Molecular breeding of transgenic tree for phytoremediation of aromatic compounds

• Project/Area Number: 20510076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Mie University
• Principal Investigator: KIMURA Tetsuya Mie University, 大学院・生物資源学研究科, 准教授 (00281080)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 白色不朽菌 / マンガンペルオキシダーゼ / ヒラタケ / シロイヌナズナ / ビスフェノールA / ポプラ / 塩化安息香酸 / トキイロヒラタケ / ラッカーゼ / 組織特異的プロモーター / 白色腐朽菌 / プロモーター / レマゾールブリリアントブルー / 多環芳香族

Research Abstract

Many white-rod fungi produce many lignin peroxidases. Among them, Mnp2 of Pleurotus ostreatus was reported to degrade recalcitrant polyaromatic compounds because of its strong peroxidase activity. We introduced a fusion gene encoding poplar cellulase signal sequence and mnp2 mature region under the control of leaf specific or root specific promoter into Arabidopsis thaliana. Several transgenic plants showed the ability to degrade BisphenolA. This suggests the possibility of molecular breeding of transgenic tree which can express the mnp2 gene. We also introduced a chlorocatechol dioxigenase gene of a soil bacterium Ralstonia eutropha NH9 under the control of CaMV35S promoter into poplar plant (hybrid aspen) and this gene was successfully expressed in the transgenic poplar plant.

Research Products

• [Journal Article] Stable production of thermotolerant xylanaseB of Clostridium stercorarium in transgenic tobacco and rice. (2010)
  • Author(s): T.Kimura, T.Mizutani, JL.Sun, T.Kawazu, S.Karita, M.Sakka, Y.Kobayashi, K.Ohmiya, K.Sakka
  • Journal Title: Biosci.Biotechnol.Biochem. 74(3) Pages: 954-960
  • Peer Reviewed
• [Journal Article] Gateway対応バイナリーベクターの開発と植物分子生物学への応用 (2010)
  • Author(s): 木村哲哉、中川強
  • Journal Title: 三重大学大学院生物資源学研究科紀要 36 Pages: 1-12
  • NAID: 120005855972
  • Peer Reviewed
• [Journal Article] Stable production of thermotolerant xylanaseB of Clostridium stercorarium in transgenic tobacco and rice (2010)
  • Author(s): T.Kimura, T.Mizutani, et al.
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: 74 Pages: 954-960
  • Peer Reviewed
• [Journal Article] Development of Gateway binary vectors, R4L1p GWBs, for promoter analysis in higher plants. (2009)
  • Author(s): S.Nakamura, A, Nakao, M. Kawamukai, T.Kimura, S.Ishiguro, T.Nakagawa
  • Journal Title: Bisci.Biotechnol.Biochem. 73(11) Pages: 2556-2559
  • NAID: 10027548374
  • Peer Reviewed
• [Journal Article] Stable expression of the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 in hybrid poplar cells (2009)
  • Author(s): Y.Ohmiya, T.Ono, T.Taniguchi, N.Itahana, N.Ogawa, K.Miyashita, K.Ohmiya, K.Sakka, T.Kimura
  • Journal Title: Biosci.Biotechnol.Biochem. 73(6) Pages: 1425-1428
  • NAID: 10027542830
  • Peer Reviewed
• [Journal Article] Gateway vectors for plant transformation (2009)
  • Author(s): T.Nakagawa, S.Ishiguro, T.Kimura
  • Journal Title: Plant Biotechnol.J. 26 Pages: 275-284
  • NAID: 10024853760
  • Peer Reviewed
• [Journal Article] Stable expression of the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 in hybrid poplar cells (2009)
  • Author(s): Ohmiya, et al.
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 1425-1428
  • Peer Reviewed
• [Journal Article] Development of gateway binary vectors, R4L1pGWBs, for promoter analysis in higher plants (2009)
  • Author(s): Nakamura, et al.
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 2556-2559
  • Peer Reviewed
• [Journal Article] Gateway vectors for plant transformation (2009)
  • Author(s): Nakagawa, et al
  • Journal Title: Plant Biotechnol 26 Pages: 275-284
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 井上國世, ら(共著)
  • Journal Title: 産業用酵素の応用技術と最新動向(シーエムシー出版) Pages: 329-335
• [Journal Article] Stable expression of a chlorocatechol dioxygenase gene in poplar (2009)
  • Author(s): Y. Ohraiya, T. Ono, T. Ta niguchi, et al
  • Journal Title: Biosci. Biotechnol. Biochem. (in press)
  • Peer Reviewed
• [Presentation] 白色不朽菌リグニン分解酵素遺伝子の導入とその解析 (2010)
  • Author(s): 領木智哉、木村哲哉, 他9名
  • Organizer: 日本分子生物学会第33回大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-08
• [Presentation] 白色不朽菌リグニン分解酵素遺伝子の植物への導入とその解析 (2010)
  • Author(s): R.Tomoya, et al.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-08
• [Presentation] リサイクルクローニングシステム-植物形質転換における遺伝子多重コンストラクト構築の新技術- (2008)
  • Author(s): 木村哲哉, 中尾彰秀, 中村真也, 河津哲, 中川強
  • Organizer: 日本分子生物学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] Stabele expression of the mnp3 gene of a white-rot fungus Pleurotus ostreatus in Arabidopsis thaliana by Gateway Binary Vector (2008)
  • Author(s): 木村哲哉
  • Organizer: Mie Bioforum
  • Place of Presentation: Shima-Isobe Japan
  • Year and Date: 2008-09-01
• [Presentation] Detoxification of chlorocatechol by transgenic plant expressing the chlorocatechol dioxygenase and chloromuconate cycloisomearase genes of Ralstonia eutropha NH9 (2008)
  • Author(s): 木村哲哉
  • Organizer: Mie Bioforum
  • Place of Presentation: Shima-Isobe Japan
  • Year and Date: 2008-09-01
• [Book] 産業酵素の応用技術と動向、井上國世監修有機塩素化合物(環境汚染物質の分解)(ISBN978-4-7813-0108-2C3045) (2009)
  • Author(s): 木村哲哉、粟冠和郎
  • Publisher: エムシー出版
• [Remarks] ホームページ等
  • URL: http://www.bio.mie-u.ac.jp/junkan/kohgaku/lab4/
• [Remarks]
  • URL: http://www.bio.mie-u.ac.jp/junkan/kohgaku/lab4/plantbio/plantbio.html