Research Project
Grant-in-Aid for Scientific Research (C)
The increase in reactive oxygen species (ROS) levels that occurs during intense exercise has been proposed to be one of the major causes of muscle fatigue. To investigate the pathological significance of oxidative stress in skeletal muscle, we generated skeletal muscle-specific manganese superoxide dismutase (Mn-SOD)-deficient (muscle-Sod2^<-/->) mice. The mutant mice showed severe disturbances in exercise activity, but no atrophic changes in their skeletal muscles. In histological and histochemical analyses, the mutant mice showed centralized nuclei in their muscle fibers and selective loss of enzymatic activity in mitochondrial respiratory chain complexes. In addition, the mutant mice displayed increased oxidative damage and reduced ATP content in their muscle tissue. Furthermore, a single administration of the antioxidant EUK-8 significantly improved exercise activity and increased the cellular ATP level in skeletal muscle. These results imply that the superoxide anions generated in mitochondria play a pivotal role in the progression of exercise intolerance.
All 2011 2010 2009 2008 Other
All Journal Article (22 results) (of which Peer Reviewed: 9 results) Presentation (22 results) Book (5 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)
J Mol Cell Cardiol 50
Pages: 670-677
120004951631
Aging Cell 10
Pages: 493-505
J Neurosci Res 89
Pages: 601-610
J Amino Acid 2011
Pages: 654207-654207
Aging Cell
Volume: 印刷中
J.Neuro.Sci.
Volume: 89 Pages: 601-610
Geriatr Gerontol Int 10
Pages: 70-79
40005949431
Free Rad Biol Med 48
Pages: 1252-1262
Free Rad.Biol.Med.
Volume: 48 Pages: 1252-1262
Free Rad.Biol.Med. 48
新老年学「第3版」(東京大学出版社)
Pages: 213-236
Am J Physiol 297
Pages: 1520-1532
Cir J 73
Pages: 2125-2134
10025937556
Biochem Biophys Res Commun 382
Pages: 457-61
Am.J.Physiol.Cell Physiol. 297
Cir.J. 73
Biochem. Biophys. Res. Commun. 382
Pages: 457-461
Mech Ageing Dev 129
Pages: 291-298
Am J Pathol 172
Pages: 1325-1331
Am. J. Pathol. 172
Mech. Ageing Dev. 129
老化・老年病のための動物実験ガイドブック(アドスリー)
Pages: 115-122
http://www.tmig.or.jp/J_TMIG/j_research/DA12.html
http://www.tmig.or.jp/J_TMIG/j_research/A11.html