A search of the key proteins related chromoplast differentiation and an elucidation of the mechanism.
| Project/Area Number |
20510190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Shizuoka University |
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Principal Investigator |
MOTOHASHI Reiko Shizuoka University, 農学部, 准教授 (90332296)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | プラスチド / クロモプラスト / プロテオーム / トマト |
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| Research Abstract |
We are analyzing the differentiation mechanism of plastids in tomato using the proteomic techniques as follows.
First, to analyze the comprehensive proteome involved in chloroplast to chromoplast differentiation, we established methods to isolate and identify chromoplast proteins of Micro-Tom fruits at four developmental stages (mature green, yellow, orange and red). We identified approximately 440 plastid proteins using LC-MS/MS.
Second, we are trying to collect natural variations and mutants that have various colors of fruits such as white, black and orange, and then stop their ripening at the intermediate stage. We compare the chromoplast proteome in these fruits with that of Micro-Tom fruits at four developmental stages using two-dimensional gel electrophoresis, and find specific proteins related to chromoplast development, ripening, and the fruits color.
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Report
(4 results)
Research Products
(60 results)
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[Journal Article] LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis.2010
Author(s)
Tanaka R, Rothbart M, Oka S, Takabayashi A, Takahashi K, Shibata M, Myouga F, Motohashi R, Shinozaki K, Grimm B, Tanaka A.
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Journal Title
Proc.Natl.Acad.Sci.USA 107(38)
Pages: 1672-16725
Related Report
Peer Reviewed
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[Journal Article] Plant-Growth Regulator, Imidazole-4-Carboxamide, Produced by the Fairy Ring Forming Fungus Lepista sordida.2010
Author(s)
Choi JH, Abe N, Tanaka H, Fushimi K, Nishina Y, Morita A, Kiriiwa Y, Motohashi R, Hashizume D, Koshino H, Kawagishi H.
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Journal Title
J.Agric.Food.Chem. 58
Pages: 9956-9959
Related Report
Peer Reviewed
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[Journal Article] Disclosure of the "fairy" of fairy-ring-forming fungus Lepista sordida.2010
Author(s)
Choi JH, Fushimi K, Abe N, Tanaka H, Maeda S, Morita A, Hara M, Motohashi R, Matsunaga J, Eguchi Y, Ishigaki N, Hashizume D, Koshino H, Kawagishi H.
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Journal Title
Chembiochem. 11(10)
Pages: 1373-1377
Related Report
Peer Reviewed
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[Journal Article] Disclosure of the "fairy" of fairy-ring-forming fungus Lepista sordida.2010
Author(s)
Choi JH, Fushimi K, Abe N, Tanaka H, Maeda S, Morita A, Hara M, Motohashi R, Matsunaga J, Eguchi Y, Ishigaki N, Hashizume D, Koshino H, Kawagishi H.
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Journal Title
Chemhinchem.
Volume: 11
Pages: 1373-1377
Related Report
Peer Reviewed
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[Journal Article] Plant-growth regulator, imidazole-4-carboxamide, produced by the fairy ring forming fungus Lepista sordida.2010
Author(s)
Choi JH, Abe N, Tanaka H, Fushimi K, Nishina Y, Morita A, Kiriiwa Y, Motohashi R, Hashizume D, Koshino H, Kawagishi H.
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Journal Title
J Agric Food Chem.
Volume: 58
Pages: 9956-9959
Related Report
Peer Reviewed
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[Journal Article] LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis.2010
Author(s)
Tanaka R, Rothbart M, Oka S, Takabayashi A, Takahashi K, Shibata M, Myouga F, Motohashi R, Shinozaki K, Grimm B, Tanaka A.
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Journal Title
Proc Natl Acad Sci USA.
Volume: 107
Pages: 16721-16725
Related Report
Peer Reviewed
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[Journal Article] The pentatricopeptide repeat protein OTP82 is required for RNA editing of plastid ndhB and ndhG transcripts2010
Author(s)
Okuda, Kenji, Hammani, Kamel, Tanz, Sandra, Peng, Lianwei, Fukao, Yoichiro, Myouga, Fumiyoshi, Motohashi, Rieko, Shinozaki, Kazuo, Small, Ian, Shikanai, Toshiharu
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Journal Title
Plant J 61
Pages: 339-349
Related Report
Peer Reviewed
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[Journal Article] The Chloroplast Function Database : a large-scale collection of Arabidopsis Ds/Spm- or T-DNA-tagged homozygous lines for nuclear-encoded chloroplast proteins and their systematic phenotype analysis
Author(s)
Myouga, Fumiyoshi, Akiyama, Kenji, Motohashi, Rieko, Kuromori, Takashi, Ito, Takuya, Iizumi, Haruko, Ryusui, Rie, Sakurai, Tetsuya, Shinozaki, Kazuo
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Journal Title
Related Report
Peer Reviewed
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