Studies on the biochemical mechanism of N_2O generation by marine nitrifying bacterium

• Project/Area Number: 20510007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental dynamic analysis
• Research Institution: Shizuoka University
• Principal Investigator: FUJIWARA Taketomo Shizuoka University, 理学部, 教授 (80209121)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 地球温暖化 / 亜酸化窒素 / 温室効果ガス / 一酸化窒素 / 硝化細菌 / 海洋

Research Abstract

Hydroxylamine oxidoreductase (HAO) was purified from marine ammonia-oxidizing bacterium (AOB) strain NS58. Generation of N_2O as a by-product of HAO reaction was evidenced by in vitro experiment using the purified enzyme. Unique isotopic property of the N_2O generated by HAO reaction was also revealed. Molecular and enzymatic features of nitrite reductase, that was involved in the nitrifier denitrification pathway (another N_2O generation mechanism in AOB), was investigated using the recombinant enzyme.

Research Products

• [Journal Article] Mechanism of peptide modification by low-temperature microwave plasma. (2011)
  • Author(s): Motrescu I, Ogino A, Tanaka S, Fujiwara T, et al.
  • Journal Title: Soft Matter accepted.
  • Peer Reviewed
• [Journal Article] Mechanism of peptide modification by low-temperature microwave plasma. (2011)
  • Author(s): I.Motrescu, A.Ogino, S.Tanaka, T.Fujiwara, S.Kodani, H.Kawagishi, G.Popa, M.Nagatsu
  • Journal Title: Soft Matter Volume: (Accepted)
  • Peer Reviewed
• [Journal Article] Effect of salinity on hydroxylamine oxidation in marine ammonia-oxidizing γ-proteobacterium, Nitrosococcus oceani : molecular and catalytic properties of tetraheme cytochrome c-554 (2010)
  • Author(s): Hozuki T, Ohtsuka T, Arai K, Yoshimatsu K, Tanaka S, Fujiwara T.
  • Journal Title: Microbe.Environ. 25 Pages: 95-102
  • Peer Reviewed
• [Journal Article] Modification of peptide by surface wave plasma processing. (2010)
  • Author(s): Motrescu I, Ogino A, Tanaka S, Fujiwara T, et al.
  • Journal Title: Thin Soild Films 518 Pages: 3585-3589
  • Peer Reviewed
• [Journal Article] Effect of Salinity on Hydroxylanmine Oxidation in a Marine Ammonia-Oxidizing Gammaproteobacterium, nitrosococcus ocearistrain NS58 : Molecular and Catalytic Properties of Tetraheme Cytochrome c-554. (2010)
  • Author(s): Hozuki T, Ohtsuka T, Arai K, Yoshimatsu K, Tanaka S, Fujiwara, T.
  • Journal Title: Microb.Environ. Volume: 25 Pages: 95-102
  • Peer Reviewed
• [Journal Article] Nitrogen cycle in the extremely halophilic environment biochemistry of haloerchaeal deritrification Reviewin Japanese. (2010)
  • Author(s): Yoshimatsu K, Fujiwara T.
  • Journal Title: Seikagaku Volume: 81 Pages: 1087-1093
• [Journal Article] Modification of Peptide by Surface Wave Plasma Processing (2010)
  • Author(s): I.Motrescu, A.Ogino, S.Tanaka, T.Fujiwara, S.Kodani, H.Kawagishi, G.Popa, M.Nagatsu
  • Journal Title: Thin Solid Films Volume: 518 Pages: 3585-3589
  • Peer Reviewed
• [Journal Article] Effect of Salinity on Hydroxylamine Oxidation in a Marine Ammonia-Oxidizing Gammaproteobacterium, Nitrosococcus oceani strain NS58 : Molecular and Catalytic Properties of Tetraheme Cytochrome c-554 (2010)
  • Author(s): Hozuki T, Ohtsuka T, Arai K, 吉松勝彦, Tanaka S, 藤原健智
  • Journal Title: Microb.Environ. (In press)
  • NAID: 10030689091
  • Peer Reviewed
• [Journal Article] Modification of peptide by surface wave plasma processing (2010)
  • Author(s): Motrescu I, Ogino A, Tanaka S, 藤原健智, Kodani S, Kawagishi H, Popa G, Nagatsu M
  • Journal Title: Thin Soild Films (In press)
  • Peer Reviewed
• [Journal Article] 高度好塩環境における窒素循環, 特に脱窒作用に関する生化学 (2010)
  • Author(s): 吉松勝彦, 藤原健智
  • Journal Title: 生化学 81(12) Pages: 1087-1093
  • NAID: 10026299946
• [Journal Article] Invetigation of low temperature plasma capabilities to modify the structure and function of bio-polymers. (2009)
  • Author(s): Motrescu I, Hara T, Ogino A,Tanaka S, Fujiwara T, et al.
  • Journal Title: J Automation, Mobile Robotics and Intelligent Systems 3 Pages: 150-152
  • Peer Reviewed
• [Journal Article] Invetigation of low temperature plasma capabilities to modify the structure and function of bio-polymers (2009)
  • Author(s): Motrescu I, Hara T, Ogino A, Tanaka S, 藤原健智, Kawagishi H, Kodani S, Popa G, Nagatsu M
  • Journal Title: J Automation, Mobile Robotics and Intelligent Systems 3 Pages: 150-152
  • Peer Reviewed
• [Journal Article] Bacillus polygonumi sp.nov., moderate halophilic non-motile obligate alkaliphile isolated from indigo ball. (2008)
  • Author(s): Aino K, Hirota K, Matsuno T, Naoki Morita N, Nodasaka Y, Fujiwara T, et al.
  • Journal Title: Int.J.Syt.Evol.Microbiol. 58(1) Pages: 120-124
  • Peer Reviewed
• [Journal Article] Bacillus polygonumi sp. nov., moderate halophilic non-motile obligate alkaliphile isolated from indigo ball (2008)
  • Author(s): Aino K, Hirota K, Matsuno T, Naoki Morita N, Nodasaka Y, Fujiwara T, Matsuyama H, Yoshimune K, Yumoto I.
  • Journal Title: Int. J. Syt. Evol. Microbiol. 58(1) Pages: 120-124
  • Peer Reviewed
• [Journal Article]
  • Peer Reviewed
• [Presentation] ヒドロキシルアミン酸化酵素による亜酸化窒素生成の分子機構 (2010)
  • Author(s): 保月勇志, 山崎哲明, 吉松勝彦, 吉田尚弘, 藤原健智
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 脱窒性アーキアの硝酸塩還元酵素ハイブリッド複合体の構造と機能 (2010)
  • Author(s): 吉松勝彦、藤原健智
  • Organizer: 第37回生体分子科学討論会
  • Place of Presentation: 山口
• [Presentation] 脱窒性アーキアの硝酸塩還元酵素ハイブリット複合体の構造と機能 (2010)
  • Author(s): 吉松勝彦、藤原健智
  • Organizer: 第37回生体分子科学討論会
  • Place of Presentation: 山口大学吉田キャンパス、大学会館(山口市)
• [Presentation] ヒドロキシルアミン酸化酵素による亜酸化窒素生成の分子機構 (2010)
  • Author(s): 保月勇志、山崎哲明、吉松勝彦、吉田尚弘、藤原健智
  • Organizer: 第33回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
• [Presentation] Molecular and functional analysis of cytochrome c-554 from marine ammonia-oxidizing γ- proteobacteria (2009)
  • Author(s): Hozuki, et al
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 好塩性アーキアの脱窒を制御するDNA結合タンパク質NarRについて (2009)
  • Author(s): 志波宏峻、吉松勝彦、藤原健智
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 好塩性アーキアの新規なヘムタンパク質PitAの構造と機能 (2009)
  • Author(s): 田中達、吉松勝彦、藤原健智
  • Organizer: 第82回日本生化学大会
  • Place of Presentation: 神戸
• [Presentation] 好塩性アーキアの硝酸塩還元酵素ハイブリッド複合体の精製と生化学的性質 (2009)
  • Author(s): 吉松勝彦、藤原健智
  • Organizer: 第82回日本生化学大会
  • Place of Presentation: 神戸
• [Presentation] 好塩性アーキアの新規なヘムタンパク質 PitA の構造と機能 (2009)
  • Author(s): 田中達, 吉松勝彦, 藤原健智
  • Organizer: 第82回日本生化学大会
  • Place of Presentation: 神戸国際会議場 (神戸市)
• [Presentation] 好塩性アーキアの脱窒を制御するDNA結合タンパク質NarRについて (2009)
  • Author(s): 志波宏峻, 吉松勝彦, 藤原健智
  • Organizer: 第82回日本生化学大会
  • Place of Presentation: 神戸国際会議場 (神戸市)
• [Presentation] 好塩性アーキアの硝酸塩還元酵素ハイブリッド複合体の精製と生化学的性質 (2009)
  • Author(s): 吉松勝彦, 藤原健智
  • Organizer: 第82回日本生化学大会
  • Place of Presentation: 神戸国際会議場 (神戸市)
• [Presentation] 海洋性γ-アンモニア酸化細菌の cytochrome c-554の構造と機能 (2009)
  • Author(s): 保月勇志, 吉松勝彦, 藤原健智
  • Organizer: 第82回日本生化学大会
  • Place of Presentation: 神戸国際会議場 (神戸市)
• [Presentation] 好塩性アーキアの硝酸塩還元酵素オペロンにコードされるRieske型鉄硫黄タンパク質NarBの機能 (2008)
  • Author(s): 吉松勝彦, 藤原健智
  • Organizer: BMB2008
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-11
• [Presentation] Ammonia monooxygenase activity of marine gamma- proteobacterium NS58 isolated from anoxic coastal sediment. (2008)
  • Author(s): Arai, K., Y.Katsuhiko, T.Fujiwara
  • Organizer: The 7th International Symposium for Subsurface Microbiology
  • Place of Presentation: Shizuoka
• [Presentation] Ammonia monooxygenase activity of marine gamma-proteobacterium NS58 isolated from anoxic coastal sediment (2008)
  • Author(s): Arai K, Yoshimatsu K, Fujiwara T.
  • Organizer: 7th International Symposium for Subsurface Microbiology ISSM2008
  • Place of Presentation: Shizuoka
• [Book] biochemistry of haloarchaeal denitrification(Nitrogen cycle in the extremely halophilic environment)(Seikagaku81(12)) (2009)
  • Author(s): Yoshimatsu K, Fujiwara T.
  • Publisher: Review in Japanese.
• [Book] Development of halophilic protein expression system capable of creating a productive enzyme.Noda Inst.Sci.Res.Grant Rep.53 (2009)
  • Author(s): 藤原健智
• [Book] 酵素ハンドブック第3版 (2008)
  • Author(s): 藤原健智(共著)
  • Publisher: 朝倉書店
• [Remarks] ホームページ等(研究室ホームページ)
  • URL: http://www.ipc.shizuoka.ac.jp/~sbtfuji/TF-Lab-J.html
• [Remarks]
  • URL: http://www.ipc.shizuoka.ac.jp/%7esbtfuji/TF-Lab-J.html
• [Remarks]
  • URL: http://www.ipc.shizuoka.ac.jp/%7esbtfuji/TF-Lab-J.html