| Project/Area Number |
20510111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
ISHIKAWA Mitsuru National Institute of Advanced Industrial Science and Technology, 健康工学研究部門, 研究グループ長 (70356434)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Tamitake 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (00351742)
ABE Hiroko 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究員 (40363220)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ナノ粒子・ナノチューブ / バイオイメージング / 表面増強ラマン散乱 / 出芽酵母 / 細胞周期 / 細胞分裂 / マンノタンパク質 / 暗視野照明 / 弾性散乱光 / 細胞表面 / 表面増強ハイパーラマン散乱 / 大腸菌 / 糖化ヘモグロビン / 局在表面プラズマ共鳴 / 酵母 |
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| Research Abstract |
The purpose of the current project is in-situ detection of protein molecules on the surface of a single cell in a highly-sensitive manner. Key proteins on the surface will appear and disappear within several minutes in a cell cycle. Thus, measurement much faster than that by conventional Raman spectroscopy is highly required. We select surface-enhanced Raman spectroscopy (SERS) to meet the requirement. We observed a cell cycle of a single yeast cell as a model system with the help of SERS from a protein in a non-invasive manner. Furthermore, the protein is successfully identified by SERS spectroscopy and inhibition assays for the protein.
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