Characterization and biological assessment of DNA-Protein crosslink repair in vertebrate cells

• Project/Area Number: 20510051
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: TANO Keizo Kyoto University, 原子炉実験所, 准教授 (00183468)
• Co-Investigator(Kenkyū-buntansha): KASHINO Genro 京都大学, 原子炉実験所, 助教 (00437287) ; MASUNAGA Shinichiro 京都大学, 原子炉実験所, 准教授 (80238914)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 修復 / 生物影響

Research Abstract

To elucidate the process of DNA-Protein Crosslink (DPC) repair, we employed reverse genetic approach using chicken DT40 cells. It has been suggested that endogenous reactive oxygen species (ROS) is involved in formation of DPC. We generated several ROS scavenge enzyme genes knock out cells to elucidate mechanism of antioxidant defense system. In addition, we try to look for the therapeutic potential of DPC producing chemical, HMTA, in mouse level. All the results so far, already published in peer reviewed journals.

Research Products

• [Journal Article] An alternative mechanism for radioprotection by dimethyl sulfoxide ; possible facilitation of DNA double-strand break repair (2010)
  • Author(s): Kashino G, Liu Y, Suzuki M, Masunaga S, Kinashi Y, Ono K, Tano K, Watanabe M
  • Journal Title: J.Radiat. Res. 51 Pages: 733-740
  • NAID: 10026964239
  • Peer Reviewed
• [Journal Article] SOD1 Is Essential for the Viability of DT40 Cells and Nuclear SOD1 Functions as a Guardian of Genomic DNA (2010)
  • Author(s): Inoue E, Tano K, Yoshii H, Nakamura J, Tada S, Watanabe M, Seki M, Enomoto T
  • Journal Title: J.Nucleic Acids 795946 Pages: 1-11
  • Peer Reviewed
• [Journal Article] FEN1 Functions in Long Patch Base Excision Repair Under Conditions of Oxidative Stress in Vertebrate Cells (2010)
  • Author(s): Asagoshi K, Tano K, ChastainII PD, Adachi N, Sonoda E, Kikuchi K, Koyama H, Nagata K, Kaufman DG, Takeda S, Wilson SH, Watanabe M, Swenberg JA, Nakamura J
  • Journal Title: Mol.Cancer Res. 8 Pages: 204-215
  • Peer Reviewed
• [Journal Article] Adverse effect of mild hyperthermia combined with hexyamethylenetetramine compared to its effect combined with tirapazamine in the treatment of solid tumors (2010)
  • Author(s): Masunaga S, Tano K, Nakamura J, Watanabe M, Kashino G, Suzuki M, Kinashi Y, Ono K
  • Journal Title: Exp.Ther.Med. 1 Pages: 169-174
  • Peer Reviewed
• [Journal Article] Usefulness of hexamethylenetetramine as an adjuvant to radiation and cisplatin in the treatment of solid tumors : its independency of p53 status (2010)
  • Author(s): Masunaga S, Tano K, Nakamura J, Watanabe M, Kashino G, Takahashi A, Tanaka H, Suzuki M, Ohnishi K, Kinashi Y, Liu Y, Ohnishi T, Ono K
  • Journal Title: J.Radiat. Res. 51 Pages: 27-35
  • NAID: 10025914038
  • Peer Reviewed
• [Journal Article] SOD1 Is Essential for the Viability of DT40 Cells and Nuclear SOD1 Functions as a Guardian of Genomic DNA (2010)
  • Author(s): Inoue E, Tano K, Yoshii H, Nakamura J, Tada S, Watanabe M, Seki M, Enomoto T
  • Journal Title: Journal of Nucleic Acids Volume: 2010
  • Peer Reviewed
• [Journal Article] An alternative mechanism for radioprotection by dimethyl sulfoxide ; possible facilitation of DNA double-strand break repair (2010)
  • Author(s): Kashino G, Liu Y, Suzuki M, Masunaga S, Kinashi Y, Ono K, Tano K, Watanabe M
  • Journal Title: Journal of Radiation Research Volume: 51 Pages: 733-740
  • NAID: 10026964239
  • Peer Reviewed
• [Journal Article] Usefulness of hexamethylenetetramine as an adjuvant to radiation and cisplatin in the treatment of solid tumors : its independency of p53 status (2010)
  • Author(s): Masunaga S, Tano K, Nakamura J, Watanabe M, Kashino G, Takahashi A, Tanaka H, Suzuki M, Ohnishi K, Kinashi Y, Liu Y, Ohnishi T, Ono K
  • Journal Title: J.Radiat.Res. 51 Pages: 27-35
  • NAID: 10025914038
  • Peer Reviewed
• [Journal Article] Cells deficient in PARP-1 show an accelerated accumulation of DNA single strand breaks, but not AP sites, over the PARP-1-proficient cells exposed to MMS (2009)
  • Author(s): Pachkowski BF, Tano K, Afonin V, Elder RH, Takeda S, Watanabe M, Swenberg JA, Nakamura J
  • Journal Title: Mut.Res. 671 Pages: 93-99
  • Peer Reviewed
• [Journal Article] Evaluation of the potential of hexamethylenetetramine, compared with tirapazamine, as a combined agent with γ-irradiation and cisplatin treatment in vivo (2009)
  • Author(s): Masunaga S, Tano K, Watanabe M, Kashino G, Suzuki M, Kinashi Y, Ono K, Nakamura J
  • Journal Title: Br.J.Radiol. 82 Pages: 392-400
  • Peer Reviewed
• [Journal Article] Usefulness of hexamethylenetetramine in combination with chemotherapy using free and pegylated liposomal doxorubicin in vivo, referring to the effect on quiescent cells (2009)
  • Author(s): Masunaga S, Kono K, Nakamura J, Tano K, Yoshida H, Watanabe M, kahino G, Suzuki M, Kinashi Y, Ono K
  • Journal Title: Oncol. Rep. 21 Pages: 1307-1312
  • Peer Reviewed
• [Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2 (2009)
  • Author(s): Takada S, Inoue E, Tano K, Yoshii H, Abe T, Yoshimura A, Akita M, Tada S, Watanabe M, Seki M, Enomoto T
  • Journal Title: Biochem. Biophys. Res.Commun. 379 Pages: 233-238
  • Peer Reviewed
• [Journal Article] Evaluation of the potential of hexamethylenetetramine, compared with tirapazamine, as a combined agent with γ-irradiation and cisplatin treatment in vivo (2009)
  • Author(s): S. Masunaga, K. Tano, M. Watanabe, G. Kashino, M. Suzuki, Y. Kinashi, K. Ono, J. Nakamura
  • Journal Title: British Journal of Radiology
  • Peer Reviewed
• [Journal Article] Usefulness of hexamethylenetetramine in combination with chemotherapy using free and pegylated liposomal doxorubicin in vivo, referring to the effect on quiescent cells (2009)
  • Author(s): S. Masunaga, K. Kono, J. Nakamura, K. Tano, H. Yoshida, M. Watanabe, G. kahino, M. Suzuki, Y. Kinashi, K. Ono
  • Journal Title: Oncology Reports
  • Peer Reviewed
• [Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2 (2009)
  • Author(s): S. Takada, E. Inoue, K. Tano, H. Yoshii, T. Abe, A. Yoshimura, M. Akita, S. Tada, M. Watanabe, M. Seki, T. Enomoto
  • Journal Title: Biochemical and biophysical research communications 379 Pages: 233-238
  • Peer Reviewed
• [Presentation] Either SOD1, SOD2 or PRDX1 is required for normal cell growth in vertebrate cells (2011)
  • Author(s): 田野恵三, 井上絵里, 吉村明,関政幸, 榎本武美, 渡邉正己
  • Organizer: The 2011 GRC on Oxidative Stress and Disease
  • Place of Presentation: Ventura, CA, USA.
  • Year and Date: 2011-03-16
• [Presentation] Either SOD1, SOD2 or PRDX1 is required for normal cell growth in vertebrate cells (2011)
  • Author(s): Tano K, Inoue E, Yoshimura A, Seki M, Enomoto T, Watanabe M
  • Organizer: The 2011 GRC on Oxidative Stress and Disease
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 2011-03-16
• [Presentation] OD1枯渇細胞とPRDX1枯渇細胞の抗酸化剤に対する挙動の違い (2010)
  • Author(s): 樋口優人、井上絵里、田野恵三、吉村明、渡邉正己、榎本武美、関政幸
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] SOD1枯渇細胞とPRDX1枯渇細胞の抗酸化剤に対する挙動の違い (2010)
  • Author(s): 樋口優人, 井上絵里, 田野恵三, 吉村明, 渡邉正己, 榎本武美, 関政幸
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] 染色体3倍体化におけるがん形質の獲得 (2010)
  • Author(s): 縄田寿克、菓子野元郎、田野恵三,法村俊之、渡邉正己
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-20
• [Presentation] 細胞内局在性を異にする内在性スーパーオキシドによる細胞増殖阻害へのアスコルビン酸及び低酸素の効果 (2010)
  • Author(s): 田野恵三、井上絵里、縄田寿克、菓子野元郎、関政幸、榎本武美、渡邉正己
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-20
• [Presentation] X線誘発微小核形成はDNA二重鎖切断に起因しない (2010)
  • Author(s): 岡田卓也、菓子野元郎、田野恵三、渡邉正己
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-20
• [Presentation] X線誘発微小核形成はDNA 二重鎖切断に起因しない (2010)
  • Author(s): 岡田卓也, 菓子野元郎, 田野恵三, 渡邉正己
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-20
• [Presentation] 染色体3倍体化おけるがん形質の獲得 (2010)
  • Author(s): 縄田寿克, 菓子野元郎, 田野恵三, 法村俊之, 渡邉正己
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-20
• [Presentation] Nuclear superoxide may trigger spontaneous sister chromatid exchanges (2009)
  • Author(s): 井上絵里、田野恵三、吉居華子、高田隼也、吉村明、縄田寿克、中村純、多田周右、渡邉正己、関政幸、榎本武美
  • Organizer: 日本分子生物学会32回年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Nuclear superoxide may trigger spontaneous sister chromatid exchanges (2009)
  • Author(s): 井上絵里, 田野恵三, 吉居華子, 高田隼也, 吉村明, 縄田寿克, 中村純, 多田周右, 渡邉正己, 関政幸, 榎本武美
  • Organizer: 日本分子生物学会32回年回
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] 発がん過程における染色体異数化の役割 (2009)
  • Author(s): 縄田寿克、田野恵三、久郷裕之、押村光雄、渡邉正己
  • Organizer: 日本放射線影響学会52回大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-11
• [Presentation] ビタミンCはX線誘発DNA二重鎖生成を抑制しない (2009)
  • Author(s): 岡田卓也、菓子野元郎、田野恵三、渡邉正己
  • Organizer: 日本放射線影響学会52回大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-11
• [Presentation] 内在性スーパーオキシドによる細胞増殖阻害及び染色体不安定化に対するアスコルビン酸の抑制効果 (2009)
  • Author(s): 田野恵三、井上絵里、吉居華子、縄田寿克、関政幸、榎本武美、渡邉正己
  • Organizer: 日本放射線影響学会52回大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-11
• [Presentation] メラノジェネシスにおける紫外線誘発酸化ストレスの影響 (2009)
  • Author(s): 西浦英樹, 田野恵三, 渡邉正己
  • Organizer: 日本放射線影響学会52回大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-11
• [Presentation] トリ細胞におけるミスマッチ修復遺伝子の紫外線損傷修復に対する機能 (2008)
  • Author(s): 田野恵三, 森俊雄, 中村純, 荒川央, 武田俊一, 渡邉正巳
  • Organizer: 放射線影響学会第51回大会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2008-11-20