| Project/Area Number |
20510054
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KUBO Kihei Osaka Prefecture University, 大学院・生命環境科学研究科, 教授 (40117619)
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| Co-Investigator(Kenkyū-buntansha) |
MATUYAMA Satoshi 大阪府立大学, 大学院・生命環境科学研究科, 准教授 (10254442)
TAKENAKA Shigeo 大阪府立大学, 大学院・生命環境科学研究科, 准教授 (10280067)
YAMAMOTO Ryouhei 大阪府立大学, 大学院・生命環境科学研究科, 助教 (20457998)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 塩基除去修復 / AP部位 / ARP法 / FARP法 / 細胞周期 / グリコシラーゼ / RNAi / DAN polymeraseβ / 脱塩基部位 / FARP-1 / ARP / ノックアウトMEF / ノックダウン / 修復酵素 / 放射線 / 化学物質 / shRNA / 蛍光ARP / snRNA |
|---|
| Research Abstract |
The cell cycle dependent base excision repair of methylated base damage was investigated using a novel assay system for the detection of intranuclear abasic sites. In polβ-KO MEF, the removal of methylated bases was significantly slower than wild type especially in G1 phase, suggesting the lack suppressed the glycosylase activity to bypass the accumulation of hazardous intermediate, AP sites.
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