The development of the influence evaluation methods exerted on the ecosystem of Japanese coastal environment plasmids in the petroleum contamination

• Project/Area Number: 20510021
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental impact assessment/Environmental policy
• Research Institution: Ibaraki University
• Principal Investigator: KURUSU Yasurou Ibaraki University, 農学部, 教授 (60272118)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 環境プラスミド / 安定分配 / 芳香族炭化水素分解菌 / 脂肪族炭化水素分解菌 / プラスミド / Sphingomonad / Pseudomonas / 炭化水素分解遺伝子 / 16SrRNA / 石油分解菌 / フェナントレン / C-重油

Research Abstract

The plasmids from petroleum-degradation bacteria were searched in this research. Many plasmids were discovered from above bacteria that isolated from two points, and all were giant plasmids. It was suggested that horizontal transfer of a group of petroleum-degradation genes occurred by conjugation between environmental microorganisms. Moreover, the novel genes concerned with the plasmid stability were identified from two plasmids.

Research Products

• [Presentation] 海洋性Sphingomonas属細菌を宿主とする遺伝子組換え系の開発 (2010)
  • Author(s): 林宏恵、久留主泰朗
  • Organizer: 第13回マリンバイオテクノロジー学会年会
  • Place of Presentation: 広島大学
• [Presentation] Construction of a high-expression system of foreign genes in Sphingomonad (2010)
  • Author(s): Hayashi.H, Kurusu.Y
  • Organizer: ASM General 110^<th> Meeting
  • Place of Presentation: San Diego, USA
• [Presentation] 海洋性Sphingomonas属細菌を宿主とする遺伝子組換え系の開発 (2010)
  • Author(s): 林宏恵、久留主泰朗
  • Organizer: 第13回マリンバイオテクノロジー学会年会
  • Place of Presentation: 東広島市、広島大学
• [Presentation] Sphingomonas細菌由来プラスミドの宿主内分配機構の解析 (2009)
  • Author(s): 大森孟道、雄長誠、林宏恵、久留主泰朗
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Sphingomonas細菌群を宿主とする高発現ベクターの構築 (2009)
  • Author(s): 福田敦史、林宏恵、久留主泰朗
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Characterization of Novel Plasmids from Sphingomonad (2009)
  • Author(s): Kurusu Y, Ochou M, Saito M
  • Organizer: ASM General 109^<th> Meeting
  • Place of Presentation: Philadelphia, USA
• [Presentation] Sphingomonas 属細菌群を宿主とする高発現ベクターの構築 (2009)
  • Author(s): 福田敦史, 林宏恵, 久留主泰朗
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市、パシフィコ横浜
• [Presentation] Sphingomonas 属細菌由来プラスミドの宿主内分配機構の解析 (2009)
  • Author(s): 大森孟道, 雄長誠, 林宏恵, 久留主泰朗
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市、パシフィコ横浜
• [Presentation] 日本周辺海洋環境からの石油分解菌の探索とプラスミド解析 (2008)
  • Author(s): 菊地真由美,関香織,西村彰浩,久留主泰朗, 他
  • Organizer: 日本微生物生態学会第24回大会
  • Place of Presentation: 北大
  • Year and Date: 2008-11-26
• [Presentation] 日本周辺海洋環鏡からの石油分解菌の探索とプラスミド解析 (2008)
  • Author(s): 菊地真由美, 関香織, 西村彰浩, 久留主泰朗, その他3名
  • Organizer: 日本微生物生態学会第24回大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2008-11-26
• [Remarks] ホームページ等
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