Effects of lengthening contractions on signaling molecules expressions in muscular protein synthesis and degradation

• Project/Area Number: 20500584
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: Nippon Sport Science University
• Principal Investigator: NAKAZATO Koichi Nippon Sport Science University, 大学院・体育科学研究科, 教授 (00307993)
• Co-Investigator(Kenkyū-buntansha): OCHI Eisuke 明治学院大学, 教養教育センター, 講師 (90468778)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: スポーツ生理学 / 伸張性収縮 / 角速度 / 神経損傷 / p75NTR / シュワン細胞 / タンパク質分解 / 筋肥大 / 筋損傷

Research Abstract

We performed eccentric contractions (ECs) with fast (180 deg/sec : 180ECs) and slow (30 deg/sec : SLOWECs) velocities on rat gastrocnemius and examined effects on protein synthesis and degradation. Obtained results are shown below. (1) 30ECs induced protein synthesis. On the other hand, 180ECs induced protein degradation. (2) Especially in 180ECs, we found evidences for both AMPK activation and sciatic nerve injury. Eccentric contractions induce either protein synthesis or degradation depending on angular velocity.

Research Products

• [Journal Article] Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle. (2011)
  • Author(s): Ochi E, Nakazato K, Ishii N
  • Journal Title: J Strength Cond Res. (in press)
  • Peer Reviewed
• [Journal Article] Time course change of IGF1/Akt/mTOR/p70S6K pathway activation in rat gastrocnemius muscle during repeated bouts of eccentric exercise (2010)
  • Author(s): Ochi E, Ishii N, Nakazato K
  • Journal Title: J Sports Sci & Med. 9 Pages: 170-175
  • Peer Reviewed
• [Journal Article] Elevation of myostatin and FOXOs in prolonged muscular impairment induced by eccentric contractions in rat medial gastrocnemius muscle (2010)
  • Author(s): Ochi E, Hirose T, Hiranuma K, Min SK, Ishii N, Nakazato K
  • Journal Title: J Appl Physiol 108 Pages: 306-313
  • Peer Reviewed
• [Journal Article] Time course change of IGF1/Akt/mTOR/p70S6K pathway activation in rat gastrocnemius muscle during repeated bouts of eccentric exercise (2010)
  • Author(s): Ochi, et al.
  • Journal Title: J Sports Sci & Med Volume: 9 Pages: 170-175
  • Peer Reviewed
• [Journal Article] Elevation of atrophy-related protein in prolonged muscular impairment induced by eccentric contractions in rat medial gastrocnemius muscle (2010)
  • Author(s): Ochi, et al.
  • Journal Title: Journal of Applied Physiology 108 Pages: 306-313
  • Peer Reviewed
• [Journal Article] Dietary apple polyphenols have preventive effects against lengthening contraction-induced muscle injuries (2010)
  • Author(s): Nakazato, et al.
  • Journal Title: Mol Nutr & Food Res 54 Pages: 363-372
  • Peer Reviewed
• [Journal Article] Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle
  • Author(s): Ochi, et al.
  • Journal Title: J Strength Cond Res Volume: (In press)
  • Peer Reviewed
• [Presentation] 伸張性収縮時の角速度の違いが骨格筋内tenomodulin発現に与える影響 (2010)
  • Author(s): 越智英輔,廣瀬立朗,平沼憲治,石井直方,中里浩一
  • Organizer: 第65回日本体力医学会大会
  • Place of Presentation: 千葉
• [Presentation] Challenge to elucidate the molecular events in sports injuries (2009)
  • Author(s): Nakazato K
  • Organizer: International Sports Science Congress for the memorial of 30th anniversary
  • Place of Presentation: Hanyang University, Korea
  • Year and Date: 2009-08-18
• [Presentation] 伸張性収縮時の関節角速度の差がその後の発揮トルクに与える影響 (2008)
  • Author(s): 越智英輔,廣瀬立朗,関石基,中里浩一
  • Organizer: 第63回日本体力医学会大会
  • Place of Presentation: 大分
• [Presentation] 伸張性収縮時の関節角速度の差がその後の発揮トルクに与える影響 (2008)
  • Author(s): 越智ら
  • Organizer: 体力医学会
  • Place of Presentation: 大分県別府市
• [Book] 整形・災害外科(分子レベルからみた整形外科疾患(シリーズVIII),肉離れ損傷発生および進行の分子メカニズムに関する仮説 53(3)) (2010)
  • Author(s): 中里浩一,平沼憲治,越智英輔
• [Remarks] ホームページ等
  • URL: http://www.nittai.ac.jp/department/exphys/
• [Remarks]
  • URL: http://www.nittai.ac.jp/department/exphys/