Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Research Abstract |
The ultrasound sonolytic hydrolysis of peptides was performed with added benzenethiol as a hydrogen-releasing reagent in aqueous solution. The ultrasound hydrolysis of peptides differed from that due to conventional acid hydrolysis in characteristics, i.e., cleavage at proline residue and dehydration as a side reaction. The sonolysis combined with the thiol addition made it possible to determine the amino-acid sequence of peptides. Furthermore, new reagent for releasing hydrogen atoms was found out in the experiment of the irradiation of ultraviolet laser photon in vacuum condition.
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