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Development of high-resolution determination methods for nanoparticles

Research Project

Project/Area Number 20550073
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionGifu University

Principal Investigator

TAKEUCHI Toyohide  Gifu University, 工学部, 教授 (40135322)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords液体クロマトグラフィー / ハイドロダイナミッククロマトグラフィー / サイズ排除クロマトグラフィー / シリカコロイド / 金コロイド / ハイドロダイナミックモード / サイズ排除モード / ハイドダイナミックモード
Research Abstract

Hydrodynamic chromatography (HDC) conditions have been examined for the separation of silica colloids with 5-78 nm diameters. Conventional HPLC columns with 25 cm×4.6 mm I.D. were prepared and employed for HDC.
Interstitial spaces among packing materials are usually employed in HDC. Considering the size of silica colloids, non-porous silica gel with 2-5 μm was packed for the HDC separation of silica colloids. Eluents for HDC of silica colloids were examined in detail, and phosphate buffer solutions with pH larger than 7 gave reasonable elution volumes for silica colloids. A 10 mM phosphate buffer with pH 7.1 was the optimum eluent for silica colloids. The separation column with smaller particle diameter provided better resolution for silica colloids. The silica colloids were detected with a UV detector based on turbidimetry, and the larger colloid gave larger signal intensity.
The elution volume of silica colloids slightly increased with decreasing flow rate. It was speculated that lower … More flow rate enhanced the diffusion of analytes into narrower channels.
It is required to use smaller particles or to use a longer column in order to improve the resolution. However, higher pressure drop over such separation columns are indispensable, and preparation of such columns is not easy. Since monolithic silica columns having higher permeability and better resolution will overcome these drawbacks, hydrodynamic capillary chromatography (HDCC) was examined for the separation of colloidal particles.
Monolithic silica capillary columns with 50-200 μm were prepared and examined for the HDCC separation of colloidal silica and gold nanoparticles with 13-61 nm particle diameters. It was found that smaller elution volumes were observed for larger nanoparticles. In order to minimize the extra-column dispersion, the injection volume was reduced to 20 nL or less, and the on-column detection was carried out. It was expected that the monolithic silica capillary columns possessed mesopore with 10-15 nm and size-exclusion effect could be involved for the elution of smaller nanoparticles. Furthermore, eluents were examined in detail so as not to cause adsorption and coagulation of nanoparticles while passing through the separation column and connecting tubes. It was found that phosphate buffers with pH 7 were good for silica colloids, while aqueous sodium dodecylsulfate solutions were good for gold colloids. Monolithic silica capillary columns with 50 cm in length provided resolution comparable to 25-cm packed columns. It was expected that longer monolithic silica capillary columns would achieve better resolution. Less

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (14 results)

All 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (12 results)

  • [Journal Article] Hydrodynamic chromatography of silica colloids on small spherical nonporous silica particles2009

    • Author(s)
      T.Takeuchi, Siswoyo, Z.Aspanut, L.W.Lim
    • Journal Title

      Anal.Sci. Vol. 25

      Pages: 301-306

    • NAID

      10025210582

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hydrodynamic chromatography of silica colloids on small spherical nonporous silica particles2009

    • Author(s)
      T. Takeuchi, Siswoyo, Z. Aspanut, L. W. Lim
    • Journal Title

      Analytical Sciences 25

      Pages: 301-306

    • NAID

      10025210582

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] モノリス型キャピラリーカラムを用いたナノ粒子のサイズ分離手法の開発2010

    • Author(s)
      加藤くみ子・斎藤栄子・竹内豊英・川西徹
    • Organizer
      第21回クロマトグラフィー科学会議
    • Place of Presentation
      武庫川女子大学
    • Year and Date
      2010-10-23
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] separation by hydrodynamic chromatography2010

    • Author(s)
      Siswoyo, Lim Lee Wah, 竹内豊英
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Synthesis of gold nano colloids and their separation by hydrodynamic chromatography2010

    • Author(s)
      Siswoyo, Lim Lee Wah, 竹内豊英
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Separation of colloidal species by hydrodynamic chromatography2009

    • Author(s)
      シスウォヨ・リムリーワ・竹内豊英
    • Organizer
      第40回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      岐阜大学
    • Year and Date
      2009-11-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Separation of colloidal species by hydrodynamic chromatogrphy2009

    • Author(s)
      シスウォヨ・リムリーワ・竹内豊英
    • Organizer
      第40回中部化学関係協会支部連合秋季大会
    • Place of Presentation
      岐阜大学
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 高分解能キャピラリーサイズ排除クロマトグラフィーによるシリカコロイドの分離検出2009

    • Author(s)
      西川周二・リムリーワ・竹内豊英
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 高分解能キャピラリーサイズ排除クロマトグラフィーによるシリカコロイドの分離検出2009

    • Author(s)
      西川周二・リムリーワ・竹内豊英
    • Organizer
      日本分析学会第58年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] シリカコロイドのハイドロダイナミッククロマトグラフィー2008

    • Author(s)
      竹内豊英・Siswoyo・リムリーワ
    • Organizer
      第19 回クロマトグラフィー科学会議
    • Place of Presentation
      京都大学桂キャンパス
    • Year and Date
      2008-12-01
    • Related Report
      2010 Final Research Report
  • [Presentation] シリカコロイドのハイドロダイナミッククロマトグラフィー2008

    • Author(s)
      竹内豊英, Siswoyo, リムリーワ
    • Organizer
      第19回クロマトグラフィー科学会議
    • Place of Presentation
      東京大学桂キャンバス
    • Year and Date
      2008-12-01
    • Related Report
      2008 Annual Research Report
  • [Presentation] ハイドロダイナミッククロマトグラフィーによるシリカコロイドの分離定量2008

    • Author(s)
      竹内豊英・Siswoyo・リムリーワ
    • Organizer
      Separation Sciences 2008
    • Place of Presentation
      東京理科大学薬学部
    • Year and Date
      2008-11-13
    • Related Report
      2010 Final Research Report
  • [Presentation] ハイドロダイナミッククロマトグラフィーによるシリカニロイドの分離定量2008

    • Author(s)
      竹内豊英, Siswoyo, リムリーワ
    • Organizer
      Separation Seiences 2008
    • Place of Presentation
      東京理工大学薬学部
    • Year and Date
      2008-11-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Separation of nano colloidal silica by hydrodynamic chromatography2008

    • Author(s)
      Siswoyo・Lim Lee Wah・竹内豊英
    • Organizer
      日本分析化学会第57年会
    • Place of Presentation
      福岡大学
    • Year and Date
      2008-09-10
    • Related Report
      2010 Final Research Report 2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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