Study for the development of a novel treatment to prevent atherosclerosis with guava tea

• Project/Area Number: 20500713
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Okayama Prefectural University
• Principal Investigator: TAKAHASHI Yoshitaka Okayama Prefectural University, 保健福祉学部, 教授 (10236333)
• Co-Investigator(Kenkyū-buntansha): KAWAKAMI Yuki 岡山県立大学, 保健福祉学部, 助教 (30453202)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 12-リポキシゲナーゼ / アラキドン酸 / 酸化LDL / 動脈硬化 / グアバポリフェノール / グアバ葉 / 12/15-リポキシゲナーゼ / 12 / 15-リポキシゲナーゼ / グアバ茶 / ケルセチン

Research Abstract

Oxidative modification of low density lipoprotein (LDL) is one of the critical steps for the development of atherosclerosis and leukocyte-type 12-lipoxygenase has been suggested to play an essential role in this process. In this study, we demonstrated that guava leaf extracts inhibited not only the leukocyte-type 12-lipoxygenase activity but also LDL oxidation mediated by the enzyme-overexpressing J774A.1 cells. Oral administration of guava leaf extracts to apo E-knockout mice significantly reduced the area of atherogenic lesions developed in the aorta and aortic sinus. The major components inhibiting leukocyte-type 12-lipoxygenase contained in guava leaf extracts were identified as ethyl gallate and quercetin.

Research Products

• [Journal Article] Gastrointestinal Digestion and Absorption of Pen j 1, a Major Allergen from Kuruma Prawn, Penaeus japonicas. (2011)
  • Author(s): Kunimoto A, Yokoro M, Murota K, Yamanishi R, Suzuki-Yamamoto T, Suzuki M, Yutani C, Doi S, Hiemori M, Yamashita H, Takahashi Y, Tsuji H, Kimoto M.
  • Journal Title: Biosci. Biotechnol. Biochem in press
• [Journal Article] Functional expression of single-chain antibody to leukotriene C(4) (2010)
  • Author(s): Kawakami Y, Yamashita C, Kashiwase Y, Morinaka T, Suzuki-Yamamoto T, Yamashita H, Kimoto M, Tsuji H, Kurahashi Y, Daiyasu H, Toh H, Sugahara M, Miyano M, Yamamoto S, Takahashi Y.
  • Journal Title: Biochem Biophys Res Commun 392 Pages: 421-425
• [Journal Article] Preparation and epitope mapping of a monoclonal antibody against Tri a Bd 27K, a wheat major allergen. (2009)
  • Author(s): Nobuko Komiyama, Miki Hiemori, Masumi Kimoto, Makiko Suzuki, Hiromi Yamashita, Yoshitaka Takahashi, Hideaki Tsuji
  • Journal Title: Biosci. Biotechnol. Biochem 73 Pages: 2113-2116
• [Journal Article] Tumor-suppressive lipoxygenases inhibit the expression of c-myc mRNA coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein 1 in human prostate carcinoma PC-3 cells. (2009)
  • Author(s): Kawakami Y, Kubota N, Ekuni N, Suzuki-Yamamoto T, Kimoto M, Yamashita H, Tsuji H, Yoshimoto T, Jisaka M, Tanaka J, Fujimura HF, Miwa Y, Takahashi Y.
  • Journal Title: Biosci. Biotechnol. Biochem 73 Pages: 1811-1817
  • NAID: 10027544597
• [Journal Article] Antiproliferative activity of guava leaf extract via inhibition of prostaglandin endoperoxide H synthase isoforms.Prostaglandins (2009)
  • Author(s): Kawakami Y, Nakamura T, Hosokawa T, Suzuki-Yamamoto T, Yamashita H, Kimoto M, Tsuji H, Yoshida H, Hada H, Takahashi Y.
  • Journal Title: Leukotrienes and Essential Fatty Acids 80 Pages: 239-245
• [Journal Article] Effects of acetate on lipid metabolism in muscles and adipose tissues of type 2 diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats (2009)
  • Author(s): Yamashita H, Maruta H, Jozuka M, Kimura R, Iwabuchi H, Yamato M, Saito T, Fujisawa K, Takahashi Y, Kimoto M, Hiemori M, Tsuji H.
  • Journal Title: Biosci Biotechnol Biochem 73 Pages: 570-576
  • NAID: 10027538406
• [Journal Article] Antiproliferative activity of guava leaf extract via inhibition of prostaglandin endoperoxide H synthase isoforms (2009)
  • Author(s): Yuki Kawakami
  • Journal Title: Prostaglandins Leukot Essent Fatty Acids 80 Pages: 239-245
  • Peer Reviewed
• [Journal Article] 動脈硬化を予防するグアバ茶の開発に向けた基礎的研究 (2008)
  • Author(s): 高橋 吉孝
  • Journal Title: 平成19年度特別研究報告書 Pages: 39-44
• [Presentation] グァバ葉抽出物に含まれる没食子酸エチルによる白血球型12-リポキシゲナーゼ阻害 (2011)
  • Author(s): 川上祐生
  • Organizer: 第65回日本栄養・食糧学会
  • Place of Presentation: 東京
• [Presentation] 動脈硬化ならびに関連疾患の予防法確立を目指した研究 (2011)
  • Author(s): 高橋吉孝
  • Organizer: OPUフォーラム2011
  • Place of Presentation: 岡山
• [Presentation] 抗ロイコトリエンモノクローナル抗体の構造解析に関する研究 (2011)
  • Author(s): 川上祐生
  • Organizer: OPUフォーラム2011
  • Place of Presentation: 岡山
• [Presentation] Identification of leukocyte-type 12-lipoxygenase inhibitor in guava leaf extract. Bioactive Okayama 2010 (2010)
  • Author(s): 高橋吉孝
  • Organizer: International Conference on Biologically-Active Substances
  • Place of Presentation: Okayama
• [Presentation] Functional expression and site-directed mutagenesis of single-chain antibody to leukotorien C4 (2010)
  • Author(s): 川上祐生
  • Organizer: Bioactive Okayama 2010 International Conference on Biologically-Active Substances
  • Place of Presentation: Okayama
• [Presentation] 抗ロイコトリエンC4単鎖抗体のNusA 融合タンパク質としての発現とロイコトリエン中和作用 (2010)
  • Author(s): 川上祐生
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会・合同大会
  • Place of Presentation: 神戸
• [Presentation] 大豆イソフラボンのシクロオキシゲナーゼ活性調節作用に関する研究 (2010)
  • Author(s): 川上祐生
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 伊香保
• [Presentation] 生活習慣病の早期発見と予防に向けた研究 (2010)
  • Author(s): 高橋吉孝
  • Organizer: OPUフォーラム2010
  • Place of Presentation: 岡山
• [Presentation] 気管支喘息の改善を目指した抗ロイコトリエン抗体に関する研究 (2010)
  • Author(s): 川上祐生
  • Organizer: OPUフォーラム2010
  • Place of Presentation: 岡山
• [Presentation] 大豆イソフラボンのシクロオキシゲナーゼ活性調節作用に関する研究 (2010)
  • Author(s): 川上祐生
  • Organizer: 不二たん白質研究振興財団第13回研究報告会
  • Place of Presentation: 大阪
• [Presentation] グァバ葉抽出物に含まれる白血球型12-リポキシゲナーゼ阻害物質の探索 (2010)
  • Author(s): 川上祐生
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 伊香保
• [Presentation] Identification of leukocyte-type 12-lipoxygenase inhibitor in guava leaf extract (2010)
  • Author(s): 高橋吉孝
  • Organizer: Bioactive Okayama 2010 International Conference on Biologically-Active Substances
  • Place of Presentation: 岡山
• [Presentation] 炎症の予防・改善を可能にするグアバ葉抽出物の開発を目指した研究 (2009)
  • Author(s): 川上 祐生
  • Organizer: 第13回岡山リサーチパーク研究・展示発表会
  • Place of Presentation: 岡山
  • Year and Date: 2009-02-06
• [Presentation] グアバポリフェノールの機能性に関する研究 (2009)
  • Author(s): 細川 朋子
  • Organizer: おかやまバイオアクテイブ研究会 第33回シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2009-01-13
• [Presentation] Induction of low density lipoprotein receptor in HepG2 cells by tocotrienols. (2009)
  • Author(s): 川上祐生
  • Organizer: Fifth Woosong University-Okayama Prefectural University Joint Conference on Nutrition.
  • Place of Presentation: Chengdu, China
• [Presentation] Effect of guava leaf extract on leukocyte-type 12-lipoxygenase activity. (2009)
  • Author(s): 川上祐生
  • Organizer: The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai.
  • Place of Presentation: Sendai
• [Presentation] 脂質異常症の病態 (2009)
  • Author(s): 高橋吉孝
  • Organizer: 21年度岡山県栄養士会生涯学習研修会講演
  • Place of Presentation: 岡山
• [Presentation] 部位特異的変異体を用いた抗ロイコトリエンC4モノクローナル抗体の抗原認識部位の解析 (2009)
  • Author(s): 川上祐生
  • Place of Presentation: 名古屋
• [Presentation] 抗ロイコトリエンC4抗体の単鎖抗体による発現 (2009)
  • Author(s): 川上祐生
  • Organizer: OPUフォーラム2009
  • Place of Presentation: 岡山
• [Presentation] 白血球型12-リポキシゲナーゼに対するグァバ葉抽出物の阻害効果 (2009)
  • Author(s): 川上祐生
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎
• [Presentation] 大豆イソフラボンのシクロオキシゲナーゼ活性阻害作用 (2009)
  • Author(s): 川上祐生
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
• [Presentation] 炎症の予防・改善を可能にするグァバ葉抽出物の開発 (2009)
  • Author(s): 川上祐生
  • Organizer: 第13回岡山リサーチパーク研究・展示発表会
  • Place of Presentation: 岡山
• [Presentation] グァバ葉抽出物による12-リポキシゲナーゼ阻害を介した動脈硬化抑制 (2009)
  • Author(s): 川上祐生
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Effect of guava leaf extract on leukocyte-type 12-lipoxygenase activity (2009)
  • Author(s): 川上祐生
  • Organizer: The 1st International Conference on Lipid Hydroperoxide Biology and Medicine
  • Place of Presentation: 仙台
• [Presentation] グアバ葉抽出物による白血球型12-リポキシゲナーゼの阻害 (2008)
  • Author(s): 細川 朋子
  • Organizer: 第31回日本分子生物学会年回第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Inhibition of leukocyte-type 12-lipoxygenase by the polyphenols from Psidium guajava (2008)
  • Author(s): Tomoko Hosokawa
  • Organizer: Fourth Woosong University-Okayama Prefectural University Joint Conference on Nutrition
  • Place of Presentation: Daejeon, Korea
  • Year and Date: 2008-09-21
• [Presentation] グァバ葉抽出物による白血球型12-リポキシゲナーゼの阻害 (2008)
  • Author(s): 川上祐生
  • Organizer: 第31回日本分子生物学会年回・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] cDNA Cloning and Expression of a Mouse Anti-LTC4 Monoclonal Antibody. (2008)
  • Author(s): 川上祐生
  • Organizer: Fourth Woosong University-Okayama Prefectural University Joint Conference on Nutrition.
  • Place of Presentation: Daejeon, Korea
• [Presentation] 抗ロイコトリエンC4モノクローナル抗体のcDNAクローニングと発現 (2008)
  • Author(s): 川上祐生
  • Organizer: 第50回日本脂質生化学会
  • Place of Presentation: 徳島
• [Presentation] ポリフェノールの生活習慣病予防への応用を目指したアラキドン酸代謝に関する基礎研究 (2008)
  • Author(s): 高橋吉孝
  • Organizer: 第4回「酢の機能性活用コンソーシアム」研究発表・セミナー
  • Place of Presentation: 岡山
• [Presentation] グアバ葉ポリフェノールの抗炎症効果 (2008)
  • Author(s): 川上祐生
  • Organizer: 日本農芸化学学会主催 2008年度大会
  • Place of Presentation: 名古屋
• [Book] エイコサノイドと関連化合物 (1)生合成と生理作用 (中野・ほか編,ビタミン総合辞典 (2010)
  • Author(s): 高橋吉孝
  • Publisher: 朝倉書店
• [Book] 栄養科学シリーズNEXT 「基礎有機化学」 (2010)
  • Author(s): 高橋吉孝・辻英明編
  • Total Pages: 148
  • Publisher: 講談社サイエンティフィク