| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
The toxic valuation method of the fire gas by the human alveolus basal epithelium cells A549 was established as an alternative method of animal experiments, such as rat and mouse. The toxic assessment system of fire gas consists of fire gas collection equipment, exposure equipment, and toxic evaluation equipment. In the ABS resin experiment, the number of initial cells ; 3x10^5 cells/ml increased to 3x10^6 cells/ml after three-day fire gas exposure, and survival ratio was 95% under 5%-CO_2environment. It increased to 1x10^6 cells/ml and survival ratio was 90% under atmospheric environment. It decreased to 5x10^4 cells/ml and survival ratio became 20% under fire gas environment.
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