• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Detailed measurements of protein dynamics in crystals

Research Project

Project/Area Number 20510199
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionKyoto University

Principal Investigator

TAKEDA Kazuki  Kyoto University, 大学院・理学研究科, 講師 (30332290)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsタンパク質 / 結晶 / スペクトル
Research Abstract

In order to validate structural changes of proteins in the crystalline state, I constructed a microspectrometer which can measure spectra of crystalline samples with high optical densities. Spectra of various pigment-containing proteins were measured from flat crystals suitable for measurements. Influences of X-rays were precisely estimated in order to derive meaningful information from the crystallographic results. In addition, a novel protein structure involved in the photosynthesis was successfully determined.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (17 results)

All 2011 2010 2009 2008 Other

All Journal Article (10 results) (of which Peer Reviewed: 6 results) Presentation (4 results) Remarks (3 results)

  • [Journal Article] Dimer structure and conformational variability in the N-terminal region of an archeal small heat shock protein.2011

    • Author(s)
      K.Takeda, T.Hayashi, T.Abe, Y.Hirano, Y.Hanazono, M.Yohda, K.Miki
    • Journal Title

      J.Struct. Biol. 174

      Pages: 92-99

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 超高分解能・超高精度のタンパク質結晶構造解析2011

    • Author(s)
      竹田一旗、三木邦夫
    • Journal Title

      生物物理 51

      Pages: 96-99

    • NAID

      10028105855

    • Related Report
      2010 Final Research Report
  • [Journal Article] Dimer structure and conformational variability in the N-terminal region of an archeal small heat shock protein2011

    • Author(s)
      K.Takeda, T.Hayashi, T.Abe, Y.Hirano, Y.Hanazono, M.Yohda, K.Miki
    • Journal Title

      J.Struct.Biol.

      Volume: 174 Pages: 92-99

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 超高分解能・超高精度のタンパク質結晶構造解析2011

    • Author(s)
      竹田一旗, 三木邦夫
    • Journal Title

      生物物理

      Volume: 51 Pages: 96-99

    • NAID

      10028105855

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Detailed assessment of X-ray induced structural perturbation in a crystalline state protein.2010

    • Author(s)
      K.Takeda, K.Kusumoto, Y.Hirano, K.Miki
    • Journal Title

      J.Struct. Biol. 169

      Pages: 135-144

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] タンパク質における超高分解能X線結晶構造解析2010

    • Author(s)
      竹田一旗、平野優、三木邦夫
    • Journal Title

      日本結晶学会誌 52

      Pages: 14-18

    • NAID

      10026107733

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Journal Article] Detailed assessment of X-ray induced structural perturbation in a crystalline state protein.2010

    • Author(s)
      K.Takeda, K.Kusumoto, Y.Hirano, K.Miki
    • Journal Title

      J.Struct.Biol. 169

      Pages: 135-144

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate.2009

    • Author(s)
      N.Akiyama, K.Takeda, K.Miki
    • Journal Title

      J.Mol.Biol. 192

      Pages: 559-565

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate.2009

    • Author(s)
      N.Akiyama, K.Takeda, K.Miki
    • Journal Title

      J.Mol.Biol. 392

      Pages: 559-565

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] タンパク質研究を躍進させたX線結晶構造解析2008

    • Author(s)
      竹田一旗, 三木邦夫
    • Journal Title

      化学 63

      Pages: 25-28

    • Related Report
      2008 Annual Research Report
  • [Presentation] タンパク質の価電子を見る2010

    • Author(s)
      竹田一旗
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-18
    • Related Report
      2010 Final Research Report
  • [Presentation] タンパク質の価電子を見る2010

    • Author(s)
      竹田一旗
    • Organizer
      第10回 日本蛋白質科学会年会
    • Place of Presentation
      札幌市(招待講演)
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超高分解能構造解析---タンパク質における電子軌道形状の可視化をめざして2009

    • Author(s)
      竹田一旗
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本市
    • Year and Date
      2009-05-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 超高分解能構造解析---タンパク質における電子軌道形状の可視化をめざして2009

    • Author(s)
      竹田一旗
    • Organizer
      第9回 日本蛋白質科学会年会
    • Place of Presentation
      熊本市
    • Year and Date
      2009-05-20
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.kuchem.kyoto-u.ac.jp/organization/member/ktakeda.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.kuchem.kyoto-u.ac.jp/organization/member/ktakeda.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.kuchem.kyoto-u.ac.jp/organization/member/ktakeda.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi