The effect of environmental chemicals on the interaction of thyroid hormone receptor and retinoic acid receptor-related orphan receptor (ROR).

• Project/Area Number: 20510061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Gunma University
• Principal Investigator: IWASAKI Toshiharu Gunma University, 大学院・医学系研究科, 講師 (80375576)
• Co-Investigator(Kenkyū-buntansha): KOIBUCHI Noriyuki 群馬大学, 大学院・医学系研究科, 教授 (80234681)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 小脳初代培養細胞 / Purkinje細胞 / 顆粒細胞 / 環境化学物質 / 甲状腺ホルモン / 脳発達抑制 / ROR / オーファン受容体 / 脳発達 / 相互作用

Research Abstract

(1) We investigated the effect of interaction between thyroid hormone receptor (TR) and retinoic acid receptor-related orphan receptor (ROR).
(2) We investigated the effects of Brominated Organic Chemicals (BOCs) such as polybrominated diphenylethers (PBDEs) on TR-related signaling using newly developed primary culture-based assays.

Research Products

• [Journal Article] Disruption of Thyroid Hormone Receptor-Mediated Transcription and Thyroid Hormone-Induced Purkinje Cell Dendrite Arborization by Polybrominated Diphenylethers. (2011)
  • Author(s): Ibhazehiebo K, Iwasaki T, Kimura-Kuroda J, Miyazaki W, Shimokawa N, Koibuchi N
  • Journal Title: Environ Health Perspect. 119 Pages: 168-175
  • Peer Reviewed
• [Journal Article] 1,2,5,6,9,10-α Hexabromo- cyclododecane (HBCD) Impairs Thyroid Hormone-Induced Dendrite Arborization of Purkinje Cells and Suppresses Thyroid Hormone Receptor-Mediated Transcription. (2011)
  • Author(s): Ibhazehiebo K, Iwasaki T, Shimokawa N, Koibuchi N.
  • Journal Title: Cerebellum. 10 Pages: 22-31
  • Peer Reviewed
• [Journal Article] Brain-derived neurotrophic factor -T4+T4 (10-8 M)+T3 (10-8 M) (BDNF) ameliorates the suppression of thyroid hormone-induced granule cell neurite extension by hexabromocyclododecane (HBCD). (2011)
  • Author(s): Ibhazehiebo K, Iwasaki T, Xu M, Shimokawa N, Koibuchi N
  • Journal Title: Neurosci Lett. 93(1-2) Pages: 1-7
  • Peer Reviewed
• [Journal Article] Suppression of thyroid hormone receptor-mediated transcription and disruption of thyroid hormone-induced cerebellar morphogenesis by the polybrominated biphenyl mixture, BP-6. (2011)
  • Author(s): Ibhazehiebo K, Iwasaki T, Okano-Uchida T, Shimokawa N, Ishizaki Y, Koibuchi N
  • Journal Title: Neurotoxicology. 32 Pages: 400-409
  • Peer Reviewed
• [Journal Article] Disruption of Thyroid Hormone Receptor-Mediated Transcription and Thyroid Hormone-Induced Purkinje Cell Dendrite Arborization by Polybrominated Diphenylethers. (2011)
  • Author(s): Ibhazehiebo K
  • Journal Title: Environ Health Perspect Volume: 119 Pages: 168-175
  • Peer Reviewed
• [Journal Article] 1,2,5,6,9,10-α Hexabromocyclododecane (HBCD) Impairs Thyroid Hormnone-Induced Dendrite Arborization of Purkinje Cells and Suppresses Thyroid Hormone Recepter-Mediated Transcrintion. (2011)
  • Author(s): Ibhazehiebo K
  • Journal Title: Cerebellum Volume: 10 Pages: 22-31
  • Peer Reviewed
• [Journal Article] Brain-derived neurotrophic factor (BDNF) ameliorates the suppression of thyroid hormone-induced granule cell neurite extension by hexabromocyclododecane (HBCD). (2011)
  • Author(s): Ibhazehiebo K
  • Journal Title: Neurosci Lett. Volume: 493(1-2) Pages: 1-7
  • Peer Reviewed
• [Journal Article] Suppression of thyroid hormone receptor-mediated transcription and disruption of thyroid hormone-induced cerebellar morphogenesis by the polybrominated biuhenyl mixture, BP-6. (2011)
  • Author(s): Ibhazehiebo K
  • Journal Title: NeurroToxicology Volume: 32 Pages: 400-409
  • Peer Reviewed
• [Journal Article] The effect of hydroxylated polychlorinated biphenyl (OH-PCB) on thyroid hormone receptor (TR)-mediated transcription through native-thyroid hormone response element (TRE). (2010)
  • Author(s): Amano I, Miyazaki W, Iwasaki T, Shimokawa N, Koibuchi N
  • Journal Title: Ind Health. 48 Pages: 115-118
  • NAID: 130004828544
  • Peer Reviewed
• [Journal Article] Hydroxylated PCB induces Ca2+ oscillations and alterations of membrane potential in cultured cortical cells. (2010)
  • Author(s): Londono M, Shimokawa N, Miyazaki W, Iwasaki T, Koibuchi N
  • Journal Title: J Appl Toxicol. 30 Pages: 334-342
  • Peer Reviewed
• [Journal Article] Hydroxylated PCB induces Ca^<2+> oscillations and alterations of membrane potential in cultured cortical cells. (2010)
  • Author(s): Londono M
  • Journal Title: J Appl Toxicol. Volume: 30 Pages: 334-342
  • Peer Reviewed
• [Journal Article] Hydroxylated PCB induces Ca(2+) oscillations and alterationsof membrane potential in cultured cortical cells. (2010)
  • Author(s): Londono M
  • Journal Title: J Appl Toxicol. (In press)
  • Peer Reviewed
• [Journal Article] Polychlorinated Biphenyl(PCB)on Thyroid Hormone Receptor (TR)-mediated Transcription through Native-Thyroid Hormone Response Element(TRE). (2010)
  • Author(s): Amano I
  • Journal Title: Industorial Health. 48 Pages: 115-118
  • Peer Reviewed
• [Journal Article] Activation of aromatase expression by retinoic acid receptor-related orphan receptor (ROR) alpha in breast cancer cells : identification of a novel ROR response element. (2009)
  • Author(s): Odawara H, Iwasaki T, Horiguchi J, Rokutanda N, Hirooka K, Miyazaki W, Koibuchi Y, Shimokawa N, Iino Y, Takeyoshi I, Koibuchi N
  • Journal Title: J Biol Chem. 284 Pages: 17711-17719
  • Peer Reviewed
• [Journal Article] Interaction of full length ROR・to augment TR-mediated transcription. (2009)
  • Author(s): Qiu CH, Miyazaki W, Iwasaki T, Londono M, Ibhazehiebo K, Shimokawa N, Koibuchi N
  • Journal Title: Thyroid. 19 Pages: 893-898
  • Peer Reviewed
• [Journal Article] Activation of aromatase expression by retinoic acid receptor-related orphan receptor(ROR)alpha in breast cancer cells : identification of a novel ROR response element. (2009)
  • Author(s): Odawara H
  • Journal Title: J Biol Chem. 284 Pages: 17711-17719
  • Peer Reviewed
• [Journal Article] Interaction of full length RORα to augment TR-mediated transcription. (2009)
  • Author(s): Qiu C-H
  • Journal Title: Thyroid. 19 Pages: 893-898
  • Peer Reviewed
• [Journal Article] Augmentation of estrogen receptor-mediated transcription by steroid and xenobiotic receptor. (2009)
  • Author(s): Rokutanda N, et al.
  • Journal Title: Endocrine (in press)
  • Peer Reviewed
• [Journal Article] Direct interaction of retinoic acid receptor-related orphan receptor (ROR)α with thyroid hormone receptor (TR) to augment TR-mediated transcription (2009)
  • Author(s): Qiu CH, et al.
  • Journal Title: Thyroid (in press)
  • Peer Reviewed
• [Journal Article] Augmentation of estrogen receptor-mediated transcription by steroid and xenobiotic receptor. (2008)
  • Author(s): Rokutanda N, Iwasaki T, Odawara H, Nagaoka R, Miyazaki W, Takeshita A, Koibuchi Y, Horiguchi J, Shimokawa N, Iino Y, Morishita Y, Koibuchi N
  • Journal Title: Endocrine. 33 Pages: 305-316
  • Peer Reviewed
• [Journal Article] Liquid chemiluminescent DNA pull-down assay to measure nuclear receptor-DNA binding in solution. (2008)
  • Author(s): Iwasaki T, Miyazaki W, Rokutanda N, Koibuchi N
  • Journal Title: BioTechniques. 45 Pages: 445-448
  • Peer Reviewed
• [Journal Article] Identification of the functional domain of thyroid hormone receptor responsible for polychlorinated biphenyl-mediated suppression of its action in vitro. (2008)
  • Author(s): Miyazaki W, Iwasaki T, Takeshita A, Tohyama C, Koibuchi N
  • Journal Title: Environ Health Perspect. 116 Pages: 1231-1236
  • Peer Reviewed
• [Journal Article] The role of thyroid hormone in developing cerebellum. (2008)
  • Author(s): Koibuchi N, Qiu CH, Miyazaki W, Iwasaki T, Shimokawa N
  • Journal Title: Cerebellum. 7 Pages: 499-500
  • NAID: 130005448782
  • Peer Reviewed
• [Journal Article] 甲状腺ホルモンを介するPCB類の作用機構 (2008)
  • Author(s): 鯉淵典之,岩崎俊晴
  • Journal Title: Biophilia. 4 Pages: 26-29
  • Peer Reviewed
• [Journal Article] Liquid chemiluminescent DNA pull-down assay to measure nuclear receptor-DNA binding in solution. (2008)
  • Author(s): Iwasaki T, et al.
  • Journal Title: Biotechniques 45 Pages: 445-448
  • Peer Reviewed
• [Journal Article] Identification of the functional domain of thyroid hormone receptor responsible for polychlorinated biphenyl-mediated suppression of its action in vitro. (2008)
  • Author(s): Miyazaki W, et al.
  • Journal Title: Environ Health Perspect. 116 Pages: 1231-1236
  • Peer Reviewed
• [Journal Article] The role of thyroid hormone in developing cerebellum. (2008)
  • Author(s): Koibuchi N, et al.
  • Journal Title: Cerebellum Pages: 499-500
  • NAID: 130005448782
• [Journal Article] ポリ塩化ビフェニルによる器官の発達、機能維持への影響〜甲状腺ホルモンを介するPCB類の作用機構 (2008)
  • Author(s): 鯉淵典之、岩崎俊晴.
  • Journal Title: Biophilia 4
• [Presentation] 4-Nonylphenolによる甲状腺ホルモン受容体を介する転写の活性化と脳発達に対する影響 (2010)
  • Author(s): 岩崎俊晴,Yu Xiong,原口瑞樹,大澤祥,Rony, Ming Xu, Kingsley Ibhazehiebo, 鯉淵典之
  • Organizer: 第13回環境ホルモン学会研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-17
• [Presentation] 臭化物化合物による甲状腺ホルモン受容体を介する正常脳発達への影響(核内ホルモン受容体による転写調節における環境化学物質の作用機構) (2010)
  • Author(s): 岩崎俊晴
  • Organizer: EXTEND2010 公開セミナー
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2010-12-15
• [Presentation] 臭化物化合物による甲状腺ホルモン受容体を介する正常脳発達への影響(核内ホルモン受容体による転写調節における環境化学物質の作用機構) (2010)
  • Author(s): 岩崎俊晴
  • Organizer: EXTEND2010 公開セミナー
  • Place of Presentation: 東京
  • Year and Date: 2010-12-15
• [Presentation] 核内ホルモン受容体による転写調節における環境化学物質の作用機構 (2010)
  • Author(s): 岩崎俊晴,下川哲昭,鯉淵典之
  • Organizer: 第23回環境ホルモン学会講演会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2010-06-14
• [Presentation] 核内ホルモン受容体による転写調節における環境化学物質の作用機構-正常脳発達への影響 (2010)
  • Author(s): 岩崎俊晴
  • Organizer: 第23回環境ホルモン学会講演会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-14
• [Presentation] Augmentation of thyroid hormone receptor-mediated transcription by 4-nonyl phenol ; its mechanism and physiological role. (2010)
  • Author(s): Iwasaki T, Osawa S, Yu X, Ibhazehiebo K, Koibuchi N
  • Organizer: 第87 回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] Low Dose Bromo-related Compounds Supress the Thyroid hormone-induced Brain Development. (2010)
  • Author(s): Iwasaki T
  • Organizer: 14 International Congress of Endocrinology(ICE)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-03-30
• [Presentation] Suppression of cerebellar Purkinje cell dendrite arborization by hexabromocyclododecane (2010)
  • Author(s): 岩崎俊晴
  • Organizer: 第83回日本内分泌学会
  • Place of Presentation: 京都
  • Year and Date: 2010-03-26
• [Presentation] 4-Nonylphenolによる甲状腺ホルモン受容体を介する転写の活性化作用 (2010)
  • Author(s): 大澤祥
  • Organizer: 第83回 日本内分泌学会
  • Place of Presentation: 京都
  • Year and Date: 2010-03-26
• [Presentation] 4-Nonylphenol による甲状腺ホルモン受容体を介する転写の活性化と脳発達に対する影響 (2010)
  • Author(s): 岩崎俊晴
  • Organizer: 第13回 環境ホルモン学会研究発表会
  • Place of Presentation: 東京
• [Presentation] Brain-derived neurotrophic factor (BDNF) ameliorates the suppression of thyroid hormone-induced granule cell neurite extension by hexabromocyclododecane (HBCD). (2010)
  • Author(s): Ibhazehiebo K
  • Organizer: 第13回 環境ホルモシ学会研究発表会
  • Place of Presentation: 東京
• [Presentation] 発達期脳におけるBrain-derived Repressive Molecule (B-ReM)の発現と機能解析 (2010)
  • Author(s): 岩崎俊晴
  • Organizer: 第53回 日本甲状腺学会
  • Place of Presentation: 長崎
• [Presentation] 甲状腺ホルモンによる脳発達に対する4-Nonylphenolの影響 (2010)
  • Author(s): 原口瑞樹
  • Organizer: 第53回日本甲状腺学会の
  • Place of Presentation: 長崎
• [Presentation] The effect of lipopolysaccharide on astrocyte plasticity. (2010)
  • Author(s): Ming Xu
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
• [Presentation] Augmentation of thyroid hormone receptor-mediated transcription by 4-nonyl phenol ; its mechanism and physiological role. (2010)
  • Author(s): Iwasaki T.
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
• [Presentation] Suppression of cerebellar Purkinje cell dendrite arborization by alpha-hexabromocyclododecane (2010)
  • Author(s): Ibhazehiebo K.
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
• [Presentation] PCBのTRシグナリングに対する影響の性差 (2009)
  • Author(s): 岩崎俊晴
  • Organizer: 第12回 環境ホルモン学会研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2009-12-07
• [Presentation] 脳発達におけるPCBのTRシグナリングに対する影響の性差 (2009)
  • Author(s): 岩崎俊晴
  • Organizer: 第52回 日本甲状腺学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-04
• [Presentation] In vivo Interaction of TR and GR with the General Coactivator SYT. (2009)
  • Author(s): Londono MB.
  • Organizer: 第52回 日本甲状腺学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-04
• [Presentation] Suppression of cerebellar Purkinje cell dendrite arborization by hexabromocyclododecane (2009)
  • Author(s): Ibhazehiebo K
  • Organizer: 第12回 環境ホルモン学会研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-04
• [Presentation] Polybrominated diphenylethers(PBDEs)suppresses Thyroid hormone receptor(TR)-mediated transcription and impairs Thyroid hormone(TH)-dependent dendrite arborization of Purkinje cells. (2009)
  • Author(s): Ibhazehiebo K
  • Organizer: 9th Asia and Oceania Thyroid Association(AOTA)
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2009-11-02
• [Presentation] Cloning and Characterization of a novel novel nuclear protein, Brain-derived Repressive Molecule(B-ReM). (2009)
  • Author(s): Iwasaki T
  • Organizer: XXXVI International Congress of Physiological Science(IUPS)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-29
• [Presentation] Early Postnatal Expression of Brain-derived Repressive Molecule(B-ReM)in developing cerebellum (2009)
  • Author(s): Yu Xiong
  • Organizer: XXXVI International Congress of Physiological Science(IUPS)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-29
• [Presentation] Low Dose Polybrominated Diphenylethers (Pbdes) Supresses Thyroid Hormone Receptor(TR-Mediated Transcription Via Dna Binding Domain. (2009)
  • Author(s): Ibhazehiebo K
  • Organizer: XXXVI International Congress of Physiological Science(IUPS)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-29
• [Presentation] The Mechanism of the Gender Difference of PCB Action on Thyroid Hormone Receptor Interaction. (2009)
  • Author(s): Ogawa A
  • Organizer: XXXVI International Congress of Physiological Science(IUPS)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-29
• [Presentation] Intracellular Traficking Of The General Coactivator SYT. (2009)
  • Author(s): Marina B.Londono
  • Organizer: XXXVI International Congress of Physiological Science(IUPS)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-29
• [Presentation] 甲状腺ホルモン受容体における環境化学物質の影響 (2009)
  • Author(s): 岩崎俊晴
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 前橋(公募シンポジウム)
  • Year and Date: 2009-04-25
• [Presentation] 甲状腺ホルモン受容体における環境化学物質の影響 (2009)
  • Author(s): 岩崎俊晴
  • Organizer: 第82回 日本内分泌学会学術総会
  • Place of Presentation: 前橋
  • Year and Date: 2009-04-25
• [Presentation] Low Dose Hexabromocyclododecane Supress Thyroid Hormone Receptor-Mediated Transcription. (2009)
  • Author(s): Ibhazehiebo K
  • Organizer: 第82回 日本内分泌学会学術総会
  • Place of Presentation: 前橋
  • Year and Date: 2009-04-25
• [Presentation] Effect of polychlorinated biphenyl (PCB) on thyroid hormone receptor (TR)-retinoic acid receptor-related orphan receptor (ROR) crosstalk and its mechanism in developing brain. (2008)
  • Author(s): Iwasaki T, et al.
  • Organizer: 6th FENS Forum of European Neuroscience
  • Place of Presentation: Geneva
• [Presentation] 新規転写抑制因子brain-derived repressive modulator (B-ReM)の同定. (2008)
  • Author(s): 岩崎俊晴
  • Organizer: 第51回日本甲状腺学会
  • Place of Presentation: 宇都宮
• [Presentation] PCBのTRシグナリングに対する影響の性差. (2008)
  • Author(s): 岩崎俊晴
  • Organizer: 第11回環境ホルモン学会研究発表会
  • Place of Presentation: 東京
• [Presentation] 新たな機序によるcoactivator mopdulator(CoAM)による核内ホルモン受容体を介する転写の枷制. (2008)
  • Author(s): 岩崎俊晴
  • Organizer: 第81回日本内分泌学会学術総会.
  • Place of Presentation: 青森
• [Presentation] 化学物質の毒性評価のための新たなアッセイ法〜Liquid Chemilumines cent DNA-pull Down Assay. (2008)
  • Author(s): 岩崎俊晴
  • Organizer: イノベーションジャパン.
  • Place of Presentation: 東京
• [Book] 生物の事典(ヒトの最新医療) (2010)
  • Author(s): 岩崎俊晴,鯉淵典之
  • Publisher: 朝倉書店
• [Book] 生物学辞典(石川 統, 他編集) (2010)
  • Author(s): 岩崎俊晴, 他
  • Publisher: 東京化学同人
• [Book] 生物学辞典 (2010)
  • Author(s): 石川統, 他編集、岩崎俊晴, 他
  • Publisher: 東京化学同人
• [Book] 生物の事典 (2010)
  • Author(s): 石原勝敏, 他編集、岩崎俊晴, 他
  • Total Pages: 542
  • Publisher: 朝倉書店
• [Book] Principle and Practice,内分泌・代謝 (2010)
  • Author(s): 寺内康夫, 他編集、岩崎俊晴, 他
  • Total Pages: 344
  • Publisher: 分光堂
• [Book] 生物の事典.「人類の最新医療.現代人を取り巻く疾病」 (2010)
  • Author(s): 岩崎俊晴
  • Publisher: 朝倉書店(In press)
• [Book] 現代人を取り巻く疾病、生物の事典 (2009)
  • Author(s): 岩崎俊晴、鯉淵典之.
  • Publisher: 朝倉書店(in press)
• [Book] Principle and Practice, 内分泌・代謝(寺内康夫, 他編集)
  • Author(s): 岩崎俊晴,鯉淵典之
  • Publisher: 文光堂
• [Book] 生物学大事典
  • Author(s): 岩崎俊晴
  • Publisher: 東京化学同人(In press)