| Project/Area Number |
20510209
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Living organism molecular science
|
|---|
| Research Institution | Hyogo University of Health Sciences |
|---|
Principal Investigator |
AOKI Shunji Hyogo University of Health Sciences, 薬学部, 教授 (60252699)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
FURUKAWA Tatsuhiko 鹿児島大学, 大学院・医歯学総合研究科, 教授 (40219100)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 内因性血管新生阻害因子 / プロモーター活性化 / thrombospondin-1 / ヒト齊帯静脈血管内皮細胞 / 腫瘍血管新生 |
|---|
| Research Abstract |
In this study, we attempt to find the natural compounds which activate the promoter of "endogenous anti-angiogenesis factor" such as endostatin, angiostatin and thrombospondin. We tried to establish a bioassay method using the cells that were stably transfected by the luciferase reporter plasmid fused with the promoter domain of the thrombospondin-1 gene. However, We couldn't achieve to establish the stably transfected cells. On the other hand, on the guidance of selective growth inhibition against human umbilical vein endothelial cells (HUVECs), we searched for anti-angiogenic substances from extracts of medicinal plants. We found several active extract and tried to isolate the active components by the assay-guided separation.
|
