Development of High-Performance Two-Dimensional Electrophoresis Using Low-Concentration Gel and Its Application
| Project/Area Number |
20550072
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ODAKE Tamao 東京大学, 大学院・工学系研究科, 研究員 (10301128)
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| Co-Investigator(Kenkyū-buntansha) |
TSUNODA Kin-ichi 群馬大学, 大学院・工学研究科, 教授 (30175468)
SAHEKI Toshihiko 群馬大学, 大学院・工学研究科, 助教 (00241860)
UEKI Yuji 日本原子力研究開発機構, 量子ビーム応用研究部門, 研究員 (50446415)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 分離分析 / 電気泳動 / 二次元電気泳動 / 等電点電気泳動 / タンパク質 / アクリルアミド / 低濃度ゲル / 固定化pH勾配 / メッシュチューブ / プロテオーム解析 / マルチフィラメント糸 / 放射線重合 |
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| Research Abstract |
The researchers have developed a new isoelectric focusing(IEF) gel supported by a multifilament as a first-dimensional IEF gel of two-dimensional electrophoresis(2-DE), which can separate proteins with high-resolution. The multifilament-supporting(MFS) gel could overcome difficulty in handling the IEF gel, prepare low-concentration gel to separate high-molecular-weight proteins. In this study, to improve operability and reproducibility, support of the low-concentration gel, method of polymerization, and introduction of immobilized pH gradient were investigated. The 2-DE system using the low-concentration MFS gel was proved to be useful for proteome analysis.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Miniaturized two-dimensional gel electrophoresis of high-molecular-weight proteins using low-concentration multifilament-supporting gel for isoelectric focusing2009
Author(s)
Tamao Odake, Toshihiko Saheki, Kazutoshi Kamezawa, Kozue Miyata, Hiromi Takiguchi, Hiroki Hotta, Koki Onda, Yasuaki Kojima, Jinxiang Li, Kin-ichi Tsunoda
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Journal Title
Journal of Electrophoresis
Volume: 53
Issue: 4
Pages: 57-61
DOI
NAID
ISSN
1349-9394, 1349-9408
Related Report
Peer Reviewed
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