Establishment of system to elucidate mechanism for beneficial effect of collagen peptide by ingestion.

• Project/Area Number: 20500712
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyoto Prefectural University
• Principal Investigator: SATO Kenji Kyoto Prefectural University, 大学院・生命環境科学研究科, 教授 (00202094)
• Co-Investigator(Kenkyū-buntansha): SHIGEMURA Yasutaka 京都府立大学 (20373178)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: コラーゲン / Pro-Hyp / 線維芽細胞 / ペプチド / 皮膚 / トランスポーター / 細胞間マトリッス / PEPT1 / PTH1 / コラーゲンペプチド / Hyp-Gly / 傷修復 / 遊走

Research Abstract

A novel food-derived collagen peptide ; Hyp-Gly was identified in the human peripheral blood. Hyp-Gly stimulated growth of primary cultured fibroblast on collagen gel. The present study also demonstrated that fibroblast migrated from mouse skin expressed peptide transporter 1 and 2 (PEPT1, 2). However, expression of PEPT1, 2 dramatically decreased after several passages. These facts suggest that absorption of the collagen peptide through PEPT1, 2 might play significant role in cell proliferation by collagen peptide.

Research Products

• [Journal Article] Identification of a novel food-derived collagen peptide, hydroxyprolyl-glycine, in human peripheral blood by pre-column derivatisation with phenyl isothiocyanate. (2011)
  • Author(s): Shigemura, Y., Akaba, S., Kawashima, E., Park E.-Y. Nakamura, Y., Sato, K.
  • Journal Title: Food Chemistry In press
  • Peer Reviewed
• [Journal Article] Identification of a Novel Food-Derived Collagen Peptide, Hydroxyprolyl-Glycine, in Human Peripberal Blood by Pre-column Derivatization with Phenyl Isothiocyanate (2011)
  • Author(s): Shigemura, Y.Akaba, S., Kawashima, E., Park, E.Y., Nakamura, Y., Sato, K.
  • Journal Title: Food Chemistry Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Hydroxyproline-containing dipeptides and tripeptides quantified at high concentration in human blood after oral administration of gelatin hydrolysate. (2010)
  • Author(s): Ichikawa, S., Morifuji, M., Ohara, H., Matsumoto, H., Takeuchi, Y., Sato, K.
  • Journal Title: Int.J.Food Sci.Nutri. 61 Pages: 52-60
  • Peer Reviewed
• [Journal Article] コラーゲンペプチド経口摂取による生理機能とそのメカニズム (2010)
  • Author(s): 重村泰毅, 佐藤健司
  • Journal Title: 食品加工技術 30 Pages: 1-7
• [Journal Article] アンチエイジングにおける機能性ペプチドの応用 (2010)
  • Author(s): 佐藤健司
  • Journal Title: Functional Food 4(1) Pages: 67-71
• [Journal Article] 高付加価値食品開発のための経口摂取により活性を持つペプチドの同定 (2010)
  • Author(s): 佐藤健司
  • Journal Title: New Food Industry 52(9) Pages: 12-20
  • NAID: 40017275184
• [Journal Article] Hydroxyproline-Containing Dipeptides and Tripeptides Quantified at High Concentration in Human Blood after Oral Administration of Gelatin Hydrolysate (2010)
  • Author(s): Ichikawa, S., Morifuji, M., Ohara, H., Matsumoto, H., Takeuchi, Y., Sato, K.
  • Journal Title: Int.J.Food Sci.Nutri. Volume: 61 Pages: 52-60
  • Peer Reviewed
• [Journal Article] コラーゲンペプチド経口摂取による生理機能とそのメカニズム (2010)
  • Author(s): 重村泰毅, 佐藤健司
  • Journal Title: 食品加工技術 Volume: 30 Pages: 1-7
• [Journal Article] コラーゲンペプチド経口摂取による生理機能とそのメカニズム (2010)
  • Author(s): 重村泰毅
  • Journal Title: 食品加工技術 30 Pages: 1-7
• [Journal Article] Effect of prolyl-hydroxyproline (Pro-Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin. (2009)
  • Author(s): Shigemura, Y., Iwai, K., Morimatsu, F., Iwamoto, T., Mori, T., Oda, C. Taira, T., Park, E.Y., Nakamura, Y., Sato, K.
  • Journal Title: J.Agric.Food Chem. 57(2) Pages: 444-449
  • Peer Reviewed
• [Journal Article] コラーゲンペプチドの生理機能 (2009)
  • Author(s): 佐藤健司
  • Journal Title: グルコサミン研究 5 Pages: 79-88
• [Journal Article] Hydroxyproline-cntaining dipeptides and tripeptides quantified at high concentration in human blood after oral administration of gelatin hydrolysate (2009)
  • Author(s): Ichikawa, S.
  • Journal Title: Int.J.Food Sci.Nutri. 61 Pages: 52-60
  • Peer Reviewed
• [Journal Article] Characterization and comparison of collagens extracted from the digestive tract and skin of a Japanese amber jack (Seriola quinoqeradiata) (2009)
  • Author(s): Nishimoto, M.
  • Journal Title: J.Food Biochem. 33 Pages: 777-789
  • Peer Reviewed
• [Journal Article] ヒトの末梢血中の食事由来コラーゲンペプチドの構造と機能 (2009)
  • Author(s): 佐藤健司
  • Journal Title: Food Style 13 Pages: 51-54
• [Journal Article] Effect of prolyl-hydroxyproline (Pro-Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin. (2009)
  • Author(s): Shigemura, Y.
  • Journal Title: J.Agric.Food Chem., 57 Pages: 444-449
  • Peer Reviewed
• [Journal Article] Effect of prolyl-hydroxyproline (Pro-Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin. (2009)
  • Author(s): Shigemura, Y, 他
  • Journal Title: J. Agric. Food Chem. 57 Pages: 444-449
  • Peer Reviewed
• [Journal Article] ヒト末梢血中の食事由来コラーゲンペプチドの構造と機能 (2009)
  • Author(s): 佐藤健司
  • Journal Title: Food Style 13 Pages: 51-54
• [Journal Article] Identification of food-derived bioactive peptides in blood and other biological samples. (2008)
  • Author(s): Sato, K. Iwai, K., Aito-Inoue, M.
  • Journal Title: J.AOAC Int. 91(4) Pages: 995-1001
  • Peer Reviewed
• [Journal Article] コラーゲンペプチドの経口摂取による生理機能 (2008)
  • Author(s): 佐藤健司, 他
  • Journal Title: グルコサミン研究 4 Pages: 57-62
• [Presentation] Impact of extracellular matrix protein hydrolysates on human health. (2011)
  • Author(s): Sato, K.
  • Organizer: 102nd AOCS Annual Meeting & Expo.
  • Place of Presentation: Cincinnati, USA
  • Year and Date: 2011-05-03
• [Presentation] Impact of Extracellular Matrix Protein Hydrolysates on Human Health (2011)
  • Author(s): Sato, K
  • Organizer: American Oil Chemist's Society, Annual Meeting
  • Place of Presentation: シンシナティ(米国)
  • Year and Date: 2011-05-03
• [Presentation] Identification of Food-derived peptides in human peripheral blood-Good candidate for active peptide with health-promotion activity-. (2010)
  • Author(s): Sato, K.
  • Organizer: PepCon 2010
  • Place of Presentation: Beijing, Chaina
  • Year and Date: 2010-03-22
• [Presentation] Identification of Food-Derived Peptides in Human Peripheral Blood-Good Candidate for Active Peptide with Health-Promotion Activity- (2010)
  • Author(s): Sato, K.
  • Organizer: PepCon 2010
  • Place of Presentation: Beijing Chaina
• [Presentation] Occurrence of Food-Derived Peptides with Biological Activity in Human Blood. (2009)
  • Author(s): Sato, K.
  • Organizer: Annual Meeting of International Society for Neutraceuticals and Functional Foods
  • Place of Presentation: San Francisco
  • Year and Date: 2009-11-03
• [Presentation] Occurrence of Food-Derived Peptides with Biological Activity in Human Blood. (2009)
  • Author(s): Sato, K.
  • Organizer: 3rd International Congress on Food and Nutrition
  • Place of Presentation: Antalya Turkey
  • Year and Date: 2009-04-24
• [Presentation] Occurrence of food-derived peptides with biological. activity in human blood (2009)
  • Author(s): Sato, K.
  • Organizer: International Congress on Food and Nutrition
  • Place of Presentation: Antalya Turkey
  • Year and Date: 2009-04-24
• [Presentation] Effect of Prolyl-Hydroxyproline (Pro-Hyp) on proliferation of skin fibroblasts : A possible mechanism for improvement of skin condition by collagen peptide ingestion (2009)
  • Author(s): Shigemura Y ら
  • Organizer: American Chemical Society
  • Place of Presentation: Salt Lake City, USA
  • Year and Date: 2009-03-24
• [Presentation] Biological Activity of Food-Derived Peptides in Human Blood (2009)
  • Author(s): Sato, K.
  • Organizer: International Society for Neutraceuticals and Functional Foods
  • Place of Presentation: San Francisco, CA
• [Presentation] Occurrence of Food-Derived Peptides with Biological Activity in Human Blood (2009)
  • Author(s): Sato, K.
  • Organizer: 3rd International Congress on Food and Nutrition
  • Place of Presentation: Antalya, Turkey
• [Presentation] Growth enhancement of skin fibroblast by food-derived collagen peptide in human blood (2008)
  • Author(s): Shigemura Y. ら
  • Organizer: International Society for Nutraceuticals and Functional Foods
  • Place of Presentation: Taichung, Taiwan
  • Year and Date: 2008-11-16
• [Presentation] 食事由来主要コラーゲンペプチド(Pro-Hyp, Hyp-Gly)がマウス皮膚線維芽細胞増殖におよぼす影響 (2008)
  • Author(s): 重村泰毅ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 亀岡市
  • Year and Date: 2008-09-13
• [Presentation] コラーゲンペプチドを摂取したヒト末梢血中のコラーゲンペプチド構造の個人差 (2008)
  • Author(s): 佐藤健司ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 亀岡市
  • Year and Date: 2008-09-13
• [Presentation] Subject to subject change in composition of food-derived collagen peptides in human peripheral blood after ingestion of enzymatic digest of gelatin. (2008)
  • Author(s): Sato, K.
  • Organizer: Institute of Food Technologist Annual Meeting
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2008-07-29
• [Presentation] Subject to subject change in composition of food-derived collagen peptides in human peripheral blood after ingestion of enzymatic digest of gelatin (2008)
  • Author(s): Sato, K. ら
  • Organizer: Institute of Food Technologist
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2008-06-30
• [Book] Marine Cosmeceuticals : Latest Trends and Prospects (2011)
  • Author(s): Sato, K., Shigemura, Y.
  • Publisher: CRC Press(印刷中)
• [Book] Marine Cosmeceuticals : Latest Trends and Prospects, 共著
  • Author(s): ato, K., Shigemura, Y.
  • Publisher: CRC Press 印刷中
• [Remarks] ホームページ等
  • URL: http://www.senryaku-kyoto.jp/contents/search/index.php?mode=show&seq=1165
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  • URL: http://cocktail.kpu.ac.jp/shomuka/gakujyutsu/database_index.html
• [Patent(Industrial Property Rights)] 創傷治療用医薬組成物 (2009)
  • Inventor(s): 佐藤健司ら
  • Industrial Property Rights Holder: チッソ株式会社
  • Industrial Property Number: 2009-042671
  • Filing Date: 2009-02-25