Search of natural compound activating endogenous anti-angiogenesis factor as an anti-cancer agent.

• Project/Area Number: 20510209
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Hyogo University of Health Sciences
• Principal Investigator: AOKI Shunji Hyogo University of Health Sciences, 薬学部, 教授 (60252699)
• Co-Investigator(Renkei-kenkyūsha): FURUKAWA Tatsuhiko 鹿児島大学, 大学院・医歯学総合研究科, 教授 (40219100)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 内因性血管新生阻害因子 / プロモーター活性化 / thrombospondin-1 / ヒト齊帯静脈血管内皮細胞 / 腫瘍血管新生

Research Abstract

In this study, we attempt to find the natural compounds which activate the promoter of "endogenous anti-angiogenesis factor" such as endostatin, angiostatin and thrombospondin. We tried to establish a bioassay method using the cells that were stably transfected by the luciferase reporter plasmid fused with the promoter domain of the thrombospondin-1 gene. However, We couldn't achieve to establish the stably transfected cells. On the other hand, on the guidance of selective growth inhibition against human umbilical vein endothelial cells (HUVECs), we searched for anti-angiogenic substances from extracts of medicinal plants. We found several active extract and tried to isolate the active components by the assay-guided separation.

Research Products

• [Journal Article] Inhibition of virulence potential of Vibrio cholerae by natural compound. (2011)
  • Author(s): Yamasaki S., Masahiro Asakura M., Neogi S.B., Hinenoya A., Iwaoka E.,Aoki S.
  • Journal Title: Indian J Med.Res. 133 Pages: 232-239
  • Peer Reviewed
• [Journal Article] Inhibition of virulence potential of Vibrio cholerae by natural compounds (2011)
  • Author(s): S.Yamasaki, S.Aoki, et al
  • Journal Title: Indian J Med Res Volume: 133 Pages: 232-239
  • Peer Reviewed
• [Journal Article] Capsaicin, a potential inhibitorof cholera toxin production in Vibrio cholerae. (2010)
  • Author(s): Chatterjee S., Asakura M., Chowdhury N., Neogi S., Sugimoto N., Haldar S., Prasad SA., Hinenoya A., Aoki S., Yamasaki S.
  • Journal Title: FEMS Microbiol Lett. 306 Pages: 54-60
  • Peer Reviewed
• [Journal Article] Capsaicin, a potential inhibitorof cholera toxin production in Vibrio cholerae (2010)
  • Author(s): Shruti C., Aoki S., Yamasaki S., et al.
  • Journal Title: FEMS Microbiology Letters 306 Pages: 54-60
  • Peer Reviewed
• [Journal Article] Structure-activity relationships study of bastadin 6, an anti-angiogenic brominated-tyrosine derivedmetabolite from marine sponge. (2008)
  • Author(s): Kotoku N.; Hiramatsu A.; Tsujita H.; Hirakawa Y.; Sanagawa M.; Aoki S.; Kobayashi M.
  • Journal Title: Arch.Pharm.(Weinheim) 341 Pages: 568-577
  • Peer Reviewed
• [Journal Article] Smenospongine, a sesquiterpene aminoquinone from a marine sponge, induces G1 arrest or apoptosis in different leukemia cells. (2008)
  • Author(s): Kong D., Aoki S., Sowa Y., Sakai T., Kobayashi M.
  • Journal Title: Mar.Drugs 6 Pages: 480-488
  • Peer Reviewed
• [Journal Article] Smenospongine, a sesquiterpene aminoquinone from a marine sponge, induces G1 arrest or apoptosis in different leukemia cells. (2008)
  • Author(s): D. Kong, S. Aoki, et al.
  • Journal Title: Mar. Drui 6 Pages: 480-488
  • Peer Reviewed
• [Journal Article] Structure-activity relationships study of bastadin 6, an anti-angiogenic brominated-tyrosine derivedmetabolite from marine sponge. (2008)
  • Author(s): N. Kotoku, S. Aoki, et al.
  • Journal Title: Arch. Pharm. 341 Pages: 568-577
  • Peer Reviewed