| Project/Area Number |
20510190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Shizuoka University |
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Principal Investigator |
MOTOHASHI Reiko Shizuoka University, 農学部, 准教授 (90332296)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | プラスチド / クロモプラスト / プロテオーム / トマト |
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| Research Abstract |
We are analyzing the differentiation mechanism of plastids in tomato using the proteomic techniques as follows.
First, to analyze the comprehensive proteome involved in chloroplast to chromoplast differentiation, we established methods to isolate and identify chromoplast proteins of Micro-Tom fruits at four developmental stages (mature green, yellow, orange and red). We identified approximately 440 plastid proteins using LC-MS/MS.
Second, we are trying to collect natural variations and mutants that have various colors of fruits such as white, black and orange, and then stop their ripening at the intermediate stage. We compare the chromoplast proteome in these fruits with that of Micro-Tom fruits at four developmental stages using two-dimensional gel electrophoresis, and find specific proteins related to chromoplast development, ripening, and the fruits color.
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