Development of the technique to control single molecule fluorescence emitters and its measurement system

• Project/Area Number: 20510112
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: YAMADA Toshiki National Institute of Information and Communications Technology, 未来ICT研究センターナノICTグループ, 主任研究員 (10359101)
• Research Collaborator: KAJI Takahiro 独立行政法人情報通信研究機構, 未来ICT研究センターナノICTグループ, 専攻研究員 (40573134)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 時間相関単一光子計数システム / イオン性液体 / 単一蛍光体 / 液浸対物レンズ / 単一分子分光 / 単一光子光源 / 時間相関単一光子計数 / 高真空 / 高純度窒素 / 電場印加発光制御 / フォトニック結晶

Research Abstract

We performed the evaluation and control of fluorescence characteristics of a single fluorescence emitter under various ambient- and electromagnetic-controlled conditions. We also developed a time-correlated single photon counting system with modified photomultiplier tubes as photon detectors as well as a new microscope light collection unit having an immersion objective with high numerical aperture and ionic liquid used as a refractive index matching medium. The results achieved in the present study provide us with a wide range of applications for nano-bio sensing and fabrication of optical devices.

Research Products

• [Journal Article] Fabrication of two-dimensional Ta205 photonic crystal slabs with ultra-low background emission toward highly sensitive fluorescence spectroscopy (2011)
  • Author(s): Takahiro Kaji
  • Journal Title: Optics Express Volume: 19 Pages: 1422-1428
  • Peer Reviewed
• [Journal Article] Optimization of microscope unit for studying fluorescence emitters undue high-vacuum and ambient gas conditions : Optical properties for various ionic liquids as a refractive index matching medium (2011)
  • Author(s): Toshiki Yamada
  • Journal Title: Physics Procedia Volume: (To be published)
  • Peer Reviewed
• [Journal Article] Fabrication of two-dimensional Ta2O5 photonic crystal slabs with ultra-low background emission toward highly sensitive fluorescence spectroscopy (2010)
  • Author(s): Takahiro Kaji, Toshiki Yamada, Rieko Ueda, Xingsheng Xu, Akira Otomo
  • Journal Title: Optics Express 19 Pages: 1422-1428
  • Peer Reviewed
• [Journal Article] Modification of two-photon excited fluorescence from quantum dots in SiN photonic crystals (2010)
  • Author(s): Xingsheng Xu, Toshiki Yamada, Shiyoshi Yokoyama
  • Journal Title: Optics Letters 35 Pages: 309-311
  • Peer Reviewed
• [Journal Article] Antibunching and blinking from a single colloidal CdSe quantum dot (2010)
  • Author(s): Xingsheng Xu, Shuai Chen, Toshiki Yamada
  • Journal Title: Science China 53 Pages: 1619-1625
  • Peer Reviewed
• [Journal Article] Time correlated single photon counting system with a modified objective unit for studying fluorescence emitters under high-vacuum and ambient gas conditions (2010)
  • Author(s): Toshiki Yamada, Akira Otomo
  • Journal Title: Japanese Journal of Applied Physics 49
  • Peer Reviewed
• [Journal Article] Antibunching and blinking from a single colloidal CdSe quantum dot (2010)
  • Author(s): Xu Xingsheng
  • Journal Title: Science China Volume: 53 Pages: 1619-1625
  • Peer Reviewed
• [Journal Article] Modification of two-photon excited fluorescence from quantum dots on SiN photonic crystals (2010)
  • Author(s): Xu Xingsheng
  • Journal Title: Optics Letters Volume: 35 Pages: 309-311
  • Peer Reviewed
• [Journal Article] Time-correlated single-photon counting system with a modified objective unit for studying fluorescence emitters under high-vacuum and ambient gas conditions (2010)
  • Author(s): Toshiki Yamada
  • Journal Title: Japanese Journal of Applied Physics 49
  • Peer Reviewed
• [Journal Article] 蛍光体研究のための時間相関単一光子計数系及び高真空及び周囲ガス環境下における高効率集光系の開発-イオン性液体と液浸対物レンズの使用- (2009)
  • Author(s): 山田俊樹
  • Journal Title: 情報通信学会技術研究報 Vol.109(No.251) Pages: 35-40
• [Journal Article] Excitation intensity dependence of power-law statistics in nanocrystal quantum dots (2009)
  • Author(s): Kenichi Goushi, Toshiki Yamada, Akira Otomo
  • Journal Title: Journal of Physical Chemstry C 113 Pages: 20161-20168
  • Peer Reviewed
• [Journal Article] Two-photon excited fluorescence from CdSe quantum dots on SiN photonic crystals (2009)
  • Author(s): Xingsheng Xu, Toshiki Yamada, Rieko Ueda, Akira Otomo,
  • Journal Title: Applied Physics Letter 95
  • Peer Reviewed
• [Journal Article] Corrlation between antibunching and blinking of photoluminescence from a single CdSe quantum dot (2009)
  • Author(s): Xingsheng Xu, Toshiki Yamada, Shiyoshi Yokoyama
  • Journal Title: The European Physics Journal D 55 Pages: 691-697
  • Peer Reviewed
• [Journal Article] Time-correlated single photon counting system and light-collection system for studying fluorescence emitters under high-vacuum condition : Use of immersion objective and ionic liquid (2009)
  • Author(s): Toshiki Yamada, Akira Otomo
  • Journal Title: Thin Solid Films 518 Pages: 432-4369
  • Peer Reviewed
• [Journal Article] Time-correlated single photon counting system and light-collection system for studying fluorescence emitters under high-vacuum condition : Use of immersion objective and ionic liquid (2009)
  • Author(s): Toshiki Yamada
  • Journal Title: Thin Solid Films 518 Pages: 432-436
  • Peer Reviewed
• [Journal Article] Corrlation between antibunching and blinking of photoluminescence from a single CdSe quantum dot (2009)
  • Author(s): Xingsheng Xu
  • Journal Title: The European Physics Journal D 55 Pages: 691-697
  • Peer Reviewed
• [Journal Article] Two-photon excited fluorescence from CdSe quantum dots on SiN photonic crystals (2009)
  • Author(s): Xingsheng Xu
  • Journal Title: Applied Physics Letters 95
  • Peer Reviewed
• [Journal Article] Excitation intensity dependence of power-law statistics in nanocrystal quantum dots (2009)
  • Author(s): Kenichi Goushi
  • Journal Title: Journal of Physical Chemstry C 113 Pages: 20161-20168
  • Peer Reviewed
• [Journal Article] 蛍光体研究のための時間相関単一光子計数系及び高真空及び周囲ガス環境下における高効率集光系の開発 -イオン性液体と液浸対物レンズの使用- (2009)
  • Author(s): Toshiki Yamada
  • Journal Title: 情報通信学会技術研究報告 109(No.251) Pages: 35-40
• [Journal Article] Photostability of single-photon emission from a single quantum dot in the 650-nm wavelength band at room temperature (2009)
  • Author(s): Xingsheng Xu
  • Journal Title: Applied Physics B 94 Pages: 577-583
  • Peer Reviewed
• [Journal Article] Time-correlated single photon counting system and light-collection system for studying fluorescence emitters under high-vacuum conditions : Use of immersion objective and ionic liquid (2009)
  • Author(s): Toshiki Yamada
  • Journal Title: Thin Solid Films (印刷中)
  • Peer Reviewed
• [Journal Article] Single-photon source in strong photon-atom interaction regime in quasiperiodic photonic crystals (2008)
  • Author(s): Xingsheng Xu, Hongda Chen, Toshiki Yamada, Akira Otomo
  • Journal Title: Europhysics Letters 84
  • Peer Reviewed
• [Journal Article] Photostability of single-photon emission from a single quantum dot in the 650-nm wavelength band at room temperature (2008)
  • Author(s): Xignsheng Xu, Toshiki Yamada, Akira Otomo
  • Journal Title: Applied Physics B 93 Pages: 539-543
  • Peer Reviewed
• [Journal Article] Time correlated single photon counting and optical measurement systems for studying single fluorescent emitters under high vacuum conditions (2008)
  • Author(s): Toshiki Yamada, Kenichi Goushi, Xingsheng Xu, Akira Otomo
  • Journal Title: Thin Solid Films 517 Pages: 1507-1511
  • Peer Reviewed
• [Journal Article] Time-correlated single photon counting and measurement systems for single fluorescent emitters under high vacuum conditions (2008)
  • Author(s): Toshiki Yamada
  • Journal Title: Thin Solid Films 517 Pages: 1507-1511
  • Peer Reviewed
• [Journal Article] Single-photon source in strong photon-atom interaction regime in quasiperiodic photonic crystals (2008)
  • Author(s): Xingsheng Xu
  • Journal Title: Europhysics Letters 84
  • Peer Reviewed
• [Journal Article] Optimization of microscope unit for studying fluore scence emitters under high-vacuum and ambient gas conditions : Optical properties for various ionic liquids as a refractive index matching medium
  • Author(s): Toshiki Yamada, Akira Otomo
  • Journal Title: to be published in Physics Procedia.
  • Peer Reviewed
• [Presentation] 高感度蛍光顕微鏡法実現に向けた2次元Ta2O5フォトニック結晶の作製 (2011)
  • Author(s): 梶貴博
  • Organizer: 2011年春季第58回応用物理学関連連合講演
• [Presentation] 超低バックグラウンド発光の2次元フォトニック結晶を用いたペリレンビスイミドの蛍光増強 (2011)
  • Author(s): 梶貴博
  • Organizer: 日本化学会第91春季年会(2011)
• [Presentation] Enhancement of dye fluorescence using photonic crystal slabs with ultra-low background emission (2011)
  • Author(s): 梶貴博
  • Organizer: 6^th International Conference on Molecular Electronics and Bioelectronics
• [Presentation] Enhancement of dye fluorescence using photonic crystal slabs with ultra-low background emission (2011)
  • Author(s): Takahiro Kaji
  • Organizer: 6^<th> International Conference on Molecular Electronics and Bioelectronics
  • Place of Presentation: 予稿集による発表扱い
• [Presentation] 高感度蛍光顕微鏡法実現に向けた2次元Ta2O5フォトニック結晶の作製 (2011)
  • Author(s): 梶貴博
  • Organizer: 2011年春季第58回応用物理学関連連合講演会
  • Place of Presentation: 予稿集による発表扱い
• [Presentation] Optimization of microscope unit for studying fluorescence emitters undue high-vacuum and ambient gas conditions (2010)
  • Author(s): 山田俊樹
  • Organizer: 9th International Conference on Nano Molecular Electronics
  • Place of Presentation: 神戸国際会議場、兵庫県
  • Year and Date: 2010-12-15
• [Presentation] Optimization of microscope unit for studying fluorescence emitters undue high-vacuum and ambient gas conditions (2010)
  • Author(s): Toshiki Yamada
  • Organizer: 9^<th> International Conference on Nano-Molecular Electronics
  • Place of Presentation: 神戸国際会議場、兵庫県
  • Year and Date: 2010-12-15
• [Presentation] Fabrication of low ackground two-dimensional Ta2O5 photonic rystals for observing fluorescence nhancement of organic dyes (2010)
  • Author(s): 梶貴博
  • Organizer: 9th International Conference on Nano Molecular Electronics
  • Place of Presentation: 神戸国際会議場、兵庫県
  • Year and Date: 2010-12-14
• [Presentation] Fabrication of low background two-dimensional Ta2O5 photonic crystals for observing fluorescence enhancement of organic dyes (2010)
  • Author(s): Takahiro Kaji
  • Organizer: 9^<th> International Conference on Nano-Molecular Electronics
  • Place of Presentation: 神戸国際会議場、兵庫県
  • Year and Date: 2010-12-14
• [Presentation] 2次元フォトニック結晶基板上に吸着した色素分子の発光挙動 (2010)
  • Author(s): 梶貴博
  • Organizer: 2010年光化学討論会
  • Place of Presentation: 千葉大学,千葉県
  • Year and Date: 2010-09-08
• [Presentation] 2次元フォトニック結晶基板上に吸着した色素分子の発光挙動 (2010)
  • Author(s): 梶貴博
  • Organizer: 2010年光化学討論会
  • Place of Presentation: 千葉大学、千葉県
  • Year and Date: 2010-09-08
• [Presentation] Optimization of microscope objective unit for studying fluorescence emitters under high-vacuum and ambient gas conditions and its applications (2010)
  • Author(s): 山田俊樹
  • Organizer: 6th International Symposium on Organic Molecular Electronics
  • Place of Presentation: 千葉大学,千葉県
  • Year and Date: 2010-06-11
• [Presentation] Optimization of microscope objective unit for studying fluorescence emitters under high-vacuum and ambient gas conditions and its applications (2010)
  • Author(s): Toshiki Yamada
  • Organizer: 6^<th> International Symposium on Organic Molecular Electronics
  • Place of Presentation: 千葉大学、千葉県
  • Year and Date: 2010-06-11
• [Presentation] イオン性液体と液浸対物レンズを用いた高真空及び周囲ガス環境下の高効率集光系の開発と応用 (2010)
  • Author(s): 山田俊樹
  • Organizer: 第57回応用物理学関係連合講演会
  • Place of Presentation: 東海大学、神奈川県
  • Year and Date: 2010-03-19
• [Presentation] 蛍光体研究のための時間相関単一光子計数系及び高真空及び周囲ガス環境下における高効率集光系の開発-イオン性液体と液侵対物レンズの使用- (2009)
  • Author(s): 山田俊樹
  • Organizer: 電子情報通信学会有機エレクトロニクス研究会
  • Place of Presentation: 中央電気倶楽部,大阪府
  • Year and Date: 2009-10-23
• [Presentation] 蛍光体研究のための時間相関単一光子計数系及び高真空及び周囲ガス環境下における高効率集光系の開発 -イオン性液体と液浸対物レンズの使用- (2009)
  • Author(s): 山田俊樹
  • Organizer: 電子情報通信学会有機エレクトロニクス研究会
  • Place of Presentation: 中央電気倶楽部、大阪府
  • Year and Date: 2009-10-23
• [Presentation] 時間相関単一光子計数系と 高真空中高効率集光系-イオン性液体と液浸対物レンズの使用- (2009)
  • Author(s): 山田俊樹
  • Organizer: 第56回応用物理学関係連合講演会
  • Place of Presentation: 筑波大学,茨城県
  • Year and Date: 2009-03-30
• [Presentation] 時間相関単一光子計数系と高真空中高効率集光系 -イオン性液体と液浸対物レンズの使用- (2009)
  • Author(s): 山田俊樹
  • Organizer: 第56回応用物理学関係連合講演会
  • Place of Presentation: 筑波大学
  • Year and Date: 2009-03-30
• [Presentation] 高真空中時間相関単一光子 検出系と単一発光体蛍光挙動解析 (2009)
  • Author(s): 山田俊樹
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 日本大学理工学部船橋キャンパス,千葉県
  • Year and Date: 2009-03-28
• [Presentation] 時間相関単一光子検出系と単一発光体蛍光挙動解析 (2009)
  • Author(s): 山田俊樹
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2009-03-28
• [Presentation] Time-correlated single photon counting system and modified objective unit for studying fluorescence emitters under high vacuum condition (2009)
  • Author(s): 山田俊樹
  • Organizer: Fifth International Conference on Molecular Electronics and Bioelectronics
  • Place of Presentation: 宮崎国際会議場,宮崎県
  • Year and Date: 2009-03-17
• [Presentation] Time-correlated single photon counting system and modified objective unit for studying fluorescence emitters under high vacuum conditions (2009)
  • Author(s): Toshiki Yamada
  • Organizer: Fifth International Conference Molecular Electronics and Bioelectronics
  • Place of Presentation: 宮崎国際会議場
  • Year and Date: 2009-03-17
• [Presentation] Fluorescence characterization of single emitters and development of modified microscope unit in high vacuum in conjunction with time-correlated single photon counting system (2008)
  • Author(s): 山田俊樹
  • Organizer: 8th International Conference on Nano-Molecular Electronics
  • Place of Presentation: 神戸ポートピアホテル,兵庫県
  • Year and Date: 2008-12-16
• [Presentation] Fluorescence characterization of single emitters and development of modified microscope units under high-vacuum conditions in conjunction with time-correlated single photon counting systems (2008)
  • Author(s): Toshiki Yamada
  • Organizer: 8^<th> International Conference on Nano-Molecular Electronics
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-16
• [Presentation] Time-correlated single photon counting and optical measurement systems for studying single fluorescence emitters in high vacuum conditions (2008)
  • Author(s): 山田俊樹
  • Organizer: 2nd International Symposium on the Manipulation of Advanced Smart Materials
  • Place of Presentation: 淡路夢舞台国際会議場,兵庫県
  • Year and Date: 2008-05-29
• [Presentation] Time-correlated single photon counting and optical measurement systems for studying single fluorescence emitters in high vacuum (2008)
  • Author(s): Toshiki Yamada
  • Organizer: 2^<nd> International Symposium on the Manipulation of Advanced Smart Materials
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2008-05-29
• [Patent(Industrial Property Rights)] フォトニック結晶 (2011)
  • Inventor(s): 梶貴博, 山田俊樹, 大友明
  • Industrial Property Rights Holder: (独)情報通信研究機構
  • Filing Date: 2011-03-03
• [Patent(Industrial Property Rights)] フォトニック結晶 (2011)
  • Inventor(s): 梶貴博, 山田俊樹, 大友明
  • Industrial Property Rights Holder: 情報通信研究機構
  • Industrial Property Number: 2011-046586
  • Filing Date: 2011-03-03
• [Patent(Industrial Property Rights)] 試料観測方法、光学顕微鏡及び蛍光相関分析装置 (2008)
  • Inventor(s): 山田俊樹
  • Industrial Property Rights Holder: (独)情報通信研究機構
  • Filing Date: 2008-12-12
• [Patent(Industrial Property Rights)] 試料観測方法、光学顕微鏡及び蛍光相関分析装置 (2008)
  • Inventor(s): 山田俊樹
  • Industrial Property Rights Holder: (独)情報通信研究機構
  • Industrial Property Number: 2008-316489
  • Filing Date: 2008-12-12