Development of a novel DNA damage sensor mouse model.

• Project/Area Number: 20510059
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: KOIKE Manabu 独立行政法人放射線医学総合研究所, 放射線防護研究センター, 主任研究員 (70280740)
• Co-Investigator(Kenkyū-buntansha): KOIKE Aki 独立行政法人放射線医学総合研究所, 放射線防護研究センター, 准研究員 (50415410)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Ku70 / DSB / GFP / DNA repair / Ku80

Research Abstract

We generated transgenic mice that were overexpressed GFP-Ku70.Furthermore, we confirmed that the GFP-Ku70 is a useful tool as a detection marker of DNA damaged sites in living mouse epithelial cells.

Research Products

• [Journal Article] Establishment of Ku70-deficient lung epithelial cell lines and their hypersensitivity to low-dose X-irradiation (2011)
  • Author(s): Koike M, Yutoku Y, Koike A
  • Journal Title: Journal of Veterinary Medical Science Pages: 549-554
  • NAID: 130000444626
• [Journal Article] Establishment of Ku70-Deficient Lung Epithelial Cell Lines and Their Hypersensitivity to Low-Dose X-Irradiation. (2010)
  • Author(s): Koike M, Yutoku Y, Koike A.
  • Journal Title: Journal of Veterinary Medical Science Volume: in press
  • NAID: 130000444626
  • Peer Reviewed
• [Presentation] エックス線照射したマウス個体におけるH2AXリン酸化修飾変化の組織特異性に関する研究 (2010)
  • Author(s): 菅澤純、湯徳靖友、小池亜紀、小池学
  • Organizer: 第47回アイソトープ・放射線研究発表会
  • Place of Presentation: 日本科学未来館(東京都)
  • Year and Date: 2010-07-09
• [Presentation] 近紫外線レーザーマイクロ照射によるDNA修復酵素の挙動解析 (2009)
  • Author(s): 小池学
  • Organizer: 第4回高崎量子応用シンポジウム
  • Place of Presentation: 高崎シティーギャラリー・コアホール
  • Year and Date: 2009-10-09
• [Presentation] 近紫外線レーザーマイクロ照射によるDNA修復酵素の挙動解析 (2009)
  • Author(s): 小池学
  • Organizer: 第4回 高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎シティギャラリー・コアホール
  • Year and Date: 2009-10-09