Molecular breeding of transgenic tree for phytoremediation of aromatic compounds
Project/Area Number |
20510076
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Environmental technology/Environmental materials
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Research Institution | Mie University |
Principal Investigator |
KIMURA Tetsuya Mie University, 大学院・生物資源学研究科, 准教授 (00281080)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 白色不朽菌 / マンガンペルオキシダーゼ / ヒラタケ / シロイヌナズナ / ビスフェノールA / ポプラ / 塩化安息香酸 / トキイロヒラタケ / ラッカーゼ / 組織特異的プロモーター / 白色腐朽菌 / プロモーター / レマゾールブリリアントブルー / 多環芳香族 |
Research Abstract |
Many white-rod fungi produce many lignin peroxidases. Among them, Mnp2 of Pleurotus ostreatus was reported to degrade recalcitrant polyaromatic compounds because of its strong peroxidase activity. We introduced a fusion gene encoding poplar cellulase signal sequence and mnp2 mature region under the control of leaf specific or root specific promoter into Arabidopsis thaliana. Several transgenic plants showed the ability to degrade BisphenolA. This suggests the possibility of molecular breeding of transgenic tree which can express the mnp2 gene. We also introduced a chlorocatechol dioxigenase gene of a soil bacterium Ralstonia eutropha NH9 under the control of CaMV35S promoter into poplar plant (hybrid aspen) and this gene was successfully expressed in the transgenic poplar plant.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article]2009
Author(s)
井上國世, ら(共著)
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Journal Title
産業用酵素の応用技術と最新動向(シーエムシー出版)
Pages: 329-335
Related Report
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