• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the unculturable microbial populations in human gut microbiota

Research Project

Project/Area Number 20510187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 基礎ゲノム科学
Research InstitutionThe University of Tokushima

Principal Investigator

KUWAHARA Tomomi  The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 准教授 (60263810)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Haruyuki  徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (80294669)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords腸内菌叢 / 難培養菌 / ゲノム / Segmented filamentous bacteria / シークエンス / 代謝経路 / 腸管免疫 / Th17 / ヒト腸内菌 / 難培養性細菌 / フラジェリン / 菌叢解析 / ゲルマイクロドロップ法 / 16S rDNA / セルソーター / 多様性
Research Abstract

Human harbors the numerous microbes at environmentally exposed anatomical sites. These microbes constitute the unique ecosystem called resident microflora. Gut microflora is the largest microbial ecosystem in human, which contains over 100 trillions of microbes of nearly 1,000 species. Gut microflora provides beneficial effects to the host through digestion of nutrients, synthesis of essential vitamins, and colonization resistance to enteric pathogens. However, the molecular mechanism of host-microbe symbiosis in the gut is largely unknown due to the fact that the majority of gut microflora is unculturable bacteria. It is important to disclose the biology of the unculturable populations to address the molecular interaction between the host and intestinal microbes. Herein, we determined the whole genome sequence of segmented filamentous bacteria that are representative gut unculturable microbes.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (28 results)

All 2011 2010 2009 2008

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (6 results) Book (2 results)

  • [Journal Article]2011

    • Author(s)
      Atarashi T, Tanoue T, Shima T, Imaoka A, Kuwahara T,, Momose Y, Cheng G, Yamasaki S, Saito T, Ohba Y, Taniguchi T, Takeda K, Hori S, Ivanov II, Umesaki Y, Itoh K, Honda K.
    • Journal Title

      Science 331

      Pages: 337-341

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Blockade of Th1 chemokine receptors ameliorates pulmonary granulomatosis in mice.2011

    • Author(s)
      Kishi J, Nishioka Y, Kuwahara T,, Kakiuchi S, Azuma M, Aono Y, Makino H, Kinoshita K, Kishi M, Batmunkh R, Uehara H, Izumi K, Sone S.
    • Journal Title

      Eur Respir J in press.

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of colonic regulatory T cell by indigenous Clostridium species.2011

    • Author(s)
      Atarashi K, et al.
    • Journal Title

      Science

      Volume: 331 Pages: 337-341

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Utilization of titanium oxide-like compound as an inorganic phosphate adsorbent for the control of serum phosphate level in chronic renal failure.2010

    • Author(s)
      Tamagawa K, Nakayama-Imaohji H, Wakimoto S, Ichimura M, Kuwahara T
    • Journal Title

      J Med Invest 57

      Pages: 275-283

    • NAID

      130004465219

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular genetic investigations of contaminated contact lens storage cases as reservoirs for Pseudomonas aeruginosa keratitis.2010

    • Author(s)
      Ogushi Y, Eguchi H, Kuwahara T, Hayabuchi N, Kawabata M.
    • Journal Title

      Jpn J Ophthalmol 54

      Pages: 550-554

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient electrotransformation of Bacteroides fragilis.2010

    • Author(s)
      Ichimura M, Nakayama-Imaohji H, Wakimoto S, Morita H, Hayashi T, Kuwahara T
    • Journal Title

      Appl Environ Microbiol 76

      Pages: 3325-3332

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient electrotransformation of Bacteroides fragilis.2010

    • Author(s)
      Ichimura M, et al
    • Journal Title

      Appl.Environ.Microbiol.

      Volume: 76 Pages: 3325-3332

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular genetic investigations of contaminated contact lens storage cases as reservoirs for Pseudomonas aerulinosa keratitis2010

    • Author(s)
      Ogushi Y, et al.
    • Journal Title

      Jpn.J.Ophthalmol.

      Volume: 54 Pages: 550-554

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient electrotransformation of Bacteroides fragilis2010

    • Author(s)
      Ichimura M, et al.
    • Journal Title

      Appl.Environ.Microbiol. 76(in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] コリネバクテリウムが起炎菌と考えられた感染性角膜炎の1例2009

    • Author(s)
      稲田耕大、前田郁世、池田欣史、宮崎大、井上幸次、江口洋、塩田洋、桑原知巳
    • Journal Title

      あたらしい眼科 26

      Pages: 1105-1107

    • NAID

      10026255979

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] ペニシリン耐性肺炎球菌結膜炎の1例.2009

    • Author(s)
      江口洋、桑原知巳、大木武夫、塩田洋、田中真理子、田中健
    • Journal Title

      あたらしい眼科 26

      Pages: 376-378

    • NAID

      10026254668

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the site-specific DNA invertase responsible for the phase variation of SusC/SusD family outer membrane proteins in Bacteroides fragilis.2009

    • Author(s)
      Nakayama-Imaohji H, Hirakawa H, Ichimura M, Wakimoto S, Kuhara S, Hayashi T, Kuwahara T
    • Journal Title

      J Bacteriol 191

      Pages: 6003-6011

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of apoptosis-inducing substances from soybean protein by Clostridium butyricum : characterization of their toxic effects on human colon carcinoma cells.2009

    • Author(s)
      Arimochi H, Morita K, Nakanishi S, Kataoka K, Kuwahara T
    • Journal Title

      Cancer Lett 277

      Pages: 190-198

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic characterization of type A enterotoxigenic Clostridium perfringens strains.2009

    • Author(s)
      Deguchi A, Miyamoto K, Kuwahara T, Miki Y, Kaneko I, Li J, McClane BA, Akimoto S.
    • Journal Title

      PLos One 4

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Population structure and distribution of virulence-related genes of Bacteroides fragilis isolates from Korea and Japan.2009

    • Author(s)
      Ko KS, Kuwahara T, Lee K, Kook YH.
    • Journal Title

      Diagn Microbiol Infect Dis 64

      Pages: 340-343

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic characterization of type A enterotoxigenic Clostridium perfringens strains2009

    • Author(s)
      Deguchi A, et al.
    • Journal Title

      PLoS One 4

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the site-specific DNA invertase responsible for the phase variation of SusC/SusD family outer membrane proteins in Bacteroides fragilis.2009

    • Author(s)
      Nakayama-Imaohji H, et al.
    • Journal Title

      J.Bacteriol. 191

      Pages: 6003-6011

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of apoptosis-inducing substances from soybean protein by Clostridium butyricum : characterization of their toxic effects on human colon carcinoma cells.2009

    • Author(s)
      Arimochi H, et al.
    • Journal Title

      Cancer Lett. 277

      Pages: 190-198

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effects of fermented brown rice on induction of acute colitis by dextran sulfate sodium in rats.2008

    • Author(s)
      Kataoka K, Ogasa S, Kuwahara T, Bando Y, Hagiwara M, Arimochi H, Nakanishi S, Iwasaki T, Ohnishi Y.
    • Journal Title

      Dig Dis Sci 53

      Pages: 1601-1608

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effect of fermented brown rice on induction of acute colitis by dextran sulfate sodium in rats.2008

    • Author(s)
      Kataoka K, et al.
    • Journal Title

      Dig. Dis. Sci. 53

      Pages: 1601-1608

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 難培養性腸内細菌ゲノムへのアプローチー2010

    • Author(s)
      桑原知巳
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genomic analysis of unculturable gut microbe,「超高速ゲノム解読時代における細菌学研究」2010

    • Author(s)
      桑原知巳
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation]2010

    • Author(s)
      脇本信, 今大路治之, 市村穣, 桑原知巳, 安友康二
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Bacteroides fragilisの形質転換法に関する検討2009

    • Author(s)
      桑原知巳
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Bacteroides fragilis における外膜vesicle形成と莢膜発現との関連性2009

    • Author(s)
      桑原知巳
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒト常在フローラ研究の最前線2008

    • Author(s)
      桑原知巳
    • Organizer
      第82回日本感染症学会総会「ゲノムからみた感染症」
    • Place of Presentation
      松江
    • Related Report
      2010 Final Research Report
  • [Book] 難培養微生物研究の最新技術II(大熊盛也、工藤俊章監修)(ヒト腸内フローラのメタゲノム解析と肥満・健康.)2010

    • Author(s)
      桑原知巳、林哲也
    • Publisher
      CMC出版、東京
    • Related Report
      2010 Final Research Report
  • [Book] 医科細菌学第4版(笹川千尋、林哲也編)(常在細菌叢.)2008

    • Author(s)
      桑原知巳
    • Publisher
      南江堂、東京
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi