development of a biosensor for heavy metal ions based on mercury-responsive transcription factor(MerR) and local plasmon enhanced excitation
| Project/Area Number |
20510117
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
ISHIDA Akito 京都府立大学, 大学院・生命環境科学研究科, 准教授 (20184525)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 局在プラズモン / マイクロ流体デバイス / プラズモン増強蛍光 / 重金属耐性遺伝子 / 水銀耐性大腸菌 / スプリット蛍光蛋白 / ナノコーンアレイ / 蛍光蛋白発現 / merR / 花形金銀コロイド / 重金属イオン耐性遺伝子 / 重金属イオン耐性大腸菌 / 蛍光蛋白発現遺伝子 / 金コロイド / ギャップモード形成 / 親水性アミノシラン |
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| Research Abstract |
The aim of this study was to develop a protein-based heavy metal sensor for field analysis. Gene constructs consisting of mercury-responsive transcription factor(MerR) and split yellow fluorescent protein or luciferase were prepared. The resulting E-coli and the isolated proteins showed properties of mercury ion sensor, while the sensitivities were not sufficient for practical use. Plasmonic materials such as gold and silver colloids have been employed to enhance the excitation and emission efficiency. Novel protocol for effective preparation and immobilization of flower-like gold-silver hybrid colloids on a glass substrate was established and the plasmonic substrate showed remarkable fluorescence enhancement by visible light excitation. The resulting E coli or isolated protein was immobilized on the plasmonic substrate using a PDMS microfluidics system and tested for a mercury sensor.
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Report
(4 results)
Research Products
(19 results)
