Construction of novel RNAi vectors for analysis of fruiting body-related genes in edible basidiomycete fungi Lentinula edodes by reverse genetic method.

• Project/Area Number: 20510193
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: Kitami Institute of Technology
• Principal Investigator: SATO Toshitsugu Kitami Institute of Technology, 工学部, 准教授 (00390881)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 食用担子菌 / シイタケ / RNAiベクター / 遺伝子発現抑制 / 子実体関連遺伝子 / 遺伝子 / 発現制御 / 子実体 / プロモータ

Research Abstract

To analyze genes related fruiting body, we constructed novel RNAi vectors for Lenitnula edodes. Novel RNAi vectors carry two convergent opposing promoters, and a target gene between promoters. Independent transcription of a target gene from each promoter would produce a sense and antisense RNAs in the cell, which would induce RNAi. It is expected that the obtained vectors are useful for the function analysis of fruiting body related genes.

Research Products

• [Journal Article] Gene silencing of Lentinula edodes lcc1 gene by expression of homologous inverted repeat sequence. (2011)
  • Author(s): Keiko Nakade, Yuichi Sakamoto, Hisayuki Watanabe, Toshitsugu Sato
  • Journal Title: Microbiol Res (in press)
  • Peer Reviewed
• [Journal Article] Construction of novel vectors for transformation of Lentinula edodes using chitin synthase gene promoter. (2011)
  • Author(s): Toshitsugu Sato, Kumiko Okawa, Tatsuya Hirano
  • Journal Title: J Biosci Bioeng 111 Pages: 117-120
  • NAID: 110008460321
  • Peer Reviewed
• [Journal Article] Construction of novel vectors for transformation of Lentinula edodes using a chitin svnthase gene promoter. (2011)
  • Author(s): Toshitsugut Sato
  • Journal Title: Journal Bioscience Bioengineering Volume: 111 Pages: 117-120
  • Peer Reviewed
• [Journal Article] Gene silencing of Lentinula edodes lcc1 gene by expression of homologo us inverted repeat sequence. (2011)
  • Author(s): Keiko Nakade
  • Journal Title: Microbiological Research Volume: (in press)
  • Peer Reviewed
• [Journal Article] Cloning and characterization of Lentinula edodes class II chitin synthase gene (2010)
  • Author(s): Toshitsugu Sato, Kumiko Okawa, Tatsuya Hirano
  • Journal Title: LeChs2. Biosci Biotechnol Biochem 74(8) Pages: 1707-1709
  • Peer Reviewed
• [Journal Article] Cloning and characterization of Lentinula edodes class II chitin synthase gene, LeCbs2. (2010)
  • Author(s): Toshitsugu Sato
  • Journal Title: Bioscience Biotechnology Biochemistry Volume: 74 Pages: 1707-1709
  • Peer Reviewed
• [Journal Article] PCR subtraction, Characterization of the post-harvest changes in gene transcription in the gill of the Lentinula edodes fruiting body. (2009)
  • Author(s): Yuichi Sakamoto, Keiko Nakade, Toshitsugu Sato
  • Journal Title: Curr Genet 55 Pages: 409-423
  • Peer Reviewed
• [Journal Article] シイタケの形質転換と子実体収穫後に発現する遺伝子 (2009)
  • Author(s): 佐藤利次
  • Journal Title: バイオサイエンスとインダストリー 67巻 Pages: 316-319
  • NAID: 10026194776
• [Journal Article] Characterization of the post-harvest changes in gene transcription in the gill of the Lentinula edodes fruiting body. (2009)
  • Author(s): Yuichi Sakamoto
  • Journal Title: Current Genetics 55 Pages: 409-423
  • Peer Reviewed
• [Journal Article] シイタケの形質転換と子実体収穫後に発現する遺伝子 (2009)
  • Author(s): 佐藤利次
  • Journal Title: バイオサイエンスとインダストリー 67 Pages: 316-319
  • NAID: 10026194776
• [Presentation] Establishment of RNAi method in Lentinula edodes. (2009)
  • Author(s): 中出, ら
  • Organizer: 25th Fungal Genetics Conference
  • Place of Presentation: Asilomar, CA, USA
• [Presentation] Characterization of genes expressed after harvesting of the Lentinula edodes fruiting body. Establishment of RNAi method in Lentinula edodes (2008)
  • Author(s): 坂本, ら, 中出, ら
  • Organizer: Genetics and Cellular Biology of Basidiomy cetes VII 25th Fungal Genetics Conference
  • Place of Presentation: Southeast Missouri Univ., MI, USA Asilomar, CA, USA
• [Remarks] ホームページ等
  • URL: http://www.chem.kitami-it.ac.jp/laboratory/satou/
• [Remarks]
  • URL: http://koho2.office.kitami-it.ac.jp/cgi-bin/WebObjects/souran.woa/2/wo/YbXmK3P1LItjg7B9sBdA5w/1.1.19.8.1.0
• [Patent(Industrial Property Rights)] プロモーター遺伝子及び発現ベクター (2010)
  • Inventor(s): 佐藤利次、齋藤久美子、平野達也、江井仁
  • Industrial Property Rights Holder: 岩手県
  • Acquisition Date: 2010-10-22
• [Patent(Industrial Property Rights)] プロモーター遺伝子及び発現ベクター (2010)
  • Inventor(s): 佐藤利次
  • Industrial Property Rights Holder: 岩手県
  • Acquisition Date: 2010-10-22