Immunofluorescence imaging analysis for intra- and inter-cellular signal transduction mechanisms in chemical carcinogenesis models
Project/Area Number |
20510071
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Risk sciences of radiation/Chemicals
|
Research Institution | National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East |
Principal Investigator |
IMAI Toshio National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East, 研究所, 支援施設長 (20342884)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | 化学発がん / ラット / シグナル伝達 / イメージング解析 / 多重蛍光染色 / 免疫組織化学 / イメージング / 細胞内局在 / 乳腺 / シグナル伝達物質 / 定量 / 大腸 |
Research Abstract |
Carcinoma tissues obtained from chemically induced rat colorectal and mammary carcinogenesis models were analyzed by a multiple fluorescence method. Frozen tissue sections were stained for beta-catenin and bcl-1 in a colorectal model and estrogen receptor (ER) alpha and leptin receptor (ObR) in a mammary model. Analyses for their co-localization and luminance were performed, and the results might indicate existence of negative feedback systems between beta-catenin and bcl-1 and strong association between ER alpha and ObR in each model.
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Report
(4 results)
Research Products
(6 results)