| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Research Abstract |
Artemis is a nuclease that involves in the repair of DNA double stranded breaks (DSBs). We have analyzed the dynamics of Artemis using live cell imaging methods. After laser microbeam irradiation, human Artemis-GFP fusion proteins were accumulated at DNA damaged sites. By mutational analysis, the multiple phosphorylation sites, but not nuclease domain of Artemis required for its accumulation. Consistent with these observations, the accumulation of Artemis at DNA damaged sites markedly reduced in caffeine treated cells. Collectively, these results suggested that phosphorylation of Artemis regulates its dynamics upon DSBs.
|
