| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
Plant nuclear genes encoding tRNA^<Tyr> and tRNA^<Met-e> are interrupted by introns. Splicing of precursor tRNAs is occurred in nucleus. However, tRNA ligase involved in ligation of 5'- and 3'- half of tRNA is localized in chloroplast as well. In order to evaluate the possibility that tRNA ligase plays as a role of repair on chloroplast tRNAs, dominant-negative tobacco mutant where a mutated tRNA ligase gene with amino acid substitution in the ligase domain is over-expressed. Wild-type and mutant plants were both subjected to oxidative stress and total tRNAs were extracted from these plants. Ratios of intact to cleaved tRNAs from cytoplasm and chloroplast were determined with northern analysis using specific tRNA probes. Results showed that several of cytosolic tRNAs are cleaved with the condition of 10mM H_2O_2, but there no significant difference of ratios of intact to degrading chloroplast tRNAs between wild-type and mutated plants.
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