Development of innovative genetic manipulation methods of Bifidobacterium based on the genome information.

• Project/Area Number: 20510189
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: Gifu University
• Principal Investigator: SUZUKI Tohru Gifu University, 連合農学研究科, 教授 (20235972)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 形質転換 / プラスミド人工修飾法 / PAM / 遺伝子破壊 / Bifidobacterium adolescentis / 相同組換え / 逆遺伝学 / Bifidobacterium longum / 温度感受性プラスミド / ビフィズス菌 / プロバイオティクス / テーラーメードプロバイオティクス / ゲノム解析 / ゲノミクス / 腸内細菌 / 遺伝子操作 / 制限修飾系 / ゲノム情報 / PAM法 / エレクトロポレーション / Plasmid Artificial Modification

Research Abstract

Bifidobacteria are thought to be important bacteria for human health. However, the scientific evidences has not yet obtained because it has been difficult to manipulate the gene. In this study, we developed new transformation method, selection marker and gene knock-out method based on the genome analyses.

Research Products

• [Journal Article] Bifidobacteria can protect from enteropathogenic infection through production of acetate. (2011)
  • Author(s): Fukuda, S., H.Toh, K.Hase, K.Oshima, Y.Nakanishi, K.Yoshimura, T.Tobe, J.M.Clarke, D.L.Topping, T.Suzuki, T.D.Taylor, K.Itch, J.Kikuchi, H.Morita, M.Hattori, H.Ohno.
  • Journal Title: Nature 469 Pages: 543-547
  • Peer Reviewed
• [Journal Article] Bifidobacteria can protect from enteropathogenic infection through production of acetate. (2011)
  • Author(s): Fukuda, S., H.Toh, K.Hase, K.Oshima, Y.Nakanishi, K.Yoshimura, T.Tobe, J.M.Clarke, D.L.Topping, T.Suzuki, T.D.Taylor, K.Itoh, J.Kikuchi, H.Morita, M.Hattori, H.Ohno.
  • Journal Title: Nature Volume: 469 Pages: 543-547
  • Peer Reviewed
• [Journal Article] Characterization of an O-desmethylangolensin-producing bacterium isolated from human feces. (2010)
  • Author(s): Yokoyama, S., T.Niwa, T.Osawa, T.Suzuki.
  • Journal Title: Arch.Microbial.(DOI 10.1007/s00203- 009-0524-5) 192 Pages: 15-22
  • Peer Reviewed
• [Journal Article] Characterization of an O-desmethylangolensin-producing bacterium isolated from human feces. (2010)
  • Author(s): Yokoyama, S., T.Niwa, T.Osawa, T.Suzuki.
  • Journal Title: Arch.Microbiol. Volume: 192 Pages: 15-22
  • Peer Reviewed
• [Journal Article] The multiplicity of N-glycan structures of bovine milk 18 kDa lactophorin (miGlyCAM-1). (2010)
  • Author(s): Inagaki, M., S.Nakaya, D.Nohara, T.Yabe, Y.Kanamaru, T.Suzuki.
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 74 Pages: 447-450
  • Peer Reviewed
• [Journal Article] Characterization of an O-desmethylangolensin-producing bacterium isolated from human feces (2010)
  • Author(s): Yokoyama, S.-i., T.Niwa, T.Osawa, T.Suzuki
  • Journal Title: Arch.Microbiol 192(●)
  • Peer Reviewed
• [Journal Article] Intra-species Diversity Between Seven Bifidobacterium adolescentis Strains Identified by Genome-wide Tiling Array Analysis. (2009)
  • Author(s): Yasui, K., M.Tabata, S.Yamada, T.Abe, T.Ikemura, R.Osawa, T.Suzuki.
  • Journal Title: Biosci.Biotechnol. Biochem. 73 Pages: 1422-1424
  • NAID: 10027542817
  • Peer Reviewed
• [Journal Article] Improvement of bacterial transformation efficiency using plasmid artificial modification. (2009)
  • Author(s): Yasui, K., Y.Kano, K.Tanaka, K.Watanabe, M.Shimizu-Kadota, H.Yoshikawa, T.Suzuki.
  • Journal Title: Nucleic Acids Res doi:10.1093/nar/gkn884 Pages: 37-37
  • Peer Reviewed
• [Journal Article] Improvement of bacterial transformation efficiency using plasmid arti ficial modifi cation. (2009)
  • Author(s): Yasui K, Kano Y, Tanaka K, Watanabe K, Shimizu-Kadota M, Yoshikawa H, Suzuki T.
  • Journal Title: Nucleic Acids Res. 37(e3. Epub)
  • Peer Reviewed
• [Journal Article] Intra-species diversity between seven Bifidobacterium adolescentis strains identified by genome-wide tiling array analysis (2009)
  • Author(s): Yasui, Kazumasa., Tabata Mototsugu., Yamada, Satoki., Osawa, Ro., Suzuki, Tohru
  • Journal Title: Bioscience, Biotechnology, and Biochemistry (In Press)
  • NAID: 10027542817
  • Peer Reviewed
• [Journal Article] Isolation and characterization of a novel equol-producing bacterium from human feces. (2008)
  • Author(s): Yokoyama, S.-I., T.Suzuki.
  • Journal Title: Biosci.Biotechnol.Biochem. 72 Pages: 2660-2666
  • NAID: 10027533138
  • Peer Reviewed
• [Presentation] Expression of catalase gene affords toleranceto oxygen in Bifidobacterium longum 105 A. (2010)
  • Author(s): Jianlong, H., T.Suzuki
  • Organizer: in 3rd Intnatinal Symposium on Propionibacteria and Bifidobacteria.
  • Place of Presentation: Oviedo, Spain.
• [Presentation] Construction and molecular characterization of the temperature sensitive plasmid for Bifidobacterium longum. (2010)
  • Author(s): 坂口広太, 鈴木徹
  • Organizer: in 3rd Intnatinal Symposium on Propionibac teria and Bifidobacteria.
  • Place of Presentation: Oviedo, Spain.
• [Presentation] ダイズイソフラボンをEquolに変換するEggerthella sp.YY7918のゲノム解析 (2010)
  • Author(s): 横山慎一郎, 大島健志朗, 野村泉, 鈴木徹
  • Organizer: in農芸化学会大会
  • Place of Presentation: 東京大学駒場キヤンパス
• [Presentation] ダイズイソフラボンをO-DMAに変換するヒト腸内由来の新規Clostridium Cluster XIVa細菌SY8519株のゲノム解析 (2010)
  • Author(s): 横山慎一郎, 野村泉, 鈴木徹, 大島健志朗, 服部正平
  • Organizer: in日本乳酸菌学会2010年度大会
  • Place of Presentation: フォレスト仙台
• [Presentation] 牛乳ラクトフォリン(milk Glycam-1)のN型糖鎖構造の多様性 (2010)
  • Author(s): 稲垣瑞穂, 中家修一, 野原大輔, 矢部富雄, 金丸義敬, 鈴木徹
  • Organizer: in農芸化学会大会
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] Bifidobacterium longumで機能するプロモーターの活性比較 (2010)
  • Author(s): 賀建龍, 鈴木徹
  • Organizer: in農芸化学会大会
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] Construction and molecular characterization of the temperature sensitive plasmid for Buidobacterium longum. (2010)
  • Author(s): K.Sakaguchi, T.Suzuki
  • Organizer: 3^<rd> Intnatinal Symposium on Propionibacteria and Bifidobacteria
  • Place of Presentation: Oviedo, Spain
• [Presentation] Expression of catalase gene affords toleranceto oxygen in Buidobacterium longum 105-A (2010)
  • Author(s): HE Jianlong, T.Suzuki
  • Organizer: 3^<rd> Intnatinal Symposium on Propionibacteria and Bifidobacteria
  • Place of Presentation: Oviedo, Spain
• [Presentation] プラスミ人工修飾法(PAM)によるビフィズス菌の遺伝子導入系の開発 (2009)
  • Author(s): 安井一将, 坂口広太, 加納康正, 門多真理子, 吉川博文, 鈴木徹
  • Organizer: in第13回腸内細菌学会
  • Place of Presentation: 北里大学薬学部,東京.
• [Presentation] Aspergillus oryzae のキシリトール代謝関連遺伝子削除株によるキシリトール生産 (2009)
  • Author(s): 榊原由佳, 渥美元規, 中村浩平, 北本則行, 鈴木徹, 高見澤一裕
  • Organizer: in生物工学会61回大会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] 枯草菌を用いたPAM(Plasmid Artificial Modification)法による乳酸球菌Lactococcus lactis IO-1株の形質転換頻度の改善 (2009)
  • Author(s): 渡辺寛, 廣崎辰也, 東崎正, 鈴木徹, 園元謙二, 門多真理子, 吉川博文.
  • Organizer: in日本乳酸菌学会2009年度大会
  • Place of Presentation: ベルクラシック甲府
• [Presentation] A repeatable gene disruption method for Bifidobacterium longum 105-A (2008)
  • Author(s): Sakaguchi K., N.Funaoka, Y.Kano, T.Suzuki
  • Organizer: in 9th Symposoum on Lactic Acid Bacteria
  • Place of Presentation: Egmond, Netherlands : FEMS.
• [Presentation] Complete genome structure of Bifidobacterium adolescentis ATCC15703 and inter strain genome-wide polymorphism between 7 B.adolescentis strains using tilling array analyses. (2008)
  • Author(s): Suzuki T., H.Tabata, K.Suganuma, K.Yasui, K.Tanaka, K.Watanabe, K.Sakaguchi, T.Abe, T.Ikemura, R.Osawa
  • Organizer: in 9th Symposoum on Lactic Acid Bacteria.
  • Place of Presentation: Egmond, Netherlands : FEMS.
• [Presentation] Plasmid artificial modification (PAM) : a genomic information-based method to improve transformation efficiency in Bifidobacterium adolescentis ATCC15703. (2008)
  • Author(s): 安井一将, 加納康正, 鈴木徹
  • Organizer: in 9th Symposoum on Lactic Acid Bacteria.
  • Place of Presentation: Egmond Netherlands : FEMS.
• [Presentation] プラスミド人工的メチル化(PAM)によるBifidobacterium adolescentis ATCC15703の形質転換効率の向上 (2008)
  • Author(s): 安井一将, 鈴木徹, 加納康正
  • Organizer: in日本乳酸菌学会2008年度大会
  • Place of Presentation: 京都大学百周年時計台記念館
• [Presentation] ヒト糞便由来の新規Equol産生菌の単離と諸性質について (2008)
  • Author(s): 横山慎一郎, 鈴木徹
  • Organizer: in生物工学会60回大会
  • Place of Presentation: 東北学院大学土樋キャンパス
• [Presentation] タイリングアレイと自己組織化マップを用いたBifidobacterium adolescentis 菌株間のゲノム多型の網羅的解析 (2008)
  • Author(s): 鈴木徹, 田端大嗣, 菅沼宏美, 安井一将, 田中香お里, 渡邉邦友, 池村淑道, 阿部貴志, 大澤朗.
  • Organizer: in第12回腸内細菌学会
  • Place of Presentation: 東京大学弥生講堂
• [Book] 染色体 DNA の遺伝解析.世紀を超えるビフィズス菌の研究 その基礎と臨床応用から 製品開発へー (2011)
  • Author(s): 鈴木徹
  • Publisher: 日本ビフィズス菌センタ ー
• [Book] Strain Engineering : Methods and Protocols, Methods in Molecular Biology(Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria.)(In J.A.Williams (ed.)) (2011)
  • Author(s): Suzuki T, K.Yasui
  • Publisher: A Humana Press.
• [Book] Plasmid Artificial Modification : A Novel Method for Efficient DNA Transfer into Bacteria.(J.A.Williams (ed.)), Strain Engineering : Methods and Protocols, Methods in Molecular Biology (2011)
  • Author(s): Suzuki, T., K.Yasui.
  • Total Pages: 21
  • Publisher: A Humana Press, Springer
• [Book] Lactic Acid Bacteria : Sophisticated Progress(Current status of Bifidobacterium gene maniplation technology.)(In K.Sonomoto and A.Yokota (ed.)) (2010)
  • Author(s): Fukiya, S., Suzuki T, Y.Kano, A.Yokota.
  • Publisher: Hrizon Scientific Press, UK.
• [Book] Current status of Bifidobacterium gene maniplation technology.(K.Sonomoto and A(ed.)), Lactic Acid Bacteria : Sophisticated Progress (2010)
  • Author(s): Fukiya, S., T.Suzuki, Y.Kano, Yokota.
  • Total Pages: 20
  • Publisher: Hrizon Scientific Press, UK.
• [Remarks] ホームページ等