Development of innovative genetic manipulation methods of Bifidobacterium based on the genome information.
Project/Area Number |
20510189
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied genomics
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Research Institution | Gifu University |
Principal Investigator |
SUZUKI Tohru Gifu University, 連合農学研究科, 教授 (20235972)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 形質転換 / プラスミド人工修飾法 / PAM / 遺伝子破壊 / Bifidobacterium adolescentis / 相同組換え / 逆遺伝学 / Bifidobacterium longum / 温度感受性プラスミド / ビフィズス菌 / プロバイオティクス / テーラーメードプロバイオティクス / ゲノム解析 / ゲノミクス / 腸内細菌 / 遺伝子操作 / 制限修飾系 / ゲノム情報 / PAM法 / エレクトロポレーション / Plasmid Artificial Modification |
Research Abstract |
Bifidobacteria are thought to be important bacteria for human health. However, the scientific evidences has not yet obtained because it has been difficult to manipulate the gene. In this study, we developed new transformation method, selection marker and gene knock-out method based on the genome analyses.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Bifidobacteria can protect from enteropathogenic infection through production of acetate.2011
Author(s)
Fukuda, S., H.Toh, K.Hase, K.Oshima, Y.Nakanishi, K.Yoshimura, T.Tobe, J.M.Clarke, D.L.Topping, T.Suzuki, T.D.Taylor, K.Itch, J.Kikuchi, H.Morita, M.Hattori, H.Ohno.
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Journal Title
Nature 469
Pages: 543-547
Related Report
Peer Reviewed
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[Journal Article] Bifidobacteria can protect from enteropathogenic infection through production of acetate.2011
Author(s)
Fukuda, S., H.Toh, K.Hase, K.Oshima, Y.Nakanishi, K.Yoshimura, T.Tobe, J.M.Clarke, D.L.Topping, T.Suzuki, T.D.Taylor, K.Itoh, J.Kikuchi, H.Morita, M.Hattori, H.Ohno.
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Journal Title
Nature
Volume: 469
Pages: 543-547
Related Report
Peer Reviewed
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[Journal Article] Intra-species diversity between seven Bifidobacterium adolescentis strains identified by genome-wide tiling array analysis2009
Author(s)
Yasui, Kazumasa., Tabata Mototsugu., Yamada, Satoki., Osawa, Ro., Suzuki, Tohru
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Journal Title
Bioscience, Biotechnology, and Biochemistry (In Press)
NAID
Related Report
Peer Reviewed
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[Presentation] Complete genome structure of Bifidobacterium adolescentis ATCC15703 and inter strain genome-wide polymorphism between 7 B.adolescentis strains using tilling array analyses.2008
Author(s)
Suzuki T., H.Tabata, K.Suganuma, K.Yasui, K.Tanaka, K.Watanabe, K.Sakaguchi, T.Abe, T.Ikemura, R.Osawa
Organizer
in 9th Symposoum on Lactic Acid Bacteria.
Place of Presentation
Egmond, Netherlands : FEMS.
Related Report
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