Analysis of nuclear dynamics of Artemis upon DNA-double stranded breaks
| Project/Area Number |
20510057
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
ISHIAI Masamichi Kyoto University, 放射線生物研究センター, 准教授 (90298844)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | DNA損傷 / DNA修復 / レーザー照射 / 生細胞イメージング / リン酸化 / レーザーマイクロ照射法 / 紫外線レーザーマイクロ照射法 |
|---|
| Research Abstract |
Artemis is a nuclease that involves in the repair of DNA double stranded breaks (DSBs). We have analyzed the dynamics of Artemis using live cell imaging methods. After laser microbeam irradiation, human Artemis-GFP fusion proteins were accumulated at DNA damaged sites. By mutational analysis, the multiple phosphorylation sites, but not nuclease domain of Artemis required for its accumulation. Consistent with these observations, the accumulation of Artemis at DNA damaged sites markedly reduced in caffeine treated cells. Collectively, these results suggested that phosphorylation of Artemis regulates its dynamics upon DSBs.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway.2008
Author(s)
Ishiai M., Kitao H., Smogorzewska A., Tomida J., Kinomura A., Uchida E., Saberi A., Kinoshita E., Kinoshita-Kikuta E., Koike T., Tashiro S., Elledge S.J., Takata M.
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Journal Title
Nature Struct. Mol.Biol. Vol.15, No.11
Pages: 1138-1146
Related Report
Peer Reviewed
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[Journal Article] Ku70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation.2008
Author(s)
Abe T., Ishiai M., Hosono Y., Yoshimura A., Tada S., Adachi N., Koyama H., Takata M., Takeda S., Enomoto T., Seki M.
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Journal Title
Cell.Signal. Vol.20, No.11
Pages: 1978-1985
Related Report
Peer Reviewed
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