Immunofluorescence imaging analysis for intra- and inter-cellular signal transduction mechanisms in chemical carcinogenesis models

• Project/Area Number: 20510071
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East
• Principal Investigator: IMAI Toshio National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East, 研究所, 支援施設長 (20342884)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 化学発がん / ラット / シグナル伝達 / イメージング解析 / 多重蛍光染色 / 免疫組織化学 / イメージング / 細胞内局在 / 乳腺 / シグナル伝達物質 / 定量 / 大腸

Research Abstract

Carcinoma tissues obtained from chemically induced rat colorectal and mammary carcinogenesis models were analyzed by a multiple fluorescence method. Frozen tissue sections were stained for beta-catenin and bcl-1 in a colorectal model and estrogen receptor (ER) alpha and leptin receptor (ObR) in a mammary model. Analyses for their co-localization and luminance were performed, and the results might indicate existence of negative feedback systems between beta-catenin and bcl-1 and strong association between ER alpha and ObR in each model.

Research Products

• [Journal Article] Novel immunohistochemical marker, integrin αVβ_3, for BOP-induced early lesions in hamster pancreatic ductal carcinogenesis. (2011)
  • Author(s): Tsukasa Kitahashi, Mitsuyoshi Yoshimoto, Toshio Imai
  • Journal Title: Oncology Letters 2 Pages: 229-234
• [Journal Article] Novel immunohistochemical marker, integrin α_vβ_3, for BOP-induced early lesions in hamster pancreatic ductal carcinogenesis. (2011)
  • Author(s): Tsukasa Kitahashi
  • Journal Title: Oncology Letters Volume: VOL.2 Pages: 229-234
  • Peer Reviewed
• [Presentation] 卵巣摘除ラットにおけるDMBA誘発乳腺発がんに対する血中アディポカインの影響 (2011)
  • Author(s): 今井俊夫、亘理尭、菅野和夫、早川拓也、北橋宗
  • Organizer: 第27回日本毒性病理学会
  • Place of Presentation: 大阪国際交流センター(大阪市)
  • Year and Date: 2011-01-27
• [Presentation] 卵巣摘除ラットにおけるDMBA誘発性乳腺発がんに対する高レプチン血症の影響 (2010)
  • Author(s): 今井俊夫、北橋宗、早川拓也、高橋真美
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-09-22
• [Presentation] OP誘発性ハムスター膵管腫瘍に対する新たな免疫組織化学的マーカーとしてのインテグリンαVβ3の有用性 (2009)
  • Author(s): 今井俊夫、北橋宗、吉本光喜、中釜斉
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-10-01
• [Remarks]
  • URL: http://www.ncc.go.jp/jp/nccri/monrep/index.html