Crystallization of Proteins based on Molecular Assembling by Photon Force
| Project/Area Number |
20550002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
TSUBOI Yasuyuki Hokkaido University, 大学院・理学研究院, 准教授 (00283698)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 光圧 / 輻射力 / 放射圧 / 結晶化 / プラズモン / 分子集合体 / アミノ酸 / リゾチーム / 金ナノ粒子 / 共焦点光学顕微鏡 / 光捕捉 / 勾配力 / SERS / 共焦点顕微鏡 / アルギニン / 光ピンセット / 近赤外レーザー / ヘム蛋白質 / シトクロム / ミオグロビン / ヘム / チトクローム / フォトンフォース / ラマン |
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| Research Abstract |
We demonstrated a novel technique to promote crystallization of hen egg-white lysozyme in heavy water. The technique was essentially based on aggregation of the protein molecules by photon pressure generated under irradiation of a cw 1064 nm laser beam to the protein solution. When photon pressure was kept being exerted for 1-2 hours on the sample solution, a small particle identified to the molecular aggregate of lysozyme was produced, as proved by in situ Raman microspectroscopy on the particle. Furthermore, we confirmed that aggregation of lysozyme by photon pressure led to crystallization of the protein.
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Report
(4 results)
Research Products
(66 results)
