Development of enrichment methods of sialoglycopeptides towards glycobiomarker discovery

Research Project

Project/Area Number	20510212
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Living organism molecular science
Research Institution	National Institute of Advanced Industrial Science and Technology
Principal Investigator	KAMEYAMA Akihiko National Institute of Advanced Industrial Science and Technology, 糖鎖医工学研究センター, 研究チーム長 (80415661)
Research Collaborator	TOYODA Masaaki 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, 特別研究員
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	化学修飾 / 糖ペプチド / シアル酸 / バイオマーカー / 固相反応 / グライコミクス
Research Abstract	Glycoproteins have great potential as novel clinical biomarkers. We studied on the development of a novel enrichment method of sialoglycoproteins which contain sialic acids on their glycan moieties, as an approach to biomarker discovery focused on sialoglycoproteins. After intensive trials, we found that oxime formation is one of the most practical methods for coupling of sialoglycoproteins and resin beads. Then, we designed and synthesis of a linker which can introduce oxyamine group at sialic acid moiety.