Construction of novel RNAi vectors for analysis of fruiting body-related genes in edible basidiomycete fungi Lentinula edodes by reverse genetic method.
| Project/Area Number |
20510193
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Kitami Institute of Technology |
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Principal Investigator |
SATO Toshitsugu Kitami Institute of Technology, 工学部, 准教授 (00390881)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 食用担子菌 / シイタケ / RNAiベクター / 遺伝子発現抑制 / 子実体関連遺伝子 / 遺伝子 / 発現制御 / 子実体 / プロモータ |
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| Research Abstract |
To analyze genes related fruiting body, we constructed novel RNAi vectors for Lenitnula edodes. Novel RNAi vectors carry two convergent opposing promoters, and a target gene between promoters. Independent transcription of a target gene from each promoter would produce a sense and antisense RNAs in the cell, which would induce RNAi. It is expected that the obtained vectors are useful for the function analysis of fruiting body related genes.
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Report
(4 results)
Research Products
(16 results)
