The molecular conformation of amyloid fibril revealed by pressure axis

• Project/Area Number: 20550025
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Ritsumeikan University
• Principal Investigator: TANIGUCHI Yoshihiro Ritsumeikan University, 生命科学部, 教授 (70066702)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 圧力 / インシュリン / アミロイド線維 / 二次構造 / 分子構造 / FT-IR / NMR / インスリン / 変性中間体 / 温度 / 高エネルギー構造 / PLGA / FTIR分光法 / 活性化体積

Research Abstract

The pressure-induced changes in the secondary structure of insulin amyloid fibril up to 1100MPa were studied by means of the high pressure Fourier transform infrared spectroscopy. The early amyloid fibril to come from intermediate state of insulin was melted by high compression up to 550MPa. But the aged amyloid fibril does not change to be rigid structure by very high compression up to 1GPa.The detail structure of insulin under high pressure was studied by high pressure one- and two-dimensional NMR spectroscopy. High pressure NMR showed large changes in amide proton chemical shift for the residues mainly in the N- and C-terminal strands of B chain and helix1 of A-chain. The structural changes in the N- and C-terminal strands of B-chain under high pressure is a key factor for the pressure induced molecular association and aggregation of insulin

Research Products

• [Journal Article] Pressure effects on the structure, kinetic, and thermodynamic properties of heat-induced aggregation of protein studied by FTIR spectroscopy (2010)
  • Author(s): Y.Taniguchi, A.Okuno, M.Kato
  • Journal Title: J.Physics : Conference Series 215 Pages: 12156-12159
  • Peer Reviewed
• [Journal Article] Pressure effects on the structure, kinetic, and thermodynamic properties of heat-induced aggregation of protein studied by FTIR spectroscopy (2010)
  • Author(s): Y.Taniguchi, A.Okuno, M.Kato
  • Journal Title: J.Physics : Conference Series Volume: 215 Pages: 12156-12159
  • Peer Reviewed
• [Journal Article] Pressure effects on the structure, kinetics, and thermodynamic properties of heat-induced aggregation of protein studied by FT-IR spectroscopy (2010)
  • Author(s): Y.Taniguchi, A.Okuno, M.Kato
  • Journal Title: Journal of Physics : Conference Series (in press(掲載決定))
  • Peer Reviewed
• [Journal Article] High pressure FT-IR spectroscopic study on the secondary structure changes in insulin amyloid and aggregates (2009)
  • Author(s): R.Maeda, Y.Taniguchi, N.Takeda, A.Ado
  • Journal Title: High Pressure Research 29 Pages: 676-679
  • Peer Reviewed
• [Journal Article] High pressure FT-IR spectroscopic study on the secondary structure changes in insulin amyloid and aggregates (2009)
  • Author(s): Y.Taniguchi, N.Takeda, A.Ado R.Maeda
  • Journal Title: High Pressure Research 29 Pages: 676-679
  • Peer Reviewed
• [Journal Article] Uncovering the physical origin of the difference between aliphatic chain and aromatic ring in the "hydrophobic" effect on partial molar volume (2008)
  • Author(s): T.Imai, Y.Hisadomi, S.Sawamura, Y.Taniguchi
  • Journal Title: J.Chem.Phys. 128
  • Peer Reviewed
• [Journal Article] Pressure-induced helix-coil transition of poly-L-gultamic acid in water (2008)
  • Author(s): T.Takekiyo, Y.Yoshimura, A.Okuno, A.Shimizu, M.Kato, Y.Taniguchi
  • Journal Title: J.Physics Conference Series 121
  • Peer Reviewed
• [Journal Article] Effects of pressure and solvents on the infrared absorption intensities of C-I stretching modes of metliyl and ethyl iodides in solution (2008)
  • Author(s): H. Isogai, M. Kato, Y. Taniguchi
  • Journal Title: Spectrochimica Acta 69 Pages: 327-332
  • Peer Reviewed
• [Journal Article] Uncovering the physical origin of the difference between aliphatic chain and aromatic ring in the "hydrophobic"effect on partial molar volume (2008)
  • Author(s): T. Imai, Y. Hisadomi, S. Sawaniura, Y. Taniguchi
  • Journal Title: J, Chem. Phys. 128
  • Peer Reviewed
• [Journal Article] Pressure-induced helix-coil transition of poly-L-gultamic acid in water (2008)
  • Author(s): T. Takekiyo, Y. Yoshimura, A. Okuno, A. Shimizu, M. Kato, Y. Tanlguchl
  • Journal Title: J. Phys. Conf. Series 121
  • Peer Reviewed
• [Presentation] High Pressure NMR reveals a locally disordered conformation foinsulin (2010)
  • Author(s): R.Kitahara, A.Tanji, S.Kitazawa, Y.Taniguchi
  • Organizer: Pacifichem 2010
  • Place of Presentation: ハワイ(米国)
  • Year and Date: 2010-12-19
• [Presentation] High Pressure NMR reveals a locally disordered conformation of insulin (2010)
  • Author(s): R.Kitahara, A.Tanji, S.Kitazawa, Y.Taniguchi
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2010-12-19
• [Presentation] Conformational fluctuation Lys48-linked diubiquitin characterized by high pressure NMR spectroscopy (2010)
  • Author(s): R.Kitahara, T.Hirano, M.Yagi, K.Hata, Y.Taniguchi, K.Akasaka, K.Kato
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城)
  • Year and Date: 2010-09-21
• [Presentation] High-pressure NMR character izes conformational fluctuation Lys48-linked diubiquitin (2010)
  • Author(s): R.Kitahara, T.Hirano, M.Yagi, K.Hata, Y.Taniguchi, K.Akasaka, K.Kato
  • Organizer: 24^<th> International Conference on Magnetic Resonance Biological Systems
  • Place of Presentation: ケアンズ(オーストラリア)
  • Year and Date: 2010-08-23
• [Presentation] High-pressure NMR characterizes conformational fluctuation Lys48-linked diubiquitin (2010)
  • Author(s): R.Kitahara, T.Hirano, M.Yagi, K.Hata, Y Taniguchi, K.Akasaka, K.Kato
  • Organizer: 24^<th> International Conference on Magnetic Resonance Biological Systems
  • Place of Presentation: ケアンズ オーストラリア
  • Year and Date: 2010-08-23
• [Presentation] 高圧NMR法ニヨルユビキチンの高エネルギー構造に基づく構造-機能相関研究 (2010)
  • Author(s): 北原亮、北沢創一郎、坂田絵理、平野貴志、矢木内海真穂、谷口吉弘、水島恒裕、赤坂一之、加藤晃一
  • Organizer: 第10回日本蛋白質科学年会
  • Place of Presentation: 札幌コンフェレンスセンター(北海道)
  • Year and Date: 2010-06-18
• [Presentation] High pressure FT-IR spectroscopic study on the secondary structural changes in insulin amyloid fibril and aggregate (2009)
  • Author(s): Y.Taniguchi, N.Takeda, K.Ado R.Maeda
  • Organizer: XLVIIth EHPRG (European High Pressure Research Group) Conference
  • Place of Presentation: パリ(フランス)
  • Year and Date: 2009-09-08
• [Presentation] High pressure FT-IR spectroscopic study on the secondary structural changes in insulin amyloid fibril and aggregate (2009)
  • Author(s): Y.Taniguchi, N.Takeda, K.Ado R.Meada
  • Organizer: XLVIIth EHPRG(European High Pressure Research Group)Conference
  • Place of Presentation: Paris, France
• [Presentation] The secondary struct ure of pressure- and heat-induced protein aggregates and amyloid fibrils studied by high pressure FTIR spectroscopy (2008)
  • Author(s): Y.Taniguchi, A.Okuno, R.Yokoyama, M.Kato
  • Organizer: 5^<th> International Conference on High Pressure Bioscience and Biotechnology
  • Place of Presentation: Scripps Institution of Oceanography,サンデイゴ (米国)
  • Year and Date: 2008-09-18
• [Presentation] The secondary structure of pressure-and heat-induced protein aggreg es and amyloid fibrils studied by high pressure FTIR spectroscopy. (2008)
  • Author(s): Y. Taniguchi, A. Okuno, R. Yokoyama, M. Kato
  • Organizer: 5^<th> International Conference on High Pressure Bioscience an Biotechnology
  • Place of Presentation: Scripps Institution of Oceanography, San Diego USA
• [Book] 物理化学[第2版] (2011)
  • Author(s): 近藤和生・上野正勝・芝田隼次・木村隆良・谷口吉弘
  • Total Pages: 165
  • Publisher: 朝倉書店