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Specific degradation of peptides with hydrogen radical and its application to protein chemistry

Research Project

Project/Area Number 20550081
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionYokohama City University

Principal Investigator

TAKAYAMA Mitsuo  Yokohama City University, 生命ナノシステム科学研究科, 教授 (10328635)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords水素ラジカル / 質量分析 / タンパク質 / 分解 / 還元反応 / 抗酸化 / 超音波 / 加水分解
Research Abstract

The ultrasound sonolytic hydrolysis of peptides was performed with added benzenethiol as a hydrogen-releasing reagent in aqueous solution. The ultrasound hydrolysis of peptides differed from that due to conventional acid hydrolysis in characteristics, i.e., cleavage at proline residue and dehydration as a side reaction. The sonolysis combined with the thiol addition made it possible to determine the amino-acid sequence of peptides. Furthermore, new reagent for releasing hydrogen atoms was found out in the experiment of the irradiation of ultraviolet laser photon in vacuum condition.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (19 results)

All 2010 2009 2008 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (9 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] In-source decay and fragmentation characteristics of peptides using 5-aminosalicylic acid as a matrix in matrix-assisted laser desorption/ionization mass spectrometry Journal of the American Society for Mass Spectrometry2010

    • Author(s)
      M.Sakakura, M.Takayama
    • Journal Title

      J.Am.Soc.Mass Spectrom. Vol.21

      Pages: 979-988

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of charge state and amino acid composition on hydrogen transfer in electron capture dissociation of peptides2010

    • Author(s)
      T.Nishikaze, M.Takayama
    • Journal Title

      J.Am.Soc.Mass Spectrom Vol.21

      Pages: 1979-1988

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In-source decay and fragmentation characteristics of peptides using 5-aminosahcylic acid as a matrix in matrix-assisted laser desorption/ionization mass spectrometry2010

    • Author(s)
      M.Sakakura, M.Takayama
    • Journal Title

      Journal of the American Society for Mass Spectrometry

      Volume: 21 Pages: 979-988

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of charge state and amino acid composition on hydrogen transfer in electron capture dissociation of peptides2010

    • Author(s)
      T.Nishikaze, M.Takayama
    • Journal Title

      Journal of the American Society for Mass Spectrometry

      Volume: 21 Pages: 1979-1988

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In-source decay and fragmentation characteristics of peptides using 5-a minosalicylic acid as a matrix in matrix-assisted laser desorption/ionization mass spectrometry2010

    • Author(s)
      M.Sakakura, M.Takayama
    • Journal Title

      Journal of the American Society for Mass Spectrometry (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sonolytic hydrolysis of peptides in aqueous solution upon addition of catechol2009

    • Author(s)
      M.Sakakura, M.Takayama
    • Journal Title

      Ultrasonics Sonochemistry Vol.16

      Pages: 367-371

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sonolytic hydrolysis of peptides in aqueous solution upon addition of catechol2009

    • Author(s)
      M. Sakakura, M. Takayama
    • Journal Title

      Ultrasonics Sonochemistry 16

      Pages: 367-371

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] A new matrix suitable for MALDI-ISD of phosphorylated peptides2010

    • Author(s)
      M.Sakakura, M.Takayama
    • Organizer
      58th ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Salt lake city, Utah, USA
    • Year and Date
      2010-05-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] A new matrix suitable for MALDI-ISD of phosphorylated peptides2010

    • Author(s)
      坂倉幹始, 高山光男
    • Organizer
      the 58th ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Salt lake city, Utah, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Comparative study of hydrogen-induced cleavage of peptides with MALDI-ISD and sonolysis2009

    • Author(s)
      坂倉幹始・高山光男
    • Organizer
      18^<th> International Mass Spectrometry Conference
    • Place of Presentation
      Bremen, Germany
    • Year and Date
      2009-09-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] ペプチド用新規MALDIマトリックスとインソース分解の評価2009

    • Author(s)
      坂倉幹始・高山光男
    • Organizer
      日本ヒトプロテオーム機構
    • Place of Presentation
      北里大学
    • Year and Date
      2009-07-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 超音波を用いたペプチドの分解反応とその特性2009

    • Author(s)
      坂倉幹始・高山光男
    • Organizer
      第70回分析化学討論会
    • Place of Presentation
      和歌山大学
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Comparative study of hydrogen-induced cleavage of peptides with MALDI-ISD and sonolysis2009

    • Author(s)
      坂倉幹始, 高山光男
    • Organizer
      18th International Mass Spectrometry Conference
    • Place of Presentation
      Bremen, Germany
    • Related Report
      2010 Final Research Report
  • [Presentation] 超音波によるペプチドの加水分解反応の特徴2008

    • Author(s)
      坂倉幹始, 高山光男
    • Organizer
      第17回ソノケミストリー討論会
    • Place of Presentation
      電気通信大学
    • Year and Date
      2008-12-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] 水素ラジカルと超音波を利用したペプチドの分解反応のMALDI-TOFMS解析2008

    • Author(s)
      坂倉幹始, 高山光男
    • Organizer
      第56回質量分析総合討論会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2008-05-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] 超音波によるペプチドの加水分解反応の特徴2008

    • Author(s)
      坂倉幹始, 高山光男
    • Organizer
      第17回ソノケミストリー討論会
    • Place of Presentation
      電気通信大学,東京
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://masspecs.sci.yokohama-cu.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] ペプチド主鎖N-Cα結合の特異的切断方法2009

    • Inventor(s)
      高山光男・坂倉幹始
    • Industrial Property Rights Holder
      横浜市立大学
    • Industrial Property Number
      2009-167550
    • Filing Date
      2009-07-16
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] チオール化合物と超音波を利用したペプチドの加水分解方法2008

    • Inventor(s)
      高山光男
    • Industrial Property Rights Holder
      横浜市立大学
    • Industrial Property Number
      2008-316326
    • Filing Date
      2008-12-12
    • Related Report
      2010 Final Research Report 2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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