A new approach for sample preparation : expression and stable-isotope labeling of mature proteins using plant cells
| Project/Area Number |
20510198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
OHKI Shinya Japan Advanced Institute of Science and Technology, ナノマテリアルテクノロジーセンター, 准教授 (70250420)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Masashi 石川県立大学, 生物資源工学研究所, 准教授 (00320911)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | タンパク質 / 生体分子 / バイオテクノロジー / ウイルス / 植物 / 植物細胞 / タンパク質発現 / ウィルスベクター / 構造生物化学 / NMR / 安定同位体標 |
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| Research Abstract |
We developed a novel protein expression method using plant cells and virus vector. We prepared a protein, which cannot be expressed with popular E.coli systems. We established a protocol for preparing the stable-isotope labeled proteins with this system. To show the workability, we performed protein expression, stable-isotope labeling, and structure determination of a protein, of which expression is tough. In summary, this work had suggested a new method, which has a potential to study challenging proteins, to prepare samples, of which expression is known as difficult.
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Report
(4 results)
Research Products
(21 results)
