] Protein translation initiation is activated by mechanical stretch in C2Cl2 myoblast (2009)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Terada, M., Ohira, T., Ohira, Y.
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
• [Book] Muscle Cell Physiology (2009)
  • Author(s): Naoya Nakai, Yoshinobu Ohira
  • Total Pages: 136
  • Publisher: Osaka University Press