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Development of the Electrochemical Separation/Detection System for Proteins Using the Oil/Water Interface

Research Project

Project/Area Number 20550076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionKobe University

Principal Investigator

OSAKAI Toshiyuki (2009-2010)  Kobe University, 大学院・理学研究科, 准教授 (30183023)

大堺 利行 (2008)  Kobe University, 理学研究科, 准教授 (00194118)

Research Collaborator KATANO Hajime  福井県立大学, 生物資源学部, 教授
SUGIHARA Takayasu  住友電気工業(株)・解析技術研究センター
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsタンパク質 / 液液界面 / フローセル / 電気化学測定 / 尿中タンパク質 / バイオセンサー / クロマトグラフィー
Research Abstract

At the interface between an oil phase containing certain anionic surfactants and an aqueous phase (pH〓3.4) containing proteins, the adsorption of the proteins is facilitated by complexation with the surfactants. This reaction is dependent on the potential difference of the oil/water interface, and the electric current flowing through the interface is proportional to the protein concentration in the presence of excess surfactant, suggesting the possibility of qualitative and quantitative analyses of proteins. Based on such measurement principles, we could develop a flow separation/detection system for proteins.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (40 results)

All 2010 2009 2008 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (26 results) Remarks (4 results)

  • [Journal Article] Flow-Injection On-line Electrochemical Separation/ Determination of Ions Using a Two-Step Oil/Water-Type Flow Cell System2010

    • Author(s)
      E.Gohara, T.Osakai
    • Journal Title

      Anal.Sci. Vol.26, No.3

      Pages: 375-378

    • NAID

      10026782578

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct Label-free Electro-chemical Detection of Proteins Using the Polarized Oil/Water Interface2010

    • Author(s)
      T.Osakai, Y.Yuguchi, E.Gohara, H.Katano
    • Journal Title

      Langmuir Vol.26, No.13

      Pages: 11530-11537

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct Label-free Electrochemical Detection of Proteins Using the Polarized Oil/Water Interface2010

    • Author(s)
      T.Osakai
    • Journal Title

      Langmuir

      Volume: 26巻 Pages: 11530-11537

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flow-Injection On-line Electrochemical Separation/Determination of Ions Using a Two-Step Oil/Water-Type Flow Cell System2010

    • Author(s)
      E.Gohara
    • Journal Title

      Anal.Sci. 26

      Pages: 375-378

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bimolecular-Reaction Effect on the Rate Constant of Electron Transfer at the Oil/Water Interface as Studied by Scanning Electrochemical Microscopy2009

    • Author(s)
      T.Osakai
    • Journal Title

      J.Electroanal.Chem. 628

      Pages: 27-34

    • NAID

      120001460042

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential-Modulated Fluorescence Spectroscopy of the Membrane Potential-Sensitive Dye Di-4-ANEPPS at the 1,2-Dichloroethane/Water Interface2009

    • Author(s)
      T.Osakai
    • Journal Title

      Anal.Bioanal.Chem. 395

      Pages: 1055-1061

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kinetic Analysis of Electron Transfer across Single Water-Microdroplet/Oil and Oil-Microdroplet/Water Interfaces2009

    • Author(s)
      K. Nakatani
    • Journal Title

      Anal. Sci. 25

      Pages: 183-187

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bimolecular-Reaction Effect on the Rate Constant of Electron Transfer at the Oil/Water Interface as Studied by Scanning Electrochemical Microscopy2009

    • Author(s)
      T. Osakai
    • Journal Title

      J. Electroanal. Chem. 628

      Pages: 27-34

    • NAID

      120001460042

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electro-chemical Aspects of the Reverse Micelle Extraction of Proteins2008

    • Author(s)
      T.Osakai, A.Shinohara
    • Journal Title

      Anal.Sci. Vol.24, No.7

      Pages: 901-906

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Aspects of the Reverse Micelle Extraction of Proteins2008

    • Author(s)
      T. Osakai
    • Journal Title

      Anal. Sci. 24

      Pages: 901-906

    • NAID

      10025205128

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Electron Transfer Mediated by Membrane-bound D-Fructose Dehydro-genase Adsorbed at an Oil/Water interface2010

    • Author(s)
      佐々木優子
    • Organizer
      2010環太平洋国際化学会議
    • Place of Presentation
      ホノルル(USA).
    • Year and Date
      2010-12-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Electron Transfer Mediated by Membrane-bound D-Fructose Dehydrogenase Adsorbed at an Oil/Water2010

    • Author(s)
      Y.Sasaki
    • Organizer
      2010環太平洋国際化学会議
    • Place of Presentation
      ホノルル(USA)
    • Year and Date
      2010-12-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 油水界面に吸着したフルクトースデヒドロゲナーゼによる電子移動反応2010

    • Author(s)
      佐々木優子
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      秋田大学.
    • Year and Date
      2010-11-06
    • Related Report
      2010 Final Research Report
  • [Presentation] 油水界面に吸着したフルクトースデヒドロゲナーゼによる電子移動反応2010

    • Author(s)
      佐々木優子
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      秋田大学
    • Year and Date
      2010-11-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 油水界面でのシトクロームcの電子移動反応のメカニズム2010

    • Author(s)
      今井蓉子
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学.
    • Year and Date
      2010-09-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 油水界面でのシトクロームcの電子移動反応のメカニズム2010

    • Author(s)
      今井蓉子
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 油水界面に吸着したフルクトースデヒドロゲナーゼによる電子移動反応2010

    • Author(s)
      佐々木優子
    • Organizer
      第71回分析化学討論会
    • Place of Presentation
      島根大学.
    • Year and Date
      2010-05-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 油水界面に吸着したフルクトースデヒドロゲナーゼによる電子移動反応2010

    • Author(s)
      佐々木優子
    • Organizer
      第71回分析化学討論会
    • Place of Presentation
      島根大学
    • Year and Date
      2010-05-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 油水界面型フローセルを用いるイオンの分離定量法の開発2009

    • Author(s)
      郷原絵美
    • Organizer
      第55回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      徳島大学.
    • Year and Date
      2009-11-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 油水界面型フローセルを用いるイオンの分離定量法の開発2009

    • Author(s)
      郷原絵美
    • Organizer
      第55回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      徳島大学(徳島県)
    • Year and Date
      2009-11-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 油水界面型フローセルを用いる血清中リチウムイオンの定量法の開発2009

    • Author(s)
      郷原絵美
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学.
    • Year and Date
      2009-09-25
    • Related Report
      2010 Final Research Report
  • [Presentation] 油水界面型フローセルを用いる血清中リチウムイオンの定量法の開発2009

    • Author(s)
      郷原絵美
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Label-free Electrochemical Detection of Proteins Using the Oil/ Water Interface2009

    • Author(s)
      T.Osakai
    • Organizer
      the 42nd Heyrovsky Discussion'Liquid-liquid Electro-chemistry-from Fundamentals toApplications
    • Place of Presentation
      Trest, Czech.
    • Year and Date
      2009-06-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Label-free Electrochemical Detection of Proteins Using the Oil/Water Interface2009

    • Author(s)
      T.Osakai
    • Organizer
      The 42nd Heyrovsky Discussion
    • Place of Presentation
      Trest, Czech
    • Year and Date
      2009-06-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 油水界面型フローセルを用いたタンパク質の分離定量法の開発2009

    • Author(s)
      郷原絵美
    • Organizer
      第70回分析化学討論会
    • Place of Presentation
      和歌山大学.
    • Year and Date
      2009-05-16
    • Related Report
      2010 Final Research Report
  • [Presentation] 油水界面型フローセルを用いたタンパク質の分離定量法の開発2009

    • Author(s)
      郷原絵美
    • Organizer
      第70回分析化学討論会
    • Place of Presentation
      和歌山大学(和歌山県)
    • Year and Date
      2009-05-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] 油水界面を用いるタンパク質のラベルフリー電気化学検出の試み2009

    • Author(s)
      大堺利行
    • Organizer
      電気化学会第76回大会
    • Place of Presentation
      京都大学.
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 油水界面を用いるタンパク質のラベルフリー電気化学検出の試み2009

    • Author(s)
      大堺利行
    • Organizer
      電気化学会第76回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 油水界面型全電解フローセルを用いるイオンの分離定量法の開発2008

    • Author(s)
      郷原絵美
    • Organizer
      第54回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      熊本大学.
    • Year and Date
      2008-11-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 油水界而型全電解フローセルを用いるイオンの分離定量法の開発2008

    • Author(s)
      郷原絵美
    • Organizer
      第54回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      熊本大学
    • Year and Date
      2008-11-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] アニオン性界面活性剤存在下の油水界面におけるタンパク質のボルタンメトリーとラベルフリー検出への応用2008

    • Author(s)
      湯口友紀子
    • Organizer
      第54回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      熊本大学.
    • Year and Date
      2008-11-22
    • Related Report
      2010 Final Research Report
  • [Presentation] アニオン性界面活性剤存在下の油水界面におけるタンパク質のボルタンメトリーとラベルフリー検出への応用2008

    • Author(s)
      湯口友紀子
    • Organizer
      第54回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      熊本大学
    • Year and Date
      2008-11-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] 油水界面型全電解フローセルを用いるアセチルコリンおよびコリンの同時定量2008

    • Author(s)
      郷原絵美
    • Organizer
      日本分析化学会第57年会
    • Place of Presentation
      福岡大学.
    • Year and Date
      2008-09-10
    • Related Report
      2010 Final Research Report
  • [Presentation] 油水界面型全電解フローセルを用いるアセチルコリンおよびコリンの同時定量2008

    • Author(s)
      郷原絵美
    • Organizer
      日本分析化学会第57年会
    • Place of Presentation
      福岡大学
    • Year and Date
      2008-09-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] アニオン性界面活性剤を用いるタンパク質の電気化学的検出法の検討2008

    • Author(s)
      湯口友紀子
    • Organizer
      第69回分析化学討論会
    • Place of Presentation
      名古屋国際会議場.
    • Year and Date
      2008-05-15
    • Related Report
      2010 Final Research Report
  • [Presentation] アニオン性界面活性剤を用いるタンパク質の電気化学的検出法の検討2008

    • Author(s)
      湯口友紀子
    • Organizer
      第69回分析化学討論会
    • Place of Presentation
      名古屋團際会議場
    • Year and Date
      2008-05-15
    • Related Report
      2008 Annual Research Report
  • [Remarks] 研究室のホームページ

    • URL

      http://www2.kobe-u.ac.jp/~osakai/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www2.kobe-u.ac.jp/~osakai/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www2.kobe-u.ac.jp/~osakai/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www2.kobe-u.ac.jp/~osakai/

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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